Restriction map for
pF1KB5951
(Linear) (Six-base) MAPSORT of: KIBB5951.seq Check: 9106 from: 1 to: 1530
KIBB5951.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 0 1331 1530
Size: 1331 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 197 1087 1420 1530
Size: 149 48 890 333 110
Fragments arranged by size:
890 333 149 110 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 506 587 1530
Size: 506 81 943
Fragments arranged by size:
943 506 81
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 737 769 806 838 1530
Size: 191 32 514 32 37 32 692
Fragments arranged by size:
692 514 191 37 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 550 600 1119 1530
Size: 550 50 519 411
Fragments arranged by size:
550 519 411 50
ApaI G_GGCC'C
Cuts at: 0 520 842 1079 1530
Size: 520 322 237 451
Fragments arranged by size:
520 451 322 237
ApoI r'AATT_y
Cuts at: 0 1336 1530
Size: 1336 194
AvaI C'yCGr_G
Cuts at: 0 825 887 1530
Size: 825 62 643
Fragments arranged by size:
825 643 62
BanII G_rGCy'C
Cuts at: 0 520 842 1079 1530
Size: 520 322 237 451
Fragments arranged by size:
520 451 322 237
BbsI GAAGACnn'nnnn_
Cuts at: 0 1466 1530
Size: 1466 64
BbvCI CC'TCA_GC
Cuts at: 0 692 1423 1530
Size: 692 731 107
Fragments arranged by size:
731 692 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 868 902 1504 1530
Size: 868 34 602 26
Fragments arranged by size:
868 602 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 644 1530
Size: 644 886
BclI T'GATC_A
Cuts at: 0 15 1530
Size: 15 1515
BglII A'GATC_T
Cuts at: 0 111 1530
Size: 111 1419
BlpI GC'TnA_GC
Cuts at: 0 1264 1530
Size: 1264 266
Bme1580I G_kGCm'C
Cuts at: 0 520 842 1079 1530
Size: 520 322 237 451
Fragments arranged by size:
520 451 322 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 496 716 1530
Size: 496 220 814
Fragments arranged by size:
814 496 220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1289 1530
Size: 1289 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 939 1337 1530
Size: 728 211 398 193
Fragments arranged by size:
728 398 211 193
Bpu10I CC'TnA_GC
Cuts at: 0 692 1423 1530
Size: 692 731 107
Fragments arranged by size:
731 692 107
BsaWI w'CCGG_w
Cuts at: 0 1200 1530
Size: 1200 330
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 1530
Size: 268 30 1232
Fragments arranged by size:
1232 268 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 276 680 1444 1530
Size: 276 404 764 86
Fragments arranged by size:
764 404 276 86
BseYI C'CCAG_C
Cuts at: 0 843 1140 1220 1530
Size: 843 297 80 310
Fragments arranged by size:
843 310 297 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1530
Size: 259 1271
BsmI GAATG_Cn'
Cuts at: 0 67 1530
Size: 67 1463
Bsp1286I G_dGCh'C
Cuts at: 0 520 842 1079 1530
Size: 520 322 237 451
Fragments arranged by size:
520 451 322 237
BsrBI CCG'CTC
Cuts at: 0 1271 1530
Size: 1271 259
BsrDI GCAATG_nn'
Cuts at: 0 1157 1530
Size: 1157 373
BsrFI r'CCGG_y
Cuts at: 0 1238 1530
Size: 1238 292
BstEII G'GTnAC_C
Cuts at: 0 1122 1530
Size: 1122 408
BstYI r'GATC_y
Cuts at: 0 111 144 987 1530
Size: 111 33 843 543
Fragments arranged by size:
843 543 111 33
Bsu36I CC'TnA_GG
Cuts at: 0 458 477 776 1530
Size: 458 19 299 754
Fragments arranged by size:
754 458 299 19
BtgI C'CryG_G
Cuts at: 0 1367 1530
Size: 1367 163
EaeI y'GGCC_r
Cuts at: 0 1240 1530
Size: 1240 290
EarI CTCTTCn'nnn_
Cuts at: 0 125 1199 1366 1530
Size: 125 1074 167 164
Fragments arranged by size:
1074 167 164 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 197 728 939 1087 1337 1420 1530
Size: 149 48 531 211 148 250 83 110
Fragments arranged by size:
531 250 211 149 148 110 83 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 369 695 1426 1530
Size: 369 326 731 104
Fragments arranged by size:
731 369 326 104
EcoO109I rG'GnC_Cy
Cuts at: 0 517 822 838 839 1075 1076 1530
Size: 517 305 16 1 236 1 454
Fragments arranged by size:
517 454 305 236 16 1 1
HaeII r_GCGC'y
Cuts at: 0 1221 1530
Size: 1221 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 283 315 1530
Size: 205 32 46 32 1215
Fragments arranged by size:
1215 205 46 32 32
HincII GTy'rAC
Cuts at: 0 154 1530
Size: 154 1376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1530
Size: 277 1253
MspA1I CmG'CkG
Cuts at: 0 496 1107 1446 1493 1530
Size: 496 611 339 47 37
Fragments arranged by size:
611 496 339 47 37
NcoI C'CATG_G
Cuts at: 0 1367 1530
Size: 1367 163
NdeI CA'TA_TG
Cuts at: 0 978 1530
Size: 978 552
NspI r_CATG'y
Cuts at: 0 487 834 1530
Size: 487 347 696
Fragments arranged by size:
696 487 347
PasI CC'CwG_GG
Cuts at: 0 40 1530
Size: 40 1490
PflMI CCAn_nnn'nTGG
Cuts at: 0 550 1530
Size: 550 980
PfoI T'CCnGG_A
Cuts at: 0 705 1376 1478 1530
Size: 705 671 102 52
Fragments arranged by size:
705 671 102 52
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1080 1112 1530
Size: 1080 32 418
Fragments arranged by size:
1080 418 32
PpuMI rG'GwC_Cy
Cuts at: 0 822 1530
Size: 822 708
PshAI GACnn'nnGTC
Cuts at: 0 663 1530
Size: 663 867
PspOMI G'GGCC_C
Cuts at: 0 516 838 1075 1530
Size: 516 322 237 455
Fragments arranged by size:
516 455 322 237
PstI C_TGCA'G
Cuts at: 0 495 605 762 1498 1530
Size: 495 110 157 736 32
Fragments arranged by size:
736 495 157 110 32
PvuII CAG'CTG
Cuts at: 0 496 1107 1530
Size: 496 611 423
Fragments arranged by size:
611 496 423
SanDI GG'GwC_CC
Cuts at: 0 822 1530
Size: 822 708
SapI GCTCTTCn'nnn_
Cuts at: 0 125 1530
Size: 125 1405
SexAI A'CCwGG_T
Cuts at: 0 230 1530
Size: 230 1300
SfcI C'TryA_G
Cuts at: 0 491 601 758 1494 1530
Size: 491 110 157 736 36
Fragments arranged by size:
736 491 157 110 36
SmaI CCC'GGG
Cuts at: 0 827 1530
Size: 827 703
SphI G_CATG'C
Cuts at: 0 487 834 1530
Size: 487 347 696
Fragments arranged by size:
696 487 347
StyI C'CwwG_G
Cuts at: 0 1367 1530
Size: 1367 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 441 445 1530
Size: 441 4 1085
Fragments arranged by size:
1085 441 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 839 871 1530
Size: 268 32 539 32 659
Fragments arranged by size:
659 539 268 32 32
XmaI C'CCGG_G
Cuts at: 0 825 1530
Size: 825 705
Enzymes that do cut:
AccI AcuI AlfI AloI AlwNI ApaI ApoI AvaI
BanII BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BsrBI BsrDI BsrFI BstEII
BstYI Bsu36I BtgI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII MmeI MspA1I NcoI NdeI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuII SanDI SapI SexAI SfcI SmaI SphI StyI
TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BfrBI BglI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI
ZraI