Restriction map for
pF1KB5948
(Linear) (Six-base) MAPSORT of: KIBB5948.seq Check: 5666 from: 1 to: 1632
KIBB5948.orf
With 182 enzymes: SgfI *
November 17, 2014 16:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 137 203 533 1632
Size: 59 78 66 330 1099
Fragments arranged by size:
1099 330 78 66 59
AleI CACnn'nnGTG
Cuts at: 0 1264 1632
Size: 1264 368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1116 1632
Size: 1116 516
AlwNI CAG_nnn'CTG
Cuts at: 0 484 841 1632
Size: 484 357 791
Fragments arranged by size:
791 484 357
ApaLI G'TGCA_C
Cuts at: 0 437 1632
Size: 437 1195
ApoI r'AATT_y
Cuts at: 0 268 278 370 938 1632
Size: 268 10 92 568 694
Fragments arranged by size:
694 568 268 92 10
AseI AT'TA_AT
Cuts at: 0 925 932 1632
Size: 925 7 700
Fragments arranged by size:
925 700 7
AvaI C'yCGr_G
Cuts at: 0 1183 1632
Size: 1183 449
BanI G'GyrC_C
Cuts at: 0 1287 1632
Size: 1287 345
BanII G_rGCy'C
Cuts at: 0 1445 1632
Size: 1445 187
BciVI GTATCCnnnnn_n'
Cuts at: 0 439 1632
Size: 439 1193
BglII A'GATC_T
Cuts at: 0 206 749 1632
Size: 206 543 883
Fragments arranged by size:
883 543 206
Bme1580I G_kGCm'C
Cuts at: 0 297 441 1230 1632
Size: 297 144 789 402
Fragments arranged by size:
789 402 297 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1429 1560 1632
Size: 1421 8 131 72
Fragments arranged by size:
1421 131 72 8
Bpu10I CC'TnA_GC
Cuts at: 0 1241 1632
Size: 1241 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 1632
Size: 1519 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 1060 1343 1632
Size: 1060 283 289
Fragments arranged by size:
1060 289 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 287 317 1632
Size: 76 30 181 30 1315
Fragments arranged by size:
1315 181 76 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 29 176 760 1577 1632
Size: 29 147 584 817 55
Fragments arranged by size:
817 584 147 55 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1632
Size: 1117 515
BsiHKAI G_wGCw'C
Cuts at: 0 441 1445 1632
Size: 441 1004 187
Fragments arranged by size:
1004 441 187
Bsp1286I G_dGCh'C
Cuts at: 0 297 441 1230 1445 1632
Size: 297 144 789 215 187
Fragments arranged by size:
789 297 215 187 144
BspHI T'CATG_A
Cuts at: 0 947 1632
Size: 947 685
BsrDI GCAATG_nn'
Cuts at: 0 295 626 860 1632
Size: 295 331 234 772
Fragments arranged by size:
772 331 295 234
BsrGI T'GTAC_A
Cuts at: 0 698 1632
Size: 698 934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1109 1632
Size: 1109 523
BstXI CCAn_nnnn'nTGG
Cuts at: 0 290 1632
Size: 290 1342
BstYI r'GATC_y
Cuts at: 0 206 749 1522 1632
Size: 206 543 773 110
Fragments arranged by size:
773 543 206 110
Bsu36I CC'TnA_GG
Cuts at: 0 1292 1632
Size: 1292 340
BtgI C'CryG_G
Cuts at: 0 107 1632
Size: 107 1525
DraI TTT'AAA
Cuts at: 0 1487 1556 1632
Size: 1487 69 76
Fragments arranged by size:
1487 76 69
EaeI y'GGCC_r
Cuts at: 0 881 1632
Size: 881 751
EarI CTCTTCn'nnn_
Cuts at: 0 17 113 218 521 1632
Size: 17 96 105 303 1111
Fragments arranged by size:
1111 303 105 96 17
EcoICRI GAG'CTC
Cuts at: 0 1443 1632
Size: 1443 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 137 203 533 1421 1429 1560 1632
Size: 59 78 66 330 888 8 131 72
Fragments arranged by size:
888 330 131 78 72 66 59 8
EcoO109I rG'GnC_Cy
Cuts at: 0 786 1632
Size: 786 846
EcoRI G'AATT_C
Cuts at: 0 268 278 1632
Size: 268 10 1354
Fragments arranged by size:
1354 268 10
EcoRV GAT'ATC
Cuts at: 0 1332 1632
Size: 1332 300
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 157 1632
Size: 157 1475
HaeII r_GCGC'y
Cuts at: 0 201 1632
Size: 201 1431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 1447 1479 1632
Size: 1321 32 94 32 153
Fragments arranged by size:
1321 153 94 32 32
HincII GTy'rAC
Cuts at: 0 396 1632
Size: 396 1236
HindIII A'AGCT_T
Cuts at: 0 1118 1310 1632
Size: 1118 192 322
Fragments arranged by size:
1118 322 192
MfeI C'AATT_G
Cuts at: 0 1355 1632
Size: 1355 277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1632
Size: 338 1294
MscI TGG'CCA
Cuts at: 0 883 1632
Size: 883 749
MslI CAynn'nnrTG
Cuts at: 0 1264 1632
Size: 1264 368
MspA1I CmG'CkG
Cuts at: 0 1096 1468 1632
Size: 1096 372 164
Fragments arranged by size:
1096 372 164
NcoI C'CATG_G
Cuts at: 0 107 1632
Size: 107 1525
PpuMI rG'GwC_Cy
Cuts at: 0 786 1632
Size: 786 846
PstI C_TGCA'G
Cuts at: 0 1105 1632
Size: 1105 527
PvuII CAG'CTG
Cuts at: 0 1096 1468 1632
Size: 1096 372 164
Fragments arranged by size:
1096 372 164
SacI G_AGCT'C
Cuts at: 0 1445 1632
Size: 1445 187
SanDI GG'GwC_CC
Cuts at: 0 786 1632
Size: 786 846
ScaI AGT'ACT
Cuts at: 0 693 1632
Size: 693 939
SexAI A'CCwGG_T
Cuts at: 0 1142 1632
Size: 1142 490
SfcI C'TryA_G
Cuts at: 0 1101 1632
Size: 1101 531
SmlI C'TyrA_G
Cuts at: 0 1498 1632
Size: 1498 134
SspI AAT'ATT
Cuts at: 0 972 1176 1632
Size: 972 204 456
Fragments arranged by size:
972 456 204
StyI C'CwwG_G
Cuts at: 0 107 1529 1632
Size: 107 1422 103
Fragments arranged by size:
1422 107 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 991 1632
Size: 991 641
TatI w'GTAC_w
Cuts at: 0 469 691 698 1632
Size: 469 222 7 934
Fragments arranged by size:
934 469 222 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 264 287 296 319 863 895 1632
Size: 264 23 9 23 544 32 737
Fragments arranged by size:
737 544 264 32 23 23 9
XbaI T'CTAG_A
Cuts at: 0 357 1632
Size: 357 1275
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaLI ApoI AseI AvaI
BanI BanII BciVI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI PpuMI PstI PvuII SacI SanDI ScaI SexAI
SfcI SmlI SspI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI AscI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoNI
FseI FspI FspAI HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI
XhoI XmaI XmnI ZraI