Restriction map for
pF1KB5924
(Linear) (Six-base) MAPSORT of: KIBB5924.seq Check: 5778 from: 1 to: 1560
KIBB5924.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AccI GT'mk_AC
Cuts at: 0 716 998 1560
Size: 716 282 562
Fragments arranged by size:
716 562 282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1217 1445 1560
Size: 830 387 228 115
Fragments arranged by size:
830 387 228 115
AflIII A'CryG_T
Cuts at: 0 181 1242 1560
Size: 181 1061 318
Fragments arranged by size:
1061 318 181
AhdI GACnn_n'nnGTC
Cuts at: 0 534 1560
Size: 534 1026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1286 1560
Size: 1286 274
AlwNI CAG_nnn'CTG
Cuts at: 0 273 1423 1560
Size: 273 1150 137
Fragments arranged by size:
1150 273 137
ApoI r'AATT_y
Cuts at: 0 100 463 1038 1144 1276 1560
Size: 100 363 575 106 132 284
Fragments arranged by size:
575 363 284 132 106 100
AvaI C'yCGr_G
Cuts at: 0 1121 1560
Size: 1121 439
BanII G_rGCy'C
Cuts at: 0 557 1128 1560
Size: 557 571 432
Fragments arranged by size:
571 557 432
BbsI GAAGACnn'nnnn_
Cuts at: 0 818 1560
Size: 818 742
BglI GCCn_nnn'nGGC
Cuts at: 0 685 922 1560
Size: 685 237 638
Fragments arranged by size:
685 638 237
BglII A'GATC_T
Cuts at: 0 581 1560
Size: 581 979
BlpI GC'TnA_GC
Cuts at: 0 400 1560
Size: 400 1160
BmrI ACTGGGnnnn_n'
Cuts at: 0 328 941 1560
Size: 328 613 619
Fragments arranged by size:
619 613 328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 764 1560
Size: 764 796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 541 1076 1397 1560
Size: 204 337 535 321 163
Fragments arranged by size:
535 337 321 204 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 1534 1560
Size: 1534 26
BsaBI GATnn'nnATC
Cuts at: 0 749 1560
Size: 749 811
BsaWI w'CCGG_w
Cuts at: 0 458 1560
Size: 458 1102
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 641 1400 1468 1560
Size: 22 619 759 68 92
Fragments arranged by size:
759 619 92 68 22
BsiHKAI G_wGCw'C
Cuts at: 0 557 1560
Size: 557 1003
BsmI GAATG_Cn'
Cuts at: 0 212 1344 1560
Size: 212 1132 216
Fragments arranged by size:
1132 216 212
Bsp1286I G_dGCh'C
Cuts at: 0 557 1128 1560
Size: 557 571 432
Fragments arranged by size:
571 557 432
BspHI T'CATG_A
Cuts at: 0 1235 1560
Size: 1235 325
BsrBI CCG'CTC
Cuts at: 0 57 1560
Size: 57 1503
BsrDI GCAATG_nn'
Cuts at: 0 843 864 1560
Size: 843 21 696
Fragments arranged by size:
843 696 21
BsrFI r'CCGG_y
Cuts at: 0 34 1560
Size: 34 1526
BssHII G'CGCG_C
Cuts at: 0 12 1560
Size: 12 1548
BstAPI GCAn_nnn'nTGC
Cuts at: 0 273 1560
Size: 273 1287
BstYI r'GATC_y
Cuts at: 0 581 1560
Size: 581 979
BstZ17I GTA'TAC
Cuts at: 0 999 1560
Size: 999 561
Bsu36I CC'TnA_GG
Cuts at: 0 64 662 1560
Size: 64 598 898
Fragments arranged by size:
898 598 64
BtgI C'CryG_G
Cuts at: 0 992 1560
Size: 992 568
DraI TTT'AAA
Cuts at: 0 153 1560
Size: 153 1407
DrdI GACnn_nn'nnGTC
Cuts at: 0 1255 1560
Size: 1255 305
EaeI y'GGCC_r
Cuts at: 0 629 1358 1560
Size: 629 729 202
Fragments arranged by size:
729 629 202
EarI CTCTTCn'nnn_
Cuts at: 0 508 706 818 1045 1377 1532 1560
Size: 508 198 112 227 332 155 28
Fragments arranged by size:
508 332 227 198 155 112 28
EcoICRI GAG'CTC
Cuts at: 0 555 1560
Size: 555 1005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 541 830 1076 1217 1397 1445 1560
Size: 204 337 289 246 141 180 48 115
Fragments arranged by size:
337 289 246 204 180 141 115 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 1560
Size: 163 1397
EcoO109I rG'GnC_Cy
Cuts at: 0 60 687 1365 1560
Size: 60 627 678 195
Fragments arranged by size:
678 627 195 60
EcoRI G'AATT_C
Cuts at: 0 1276 1560
Size: 1276 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 520 552 610 638 642 670
Size: 307 32 181 32 58 28 4 28
Cuts at: 670 1560
Size: 890
Fragments arranged by size:
890 307 181 58 32 32 28 28
4
HincII GTy'rAC
Cuts at: 0 846 899 1248 1560
Size: 846 53 349 312
Fragments arranged by size:
846 349 312 53
HpaI GTT'AAC
Cuts at: 0 899 1560
Size: 899 661
MfeI C'AATT_G
Cuts at: 0 541 1560
Size: 541 1019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 829 1560
Size: 703 126 731
Fragments arranged by size:
731 703 126
MslI CAynn'nnrTG
Cuts at: 0 354 836 1560
Size: 354 482 724
Fragments arranged by size:
724 482 354
MspA1I CmG'CkG
Cuts at: 0 570 772 961 1560
Size: 570 202 189 599
Fragments arranged by size:
599 570 202 189
NaeI GCC'GGC
Cuts at: 0 36 1560
Size: 36 1524
NcoI C'CATG_G
Cuts at: 0 992 1560
Size: 992 568
NgoMIV G'CCGG_C
Cuts at: 0 34 1560
Size: 34 1526
NspI r_CATG'y
Cuts at: 0 185 1246 1560
Size: 185 1061 314
Fragments arranged by size:
1061 314 185
PciI A'CATG_T
Cuts at: 0 181 1242 1560
Size: 181 1061 318
Fragments arranged by size:
1061 318 181
PpuMI rG'GwC_Cy
Cuts at: 0 60 1560
Size: 60 1500
PsiI TTA'TAA
Cuts at: 0 163 1560
Size: 163 1397
PstI C_TGCA'G
Cuts at: 0 1347 1560
Size: 1347 213
PvuII CAG'CTG
Cuts at: 0 772 961 1560
Size: 772 189 599
Fragments arranged by size:
772 599 189
SacI G_AGCT'C
Cuts at: 0 557 1560
Size: 557 1003
SapI GCTCTTCn'nnn_
Cuts at: 0 508 818 1560
Size: 508 310 742
Fragments arranged by size:
742 508 310
ScaI AGT'ACT
Cuts at: 0 206 1525 1560
Size: 206 1319 35
Fragments arranged by size:
1319 206 35
SfcI C'TryA_G
Cuts at: 0 1074 1343 1560
Size: 1074 269 217
Fragments arranged by size:
1074 269 217
StyI C'CwwG_G
Cuts at: 0 992 1251 1560
Size: 992 259 309
Fragments arranged by size:
992 309 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 628 1560
Size: 628 932
TatI w'GTAC_w
Cuts at: 0 204 1523 1560
Size: 204 1319 37
Fragments arranged by size:
1319 204 37
XbaI T'CTAG_A
Cuts at: 0 933 1560
Size: 933 627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1368 1560
Size: 1368 192
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AlwNI ApoI AvaI
BanII BbsI BglI BglII BlpI BmrI BplI BpmI
BsaI BsaBI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrDI BsrFI BssHII BstAPI BstYI BstZ17I Bsu36I
BtgI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII HpaI MfeI MmeI MslI
MspA1I NaeI NcoI NgoMIV NspI PciI PpuMI PsiI
PstI PvuII SacI SapI ScaI SfcI StyI TaqII
TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrGI
BssSI BstBI BstEII BstXI BtgZI BtsI ClaI CspCI
DraIII EagI EciI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MluI MscI NarI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XhoI XmaI XmnI
ZraI