Restriction map for
pF1KB5894
(Linear) (Six-base) MAPSORT of: KIBB5894.seq Check: 4228 from: 1 to: 1572
KIBB5894.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1572
Size: 676 896
AflIII A'CryG_T
Cuts at: 0 129 1572
Size: 129 1443
AleI CACnn'nnGTG
Cuts at: 0 49 1572
Size: 49 1523
AlwNI CAG_nnn'CTG
Cuts at: 0 699 1567 1572
Size: 699 868 5
Fragments arranged by size:
868 699 5
ApoI r'AATT_y
Cuts at: 0 625 1492 1546 1572
Size: 625 867 54 26
Fragments arranged by size:
867 625 54 26
AseI AT'TA_AT
Cuts at: 0 818 1572
Size: 818 754
AvrII C'CTAG_G
Cuts at: 0 1551 1572
Size: 1551 21
BanII G_rGCy'C
Cuts at: 0 417 1572
Size: 417 1155
BbvCI CC'TCA_GC
Cuts at: 0 245 1572
Size: 245 1327
BciVI GTATCCnnnnn_n'
Cuts at: 0 312 1572
Size: 312 1260
BclI T'GATC_A
Cuts at: 0 89 1572
Size: 89 1483
BlpI GC'TnA_GC
Cuts at: 0 938 1512 1572
Size: 938 574 60
Fragments arranged by size:
938 574 60
BmgBI CAC'GTC
Cuts at: 0 1294 1572
Size: 1294 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 1572
Size: 29 1543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1572
Size: 1430 142
Bpu10I CC'TnA_GC
Cuts at: 0 245 1572
Size: 245 1327
BsaBI GATnn'nnATC
Cuts at: 0 1207 1572
Size: 1207 365
BsaWI w'CCGG_w
Cuts at: 0 949 1572
Size: 949 623
BseYI C'CCAG_C
Cuts at: 0 54 1572
Size: 54 1518
BsiHKAI G_wGCw'C
Cuts at: 0 417 561 1418 1572
Size: 417 144 857 154
Fragments arranged by size:
857 417 154 144
BsmI GAATG_Cn'
Cuts at: 0 596 1318 1572
Size: 596 722 254
Fragments arranged by size:
722 596 254
Bsp1286I G_dGCh'C
Cuts at: 0 417 561 1418 1572
Size: 417 144 857 154
Fragments arranged by size:
857 417 154 144
BspEI T'CCGG_A
Cuts at: 0 949 1572
Size: 949 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1342 1572
Size: 1342 230
BsrBI CCG'CTC
Cuts at: 0 547 1572
Size: 547 1025
BsrDI GCAATG_nn'
Cuts at: 0 1367 1572
Size: 1367 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 660 1315 1572
Size: 660 655 257
Fragments arranged by size:
660 655 257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 239 1572
Size: 239 1333
BtsI GCAGTG_nn'
Cuts at: 0 44 1055 1572
Size: 44 1011 517
Fragments arranged by size:
1011 517 44
DraI TTT'AAA
Cuts at: 0 1446 1572
Size: 1446 126
EaeI y'GGCC_r
Cuts at: 0 581 1572
Size: 581 991
EarI CTCTTCn'nnn_
Cuts at: 0 245 886 1498 1572
Size: 245 641 612 74
Fragments arranged by size:
641 612 245 74
EcoICRI GAG'CTC
Cuts at: 0 415 1572
Size: 415 1157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1430 1572
Size: 676 754 142
Fragments arranged by size:
754 676 142
EcoO109I rG'GnC_Cy
Cuts at: 0 23 1572
Size: 23 1549
EcoRI G'AATT_C
Cuts at: 0 1492 1572
Size: 1492 80
HaeII r_GCGC'y
Cuts at: 0 1040 1572
Size: 1040 532
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 603 635 1085 1117 1572
Size: 603 32 450 32 455
Fragments arranged by size:
603 455 450 32 32
HindIII A'AGCT_T
Cuts at: 0 774 1572
Size: 774 798
MfeI C'AATT_G
Cuts at: 0 1326 1572
Size: 1326 246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 565 1572
Size: 390 175 1007
Fragments arranged by size:
1007 390 175
MscI TGG'CCA
Cuts at: 0 583 1572
Size: 583 989
MslI CAynn'nnrTG
Cuts at: 0 49 85 1144 1150 1572
Size: 49 36 1059 6 422
Fragments arranged by size:
1059 422 49 36 6
MspA1I CmG'CkG
Cuts at: 0 265 579 1572
Size: 265 314 993
Fragments arranged by size:
993 314 265
NspI r_CATG'y
Cuts at: 0 133 934 1572
Size: 133 801 638
Fragments arranged by size:
801 638 133
PciI A'CATG_T
Cuts at: 0 129 1572
Size: 129 1443
PflMI CCAn_nnn'nTGG
Cuts at: 0 287 1572
Size: 287 1285
PpuMI rG'GwC_Cy
Cuts at: 0 23 1572
Size: 23 1549
PsiI TTA'TAA
Cuts at: 0 460 1572
Size: 460 1112
PstI C_TGCA'G
Cuts at: 0 1491 1572
Size: 1491 81
PvuII CAG'CTG
Cuts at: 0 265 579 1572
Size: 265 314 993
Fragments arranged by size:
993 314 265
SacI G_AGCT'C
Cuts at: 0 417 1572
Size: 417 1155
SanDI GG'GwC_CC
Cuts at: 0 23 1572
Size: 23 1549
SapI GCTCTTCn'nnn_
Cuts at: 0 886 1572
Size: 886 686
SfcI C'TryA_G
Cuts at: 0 123 1487 1535 1559 1572
Size: 123 1364 48 24 13
Fragments arranged by size:
1364 123 48 24 13
StuI AGG'CCT
Cuts at: 0 509 1572
Size: 509 1063
StyI C'CwwG_G
Cuts at: 0 238 1551 1572
Size: 238 1313 21
Fragments arranged by size:
1313 238 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 170 335 1572
Size: 127 43 165 1237
Fragments arranged by size:
1237 165 127 43
TatI w'GTAC_w
Cuts at: 0 187 1364 1572
Size: 187 1177 208
Fragments arranged by size:
1177 208 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1376 1408 1572
Size: 1376 32 164
Fragments arranged by size:
1376 164 32
Tth111I GACn'n_nGTC
Cuts at: 0 1292 1572
Size: 1292 280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1504 1572
Size: 1504 68
XmnI GAAnn'nnTTC
Cuts at: 0 1174 1572
Size: 1174 398
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AseI AvrII BanII
BbvCI BciVI BclI BlpI BmgBI BmrI BpmI Bpu10I
BsaBI BsaWI BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BstAPI BstXI BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NspI PciI PflMI PpuMI
PsiI PstI PvuII SacI SanDI SapI SfcI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
BaeI BamHI BanI BbeI BbsI BcgI BfrBI BglI
BglII Bme1580I BmtI BplI BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI ZraI