Restriction map for
pF1KB5886
(Linear) (Six-base) MAPSORT of: KIBB5886.seq Check: 4214 from: 1 to: 1491
KIBB5886.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 500 1071 1491
Size: 500 571 420
Fragments arranged by size:
571 500 420
AccI GT'mk_AC
Cuts at: 0 374 1491
Size: 374 1117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 829 1491
Size: 473 356 662
Fragments arranged by size:
662 473 356
AlwNI CAG_nnn'CTG
Cuts at: 0 831 915 1491
Size: 831 84 576
Fragments arranged by size:
831 576 84
ApoI r'AATT_y
Cuts at: 0 442 733 1491
Size: 442 291 758
Fragments arranged by size:
758 442 291
AvaI C'yCGr_G
Cuts at: 0 49 350 620 1491
Size: 49 301 270 871
Fragments arranged by size:
871 301 270 49
AvrII C'CTAG_G
Cuts at: 0 774 1491
Size: 774 717
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1235 1268 1491
Size: 1235 33 223
Fragments arranged by size:
1235 223 33
BamHI G'GATC_C
Cuts at: 0 615 1491
Size: 615 876
BanII G_rGCy'C
Cuts at: 0 117 176 371 467 535 1043 1070 1131
Size: 117 59 195 96 68 508 27 61
Cuts at: 1131 1316 1491
Size: 185 175
Fragments arranged by size:
508 195 185 175 117 96 68 61
59 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 817 870 1491
Size: 817 53 621
Fragments arranged by size:
817 621 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1069 1103 1491
Size: 1069 34 388
Fragments arranged by size:
1069 388 34
BglI GCCn_nnn'nGGC
Cuts at: 0 483 892 1438 1491
Size: 483 409 546 53
Fragments arranged by size:
546 483 409 53
BlpI GC'TnA_GC
Cuts at: 0 229 758 1491
Size: 229 529 733
Fragments arranged by size:
733 529 229
Bme1580I G_kGCm'C
Cuts at: 0 68 1491
Size: 68 1423
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 422 1041 1491
Size: 240 182 619 450
Fragments arranged by size:
619 450 240 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 787 937 1411 1432 1491
Size: 616 171 150 474 21 59
Fragments arranged by size:
616 474 171 150 59 21
Bpu10I CC'TnA_GC
Cuts at: 0 1159 1491
Size: 1159 332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 493 622 1258 1491
Size: 274 219 129 636 233
Fragments arranged by size:
636 274 233 219 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 682 1491
Size: 682 809
BsaAI yAC'GTr
Cuts at: 0 945 1491
Size: 945 546
BsaWI w'CCGG_w
Cuts at: 0 413 719 1491
Size: 413 306 772
Fragments arranged by size:
772 413 306
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 616 646 985 1015 1207 1237
Size: 76 30 510 30 339 30 192 30
Cuts at: 1237 1491
Size: 254
Fragments arranged by size:
510 339 254 192 76 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 129 1408 1491
Size: 33 96 1279 83
Fragments arranged by size:
1279 96 83 33
BseYI C'CCAG_C
Cuts at: 0 887 1491
Size: 887 604
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 634 838 928 966 1491
Size: 202 432 204 90 38 525
Fragments arranged by size:
525 432 204 202 90 38
BsiHKAI G_wGCw'C
Cuts at: 0 176 349 743 821 944 1235 1274 1316
Size: 176 173 394 78 123 291 39 42
Cuts at: 1316 1491
Size: 175
Fragments arranged by size:
394 291 176 175 173 123 78 42
39
Bsp1286I G_dGCh'C
Cuts at: 0 68 117 176 349 371 467 535 743
Size: 68 49 59 173 22 96 68 208
Cuts at: 743 821 944 1043 1070 1131 1235 1274 1316
Size: 78 123 99 27 61 104 39 42
Cuts at: 1316 1491
Size: 175
Fragments arranged by size:
208 175 173 123 104 99 96 78
68 68 61 59 49 42 39 27
22
BspEI T'CCGG_A
Cuts at: 0 413 1491
Size: 413 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 500 988 1071 1491
Size: 500 488 83 420
Fragments arranged by size:
500 488 420 83
BsrBI CCG'CTC
Cuts at: 0 128 1125 1491
Size: 128 997 366
Fragments arranged by size:
997 366 128
BsrFI r'CCGG_y
Cuts at: 0 204 1409 1491
Size: 204 1205 82
Fragments arranged by size:
1205 204 82
BssSI C'ACGA_G
Cuts at: 0 220 1491
Size: 220 1271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 225 701 1491
Size: 225 476 790
Fragments arranged by size:
790 476 225
BstYI r'GATC_y
Cuts at: 0 615 770 1058 1491
Size: 615 155 288 433
Fragments arranged by size:
615 433 288 155
BtgI C'CryG_G
Cuts at: 0 551 1491
Size: 551 940
DraIII CAC_nnn'GTG
Cuts at: 0 1188 1491
Size: 1188 303
EaeI y'GGCC_r
Cuts at: 0 281 484 554 970 1469 1491
Size: 281 203 70 416 499 22
Fragments arranged by size:
499 416 281 203 70 22
EarI CTCTTCn'nnn_
Cuts at: 0 449 1013 1491
Size: 449 564 478
Fragments arranged by size:
564 478 449
EcoICRI GAG'CTC
Cuts at: 0 174 1314 1491
Size: 174 1140 177
Fragments arranged by size:
1140 177 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 616 787 829 937 1411 1432 1491
Size: 473 143 171 42 108 474 21 59
Fragments arranged by size:
474 473 171 143 108 59 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 919 1168 1491
Size: 919 249 323
Fragments arranged by size:
919 323 249
EcoO109I rG'GnC_Cy
Cuts at: 0 1208 1421 1440 1491
Size: 1208 213 19 51
Fragments arranged by size:
1208 213 51 19
EcoRI G'AATT_C
Cuts at: 0 733 1491
Size: 733 758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1042 1491
Size: 1042 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1491
Size: 76 32 1383
Fragments arranged by size:
1383 76 32
MscI TGG'CCA
Cuts at: 0 486 556 972 1491
Size: 486 70 416 519
Fragments arranged by size:
519 486 416 70
MslI CAynn'nnrTG
Cuts at: 0 391 746 1288 1491
Size: 391 355 542 203
Fragments arranged by size:
542 391 355 203
MspA1I CmG'CkG
Cuts at: 0 183 498 891 1452 1491
Size: 183 315 393 561 39
Fragments arranged by size:
561 393 315 183 39
NaeI GCC'GGC
Cuts at: 0 206 1411 1491
Size: 206 1205 80
Fragments arranged by size:
1205 206 80
NgoMIV G'CCGG_C
Cuts at: 0 204 1409 1491
Size: 204 1205 82
Fragments arranged by size:
1205 204 82
PasI CC'CwG_GG
Cuts at: 0 1425 1491
Size: 1425 66
PfoI T'CCnGG_A
Cuts at: 0 593 914 1061 1491
Size: 593 321 147 430
Fragments arranged by size:
593 430 321 147
PmlI CAC'GTG
Cuts at: 0 945 1491
Size: 945 546
PpuMI rG'GwC_Cy
Cuts at: 0 1208 1421 1491
Size: 1208 213 70
Fragments arranged by size:
1208 213 70
PstI C_TGCA'G
Cuts at: 0 497 1491
Size: 497 994
PvuII CAG'CTG
Cuts at: 0 498 891 1491
Size: 498 393 600
Fragments arranged by size:
600 498 393
SacI G_AGCT'C
Cuts at: 0 176 1316 1491
Size: 176 1140 175
Fragments arranged by size:
1140 176 175
SanDI GG'GwC_CC
Cuts at: 0 1208 1421 1491
Size: 1208 213 70
Fragments arranged by size:
1208 213 70
SfcI C'TryA_G
Cuts at: 0 493 1414 1491
Size: 493 921 77
Fragments arranged by size:
921 493 77
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1438 1491
Size: 1438 53
SmaI CCC'GGG
Cuts at: 0 51 352 622 1491
Size: 51 301 270 869
Fragments arranged by size:
869 301 270 51
SmlI C'TyrA_G
Cuts at: 0 289 472 637 1273 1491
Size: 289 183 165 636 218
Fragments arranged by size:
636 289 218 183 165
StyI C'CwwG_G
Cuts at: 0 774 1226 1433 1491
Size: 774 452 207 58
Fragments arranged by size:
774 452 207 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1000 1491
Size: 1000 491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 1491
Size: 564 927
XmaI C'CCGG_G
Cuts at: 0 49 350 620 1491
Size: 49 301 270 871
Fragments arranged by size:
871 301 270 49
XmnI GAAnn'nnTTC
Cuts at: 0 409 1022 1491
Size: 409 613 469
Fragments arranged by size:
613 469 409
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AvaI AvrII BaeI
BamHI BanII BbsI BcgI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstAPI BstYI BtgI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MscI MslI
MspA1I NaeI NgoMIV PasI PfoI PmlI PpuMI PstI
PvuII SacI SanDI SfcI SfiI SmaI SmlI StyI
TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BanI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI
BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EciI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI ZraI