Restriction map for
pF1KB5884
(Linear) (Six-base) MAPSORT of: KIBB5884.seq Check: 6591 from: 1 to: 1488
KIBB5884.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 0 1001 1488
Size: 1001 487
Acc65I G'GTAC_C
Cuts at: 0 555 1488
Size: 555 933
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 1488
Size: 1020 468
AgeI A'CCGG_T
Cuts at: 0 281 1488
Size: 281 1207
AhdI GACnn_n'nnGTC
Cuts at: 0 921 1302 1488
Size: 921 381 186
Fragments arranged by size:
921 381 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 1488
Size: 796 692
ApaI G_GGCC'C
Cuts at: 0 137 934 1034 1282 1488
Size: 137 797 100 248 206
Fragments arranged by size:
797 248 206 137 100
ApaLI G'TGCA_C
Cuts at: 0 304 1488
Size: 304 1184
AvaI C'yCGr_G
Cuts at: 0 130 452 844 934 1052 1466 1488
Size: 130 322 392 90 118 414 22
Fragments arranged by size:
414 392 322 130 118 90 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1197 1230 1488
Size: 1197 33 258
Fragments arranged by size:
1197 258 33
BanI G'GyrC_C
Cuts at: 0 241 322 481 555 805 1020 1488
Size: 241 81 159 74 250 215 468
Fragments arranged by size:
468 250 241 215 159 81 74
BanII G_rGCy'C
Cuts at: 0 10 137 229 766 934 1034 1159 1200
Size: 10 127 92 537 168 100 125 41
Cuts at: 1200 1262 1282 1488
Size: 62 20 206
Fragments arranged by size:
537 206 168 127 125 100 92 62
41 20 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 1488
Size: 321 1167
BbvCI CC'TCA_GC
Cuts at: 0 202 1488
Size: 202 1286
BciVI GTATCCnnnnn_n'
Cuts at: 0 1225 1488
Size: 1225 263
BglI GCCn_nnn'nGGC
Cuts at: 0 132 770 1029 1286 1488
Size: 132 638 259 257 202
Fragments arranged by size:
638 259 257 202 132
BglII A'GATC_T
Cuts at: 0 602 1488
Size: 602 886
BlpI GC'TnA_GC
Cuts at: 0 31 166 790 1488
Size: 31 135 624 698
Fragments arranged by size:
698 624 135 31
Bme1580I G_kGCm'C
Cuts at: 0 110 128 137 214 246 308 325 683
Size: 110 18 9 77 32 62 17 358
Cuts at: 683 808 934 1034 1282 1430 1488
Size: 125 126 100 248 148 58
Fragments arranged by size:
358 248 148 126 125 110 100 77
62 58 32 18 17 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 504 778 1177 1488
Size: 504 274 399 311
Fragments arranged by size:
504 399 311 274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 825 1488
Size: 825 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 989 1438 1488
Size: 931 58 449 50
Fragments arranged by size:
931 449 58 50
Bpu10I CC'TnA_GC
Cuts at: 0 202 693 1222 1488
Size: 202 491 529 266
Fragments arranged by size:
529 491 266 202
BsaAI yAC'GTr
Cuts at: 0 252 1163 1488
Size: 252 911 325
Fragments arranged by size:
911 325 252
BsaHI Gr'CG_yC
Cuts at: 0 428 1303 1488
Size: 428 875 185
Fragments arranged by size:
875 428 185
BsaWI w'CCGG_w
Cuts at: 0 281 1488
Size: 281 1207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 662 692 1004 1034 1488
Size: 268 30 364 30 312 30 454
Fragments arranged by size:
454 364 312 268 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 481 949 1003 1488
Size: 219 262 468 54 485
Fragments arranged by size:
485 468 262 219 54
BseYI C'CCAG_C
Cuts at: 0 255 351 885 1282 1488
Size: 255 96 534 397 206
Fragments arranged by size:
534 397 255 206 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 394 508 550 802 1488
Size: 232 162 114 42 252 686
Fragments arranged by size:
686 252 232 162 114 42
BsiEI CG_ry'CG
Cuts at: 0 654 1488
Size: 654 834
BsiHKAI G_wGCw'C
Cuts at: 0 10 71 281 308 1488
Size: 10 61 210 27 1180
Fragments arranged by size:
1180 210 61 27 10
Bsp1286I G_dGCh'C
Cuts at: 0 10 71 110 128 137 214 229 246
Size: 10 61 39 18 9 77 15 17
Cuts at: 246 281 308 325 683 766 808 934 1034
Size: 35 27 17 358 83 42 126 100
Cuts at: 1034 1159 1200 1262 1282 1430 1488
Size: 125 41 62 20 148 58
Fragments arranged by size:
358 148 126 125 100 83 77 62
61 58 42 41 39 35 27 20
18 17 17 15 10 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 695 1488
Size: 695 793
BsrBI CCG'CTC
Cuts at: 0 78 1358 1488
Size: 78 1280 130
Fragments arranged by size:
1280 130 78
BsrDI GCAATG_nn'
Cuts at: 0 759 1488
Size: 759 729
BsrFI r'CCGG_y
Cuts at: 0 281 430 733 1314 1488
Size: 281 149 303 581 174
Fragments arranged by size:
581 303 281 174 149
BsrGI T'GTAC_A
Cuts at: 0 1393 1488
Size: 1393 95
BssHII G'CGCG_C
Cuts at: 0 400 1488
Size: 400 1088
BssSI C'ACGA_G
Cuts at: 0 438 676 1488
Size: 438 238 812
Fragments arranged by size:
812 438 238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 605 1488
Size: 605 883
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 1024 1488
Size: 144 880 464
Fragments arranged by size:
880 464 144
BstYI r'GATC_y
Cuts at: 0 602 1488
Size: 602 886
Bsu36I CC'TnA_GG
Cuts at: 0 365 540 1322 1488
Size: 365 175 782 166
Fragments arranged by size:
782 365 175 166
BtgI C'CryG_G
Cuts at: 0 383 522 648 907 1008 1488
Size: 383 139 126 259 101 480
Fragments arranged by size:
480 383 259 139 126 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 656 1488
Size: 327 329 832
Fragments arranged by size:
832 329 327
DraIII CAC_nnn'GTG
Cuts at: 0 528 1185 1488
Size: 528 657 303
Fragments arranged by size:
657 528 303
EaeI y'GGCC_r
Cuts at: 0 218 416 519 651 1488
Size: 218 198 103 132 837
Fragments arranged by size:
837 218 198 132 103
EagI C'GGCC_G
Cuts at: 0 651 1488
Size: 651 837
EarI CTCTTCn'nnn_
Cuts at: 0 76 340 1488
Size: 76 264 1148
Fragments arranged by size:
1148 264 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 289 1207 1488
Size: 289 918 281
Fragments arranged by size:
918 289 281
EcoICRI GAG'CTC
Cuts at: 0 8 1488
Size: 8 1480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 989 1020 1438 1488
Size: 931 58 31 418 50
Fragments arranged by size:
931 418 58 50 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 21 463 1408 1488
Size: 21 442 945 80
Fragments arranged by size:
945 442 80 21
EcoO109I rG'GnC_Cy
Cuts at: 0 42 456 514 931 1031 1109 1278 1279
Size: 42 414 58 417 100 78 169 1
Cuts at: 1279 1329 1367 1413 1488
Size: 50 38 46 75
Fragments arranged by size:
417 414 169 100 78 75 58 50
46 42 38 1
HaeII r_GCGC'y
Cuts at: 0 452 666 790 1488
Size: 452 214 124 698
Fragments arranged by size:
698 452 214 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 603 635 907 939 1093 1125
Size: 268 32 303 32 272 32 154 32
Cuts at: 1125 1288 1320 1488
Size: 163 32 168
Fragments arranged by size:
303 272 268 168 163 154 32 32
32 32 32
KpnI G_GTAC'C
Cuts at: 0 559 1488
Size: 559 929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1488
Size: 1319 169
MscI TGG'CCA
Cuts at: 0 418 1488
Size: 418 1070
MslI CAynn'nnrTG
Cuts at: 0 346 1488
Size: 346 1142
MspA1I CmG'CkG
Cuts at: 0 162 534 708 1236 1250 1286 1488
Size: 162 372 174 528 14 36 202
Fragments arranged by size:
528 372 202 174 162 36 14
NaeI GCC'GGC
Cuts at: 0 432 735 1316 1488
Size: 432 303 581 172
Fragments arranged by size:
581 432 303 172
NgoMIV G'CCGG_C
Cuts at: 0 430 733 1314 1488
Size: 430 303 581 174
Fragments arranged by size:
581 430 303 174
PasI CC'CwG_GG
Cuts at: 0 1262 1488
Size: 1262 226
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 1185 1488
Size: 195 990 303
Fragments arranged by size:
990 303 195
PfoI T'CCnGG_A
Cuts at: 0 966 1111 1415 1488
Size: 966 145 304 73
Fragments arranged by size:
966 304 145 73
PmlI CAC'GTG
Cuts at: 0 252 1163 1488
Size: 252 911 325
Fragments arranged by size:
911 325 252
PpuMI rG'GwC_Cy
Cuts at: 0 42 456 514 1109 1367 1413 1488
Size: 42 414 58 595 258 46 75
Fragments arranged by size:
595 414 258 75 58 46 42
PspOMI G'GGCC_C
Cuts at: 0 133 930 1030 1278 1488
Size: 133 797 100 248 210
Fragments arranged by size:
797 248 210 133 100
PstI C_TGCA'G
Cuts at: 0 15 1049 1488
Size: 15 1034 439
Fragments arranged by size:
1034 439 15
PvuII CAG'CTG
Cuts at: 0 534 708 1250 1488
Size: 534 174 542 238
Fragments arranged by size:
542 534 238 174
SacI G_AGCT'C
Cuts at: 0 10 1488
Size: 10 1478
SanDI GG'GwC_CC
Cuts at: 0 42 514 1488
Size: 42 472 974
Fragments arranged by size:
974 472 42
SapI GCTCTTCn'nnn_
Cuts at: 0 76 1488
Size: 76 1412
SfcI C'TryA_G
Cuts at: 0 11 636 998 1045 1488
Size: 11 625 362 47 443
Fragments arranged by size:
625 443 362 47 11
SmaI CCC'GGG
Cuts at: 0 132 454 936 1488
Size: 132 322 482 552
Fragments arranged by size:
552 482 322 132
StuI AGG'CCT
Cuts at: 0 674 1086 1488
Size: 674 412 402
Fragments arranged by size:
674 412 402
StyI C'CwwG_G
Cuts at: 0 22 1452 1488
Size: 22 1430 36
Fragments arranged by size:
1430 36 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 952 1196 1254 1488
Size: 952 244 58 234
Fragments arranged by size:
952 244 234 58
TatI w'GTAC_w
Cuts at: 0 1172 1393 1488
Size: 1172 221 95
Fragments arranged by size:
1172 221 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 1004 1036 1488
Size: 662 32 310 32 452
Fragments arranged by size:
662 452 310 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 507 1024 1488
Size: 507 517 464
Fragments arranged by size:
517 507 464
XmaI C'CCGG_G
Cuts at: 0 130 452 934 1488
Size: 130 322 482 554
Fragments arranged by size:
554 482 322 130
Enzymes that do cut:
AccI Acc65I AcuI AgeI AhdI AlfI ApaI ApaLI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SfcI SmaI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AleI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BclI BfrBI BmgBI BmtI BpuEI BsaI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BstBI BstEII BstZ17I
BtsI ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI