Restriction map for
pF1KB5867
(Linear) (Six-base) MAPSORT of: KIBB5867.seq Check: 1365 from: 1 to: 1386
KIBB5867.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 804 1386
Size: 804 582
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1283 1386
Size: 497 786 103
Fragments arranged by size:
786 497 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1105 1386
Size: 1105 281
ApoI r'AATT_y
Cuts at: 0 514 1225 1386
Size: 514 711 161
Fragments arranged by size:
711 514 161
BanI G'GyrC_C
Cuts at: 0 1258 1386
Size: 1258 128
BbeI G_GCGC'C
Cuts at: 0 1262 1386
Size: 1262 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 557 839 944 1386
Size: 97 460 282 105 442
Fragments arranged by size:
460 442 282 105 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1096 1130 1386
Size: 1096 34 256
Fragments arranged by size:
1096 256 34
BfrBI ATG'CAT
Cuts at: 0 628 1386
Size: 628 758
BglII A'GATC_T
Cuts at: 0 660 1386
Size: 660 726
Bme1580I G_kGCm'C
Cuts at: 0 429 1386
Size: 429 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1386
Size: 120 1266
Bpu10I CC'TnA_GC
Cuts at: 0 23 179 1386
Size: 23 156 1207
Fragments arranged by size:
1207 156 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 749 1386
Size: 749 637
BsaAI yAC'GTr
Cuts at: 0 1350 1386
Size: 1350 36
BsaBI GATnn'nnATC
Cuts at: 0 704 1386
Size: 704 682
BsaHI Gr'CG_yC
Cuts at: 0 1259 1386
Size: 1259 127
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 701 1386
Size: 701 685
BseYI C'CCAG_C
Cuts at: 0 642 1386
Size: 642 744
BsiHKAI G_wGCw'C
Cuts at: 0 925 1386
Size: 925 461
BsmI GAATG_Cn'
Cuts at: 0 1133 1386
Size: 1133 253
Bsp1286I G_dGCh'C
Cuts at: 0 429 925 1386
Size: 429 496 461
Fragments arranged by size:
496 461 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1287 1386
Size: 1287 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 16 1386
Size: 16 1370
BstYI r'GATC_y
Cuts at: 0 660 699 1111 1335 1386
Size: 660 39 412 224 51
Fragments arranged by size:
660 412 224 51 39
BstZ17I GTA'TAC
Cuts at: 0 805 1386
Size: 805 581
Bsu36I CC'TnA_GG
Cuts at: 0 842 1386
Size: 842 544
BtgI C'CryG_G
Cuts at: 0 74 152 603 1386
Size: 74 78 451 783
Fragments arranged by size:
783 451 78 74
BtsI GCAGTG_nn'
Cuts at: 0 117 234 1087 1386
Size: 117 117 853 299
Fragments arranged by size:
853 299 117 117
DraI TTT'AAA
Cuts at: 0 120 134 1386
Size: 120 14 1252
Fragments arranged by size:
1252 120 14
EaeI y'GGCC_r
Cuts at: 0 66 231 1344 1386
Size: 66 165 1113 42
Fragments arranged by size:
1113 165 66 42
EarI CTCTTCn'nnn_
Cuts at: 0 177 287 314 461 1386
Size: 177 110 27 147 925
Fragments arranged by size:
925 177 147 110 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 497 1283 1386
Size: 120 377 786 103
Fragments arranged by size:
786 377 120 103
EcoRI G'AATT_C
Cuts at: 0 514 1386
Size: 514 872
EcoRV GAT'ATC
Cuts at: 0 791 1386
Size: 791 595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 288 986 1386
Size: 288 698 400
Fragments arranged by size:
698 400 288
HaeII r_GCGC'y
Cuts at: 0 189 1262 1386
Size: 189 1073 124
Fragments arranged by size:
1073 189 124
HindIII A'AGCT_T
Cuts at: 0 597 1386
Size: 597 789
KasI G'GCGC_C
Cuts at: 0 1258 1386
Size: 1258 128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1386
Size: 818 568
MscI TGG'CCA
Cuts at: 0 233 1346 1386
Size: 233 1113 40
Fragments arranged by size:
1113 233 40
MspA1I CmG'CkG
Cuts at: 0 646 760 1386
Size: 646 114 626
Fragments arranged by size:
646 626 114
NarI GG'CG_CC
Cuts at: 0 1259 1386
Size: 1259 127
NcoI C'CATG_G
Cuts at: 0 74 152 1386
Size: 74 78 1234
Fragments arranged by size:
1234 78 74
NsiI A_TGCA'T
Cuts at: 0 630 1386
Size: 630 756
NspI r_CATG'y
Cuts at: 0 208 1386
Size: 208 1178
PmlI CAC'GTG
Cuts at: 0 1350 1386
Size: 1350 36
PstI C_TGCA'G
Cuts at: 0 657 1386
Size: 657 729
PvuII CAG'CTG
Cuts at: 0 646 760 1386
Size: 646 114 626
Fragments arranged by size:
646 626 114
SfcI C'TryA_G
Cuts at: 0 159 653 927 1386
Size: 159 494 274 459
Fragments arranged by size:
494 459 274 159
SfoI GGC'GCC
Cuts at: 0 1260 1386
Size: 1260 126
SphI G_CATG'C
Cuts at: 0 208 1386
Size: 208 1178
SspI AAT'ATT
Cuts at: 0 138 1386
Size: 138 1248
StuI AGG'CCT
Cuts at: 0 453 945 1386
Size: 453 492 441
Fragments arranged by size:
492 453 441
StyI C'CwwG_G
Cuts at: 0 74 152 1386
Size: 74 78 1234
Fragments arranged by size:
1234 78 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 408 596 1330 1386
Size: 408 188 734 56
Fragments arranged by size:
734 408 188 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 984 1016 1386
Size: 984 32 370
Fragments arranged by size:
984 370 32
Enzymes that do cut:
AccI AcuI AlfI ApoI BanI BbeI BbsI BcgI
BfrBI BglII Bme1580I BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BstAPI
BstYI BstZ17I Bsu36I BtgI BtsI DraI EaeI EarI
Eco57MI EcoRI EcoRV FalI HaeII HindIII KasI MmeI
MscI MspA1I NarI NcoI NsiI NspI PmlI PstI
PvuII SfcI SfoI SphI SspI StuI StyI TaqII
TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BciVI
BclI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I FseI FspI FspAI Hin4I HincII
HpaI KpnI MfeI MluI MslI NaeI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI