Restriction map for
pF1KB5854
(Linear) (Six-base) MAPSORT of: KIBB5854.seq Check: 8014 from: 1 to: 1464
KIBB5854.orf
With 182 enzymes: SgfI *
September 25, 2012 15:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 334 1464
Size: 334 1130
AflIII A'CryG_T
Cuts at: 0 1202 1464
Size: 1202 262
AgeI A'CCGG_T
Cuts at: 0 935 1464
Size: 935 529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1253 1464
Size: 1253 211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 1464
Size: 55 32 1377
Fragments arranged by size:
1377 55 32
AlwNI CAG_nnn'CTG
Cuts at: 0 528 750 1464
Size: 528 222 714
Fragments arranged by size:
714 528 222
ApaI G_GGCC'C
Cuts at: 0 506 1264 1464
Size: 506 758 200
Fragments arranged by size:
758 506 200
ApaLI G'TGCA_C
Cuts at: 0 904 1464
Size: 904 560
ApoI r'AATT_y
Cuts at: 0 235 1464
Size: 235 1229
AvaI C'yCGr_G
Cuts at: 0 58 313 1030 1464
Size: 58 255 717 434
Fragments arranged by size:
717 434 255 58
BanI G'GyrC_C
Cuts at: 0 5 1014 1143 1464
Size: 5 1009 129 321
Fragments arranged by size:
1009 321 129 5
BanII G_rGCy'C
Cuts at: 0 506 1264 1464
Size: 506 758 200
Fragments arranged by size:
758 506 200
BbeI G_GCGC'C
Cuts at: 0 9 1018 1464
Size: 9 1009 446
Fragments arranged by size:
1009 446 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1075 1464
Size: 1075 389
BglI GCCn_nnn'nGGC
Cuts at: 0 40 1464
Size: 40 1424
Bme1580I G_kGCm'C
Cuts at: 0 506 908 1148 1264 1332 1464
Size: 506 402 240 116 68 132
Fragments arranged by size:
506 402 240 132 116 68
BmgBI CAC'GTC
Cuts at: 0 1269 1464
Size: 1269 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 1050 1464
Size: 1050 414
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 1464
Size: 201 1263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1464
Size: 1159 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1114 1360 1464
Size: 967 147 246 104
Fragments arranged by size:
967 246 147 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 265 943 1464
Size: 172 93 678 521
Fragments arranged by size:
678 521 172 93
BsaAI yAC'GTr
Cuts at: 0 1152 1464
Size: 1152 312
BsaHI Gr'CG_yC
Cuts at: 0 6 1015 1464
Size: 6 1009 449
Fragments arranged by size:
1009 449 6
BsaWI w'CCGG_w
Cuts at: 0 869 935 1464
Size: 869 66 529
Fragments arranged by size:
869 529 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1437 1464
Size: 1437 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 901 1464
Size: 901 563
BseYI C'CCAG_C
Cuts at: 0 1313 1464
Size: 1313 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1464
Size: 312 1152
BsiHKAI G_wGCw'C
Cuts at: 0 448 908 977 1464
Size: 448 460 69 487
Fragments arranged by size:
487 460 448 69
BsmI GAATG_Cn'
Cuts at: 0 491 724 1464
Size: 491 233 740
Fragments arranged by size:
740 491 233
Bsp1286I G_dGCh'C
Cuts at: 0 448 506 908 977 1148 1264 1332 1464
Size: 448 58 402 69 171 116 68 132
Fragments arranged by size:
448 402 171 132 116 69 68 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 190 334 656 1464
Size: 190 144 322 808
Fragments arranged by size:
808 322 190 144
BsrBI CCG'CTC
Cuts at: 0 1085 1464
Size: 1085 379
BsrFI r'CCGG_y
Cuts at: 0 935 1112 1213 1464
Size: 935 177 101 251
Fragments arranged by size:
935 251 177 101
BssSI C'ACGA_G
Cuts at: 0 114 421 907 1102 1464
Size: 114 307 486 195 362
Fragments arranged by size:
486 362 307 195 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 750 1166 1464
Size: 488 262 416 298
Fragments arranged by size:
488 416 298 262
BstBI TT'CG_AA
Cuts at: 0 347 1464
Size: 347 1117
BstEII G'GTnAC_C
Cuts at: 0 1362 1464
Size: 1362 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 1464
Size: 705 759
BstYI r'GATC_y
Cuts at: 0 1349 1464
Size: 1349 115
BtgI C'CryG_G
Cuts at: 0 456 1464
Size: 456 1008
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 951 1464
Size: 951 513
BtsI GCAGTG_nn'
Cuts at: 0 10 1464
Size: 10 1454
DraIII CAC_nnn'GTG
Cuts at: 0 912 1464
Size: 912 552
EarI CTCTTCn'nnn_
Cuts at: 0 131 170 188 535 1464
Size: 131 39 18 347 929
Fragments arranged by size:
929 347 131 39 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1464
Size: 1159 305
EcoO109I rG'GnC_Cy
Cuts at: 0 502 503 579 747 1260 1464
Size: 502 1 76 168 513 204
Fragments arranged by size:
513 502 204 168 76 1
EcoRI G'AATT_C
Cuts at: 0 235 1464
Size: 235 1229
HaeII r_GCGC'y
Cuts at: 0 9 1018 1464
Size: 9 1009 446
Fragments arranged by size:
1009 446 9
KasI G'GCGC_C
Cuts at: 0 5 1014 1464
Size: 5 1009 450
Fragments arranged by size:
1009 450 5
MslI CAynn'nnrTG
Cuts at: 0 970 1009 1057 1464
Size: 970 39 48 407
Fragments arranged by size:
970 407 48 39
MspA1I CmG'CkG
Cuts at: 0 458 572 1166 1256 1313 1464
Size: 458 114 594 90 57 151
Fragments arranged by size:
594 458 151 114 90 57
NaeI GCC'GGC
Cuts at: 0 1114 1215 1464
Size: 1114 101 249
Fragments arranged by size:
1114 249 101
NarI GG'CG_CC
Cuts at: 0 6 1015 1464
Size: 6 1009 449
Fragments arranged by size:
1009 449 6
NdeI CA'TA_TG
Cuts at: 0 606 1464
Size: 606 858
NgoMIV G'CCGG_C
Cuts at: 0 1112 1213 1464
Size: 1112 101 251
Fragments arranged by size:
1112 251 101
NspI r_CATG'y
Cuts at: 0 1206 1464
Size: 1206 258
PciI A'CATG_T
Cuts at: 0 1202 1464
Size: 1202 262
PspOMI G'GGCC_C
Cuts at: 0 502 1260 1464
Size: 502 758 204
Fragments arranged by size:
758 502 204
PstI C_TGCA'G
Cuts at: 0 388 1464
Size: 388 1076
PvuII CAG'CTG
Cuts at: 0 1166 1313 1464
Size: 1166 147 151
Fragments arranged by size:
1166 151 147
SacII CC_GC'GG
Cuts at: 0 459 1464
Size: 459 1005
SfcI C'TryA_G
Cuts at: 0 384 1464
Size: 384 1080
SfoI GGC'GCC
Cuts at: 0 7 1016 1464
Size: 7 1009 448
Fragments arranged by size:
1009 448 7
SmlI C'TyrA_G
Cuts at: 0 982 1129 1375 1464
Size: 982 147 246 89
Fragments arranged by size:
982 246 147 89
StuI AGG'CCT
Cuts at: 0 487 1464
Size: 487 977
StyI C'CwwG_G
Cuts at: 0 1358 1415 1464
Size: 1358 57 49
Fragments arranged by size:
1358 57 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1114 1210 1464
Size: 1114 96 254
Fragments arranged by size:
1114 254 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 718 750 1464
Size: 718 32 714
Fragments arranged by size:
718 714 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 1464
Size: 99 1365
XmnI GAAnn'nnTTC
Cuts at: 0 235 640 1204 1464
Size: 235 405 564 260
Fragments arranged by size:
564 405 260 235
Enzymes that do cut:
AarI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BglI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraIII EarI Eco57MI EcoO109I
EcoRI HaeII KasI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PspOMI PstI PvuII SacII SfcI
SfoI SmlI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmtI Bpu10I
BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI