(Linear) (Six-base) MAPSORT of: KIBB5839.seq  Check: 5458  from: 1  to: 609



KIBB5839.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     609 
   Size:        131     478
 
AfeI AGC'GCT

Cuts at:      0     373     609 
   Size:        373     236
 
AvrII C'CTAG_G

Cuts at:      0     219     609 
   Size:        219     390
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      49     133     577     609 
   Size:         49      84     444      32

  Fragments arranged by size: 

                 444      84      49      32
 
BclI T'GATC_A

Cuts at:      0     497     609 
   Size:        497     112
 
BlpI GC'TnA_GC

Cuts at:      0     469     609 
   Size:        469     140
 
Bme1580I G_kGCm'C

Cuts at:      0     171     609 
   Size:        171     438
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     200     609 
   Size:        200     409
 
BsaHI Gr'CG_yC

Cuts at:      0     461     609 
   Size:        461     148
 
BseYI C'CCAG_C

Cuts at:      0     210     609 
   Size:        210     399
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     609 
   Size:        474     135
 
Bsp1286I G_dGCh'C

Cuts at:      0     171     609 
   Size:        171     438
 
BsrDI GCAATG_nn'

Cuts at:      0     267     609 
   Size:        267     342
 
BstBI TT'CG_AA

Cuts at:      0     318     609 
   Size:        318     291
 
Bsu36I CC'TnA_GG

Cuts at:      0     335     609 
   Size:        335     274
 
BtgI C'CryG_G

Cuts at:      0     179     609 
   Size:        179     430
 
EaeI y'GGCC_r

Cuts at:      0     533     609 
   Size:        533      76
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     327     609 
   Size:        327     282
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     609 
   Size:        131     478
 
EcoO109I rG'GnC_Cy

Cuts at:      0     238     609 
   Size:        238     371
 
FspI TGC'GCA

Cuts at:      0     283     609 
   Size:        283     326
 
HaeII r_GCGC'y

Cuts at:      0     217     375     609 
   Size:        217     158     234

  Fragments arranged by size: 

                 234     217     158
 
MslI CAynn'nnrTG

Cuts at:      0     295     609 
   Size:        295     314
 
NcoI C'CATG_G

Cuts at:      0     179     609 
   Size:        179     430
 
PasI CC'CwG_GG

Cuts at:      0     171     609 
   Size:        171     438
 
PstI C_TGCA'G

Cuts at:      0      59     609 
   Size:         59     550
 
ScaI AGT'ACT

Cuts at:      0     406     609 
   Size:        406     203
 
SfcI C'TryA_G

Cuts at:      0      55     345     547     609 
   Size:         55     290     202      62

  Fragments arranged by size: 

                 290     202      62      55
 
StyI C'CwwG_G

Cuts at:      0     179     219     609 
   Size:        179      40     390

  Fragments arranged by size: 

                 390     179      40
 
TatI w'GTAC_w

Cuts at:      0     404     609 
   Size:        404     205
 
XbaI T'CTAG_A

Cuts at:      0     432     609 
   Size:        432     177
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     586     609 
   Size:        586      23
 

 Enzymes that do cut:

     AcuI     AfeI    AvrII     BbsI     BclI     BlpI Bme1580I     BsaI
    BsaHI    BseYI     BsgI Bsp1286I    BsrDI    BstBI   Bsu36I     BtgI
     EaeI     EciI  Eco57MI EcoO109I     FspI    HaeII     MslI     NcoI
     PasI     PstI     ScaI     SfcI     StyI     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XhoI     XmaI     XmnI     ZraI