Restriction map for
pF1KB5824
(Linear) (Six-base) MAPSORT of: KIBB5824.seq Check: 1338 from: 1 to: 1428
KIBB5824.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 137 1428
Size: 137 1291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 556 821 1387 1428
Size: 113 443 265 566 41
Fragments arranged by size:
566 443 265 113 41
AflIII A'CryG_T
Cuts at: 0 1042 1428
Size: 1042 386
ApoI r'AATT_y
Cuts at: 0 205 547 1284 1428
Size: 205 342 737 144
Fragments arranged by size:
737 342 205 144
AvaI C'yCGr_G
Cuts at: 0 827 1428
Size: 827 601
BamHI G'GATC_C
Cuts at: 0 1090 1428
Size: 1090 338
BanI G'GyrC_C
Cuts at: 0 52 1081 1428
Size: 52 1029 347
Fragments arranged by size:
1029 347 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 1428
Size: 26 1402
BciVI GTATCCnnnnn_n'
Cuts at: 0 978 1428
Size: 978 450
BclI T'GATC_A
Cuts at: 0 1050 1428
Size: 1050 378
BglII A'GATC_T
Cuts at: 0 164 1428
Size: 164 1264
Bme1580I G_kGCm'C
Cuts at: 0 11 1428
Size: 11 1417
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 626 854 1428
Size: 33 593 228 574
Fragments arranged by size:
593 574 228 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 509 629 887 927 1428
Size: 271 238 120 258 40 501
Fragments arranged by size:
501 271 258 238 120 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1428
Size: 790 638
BsaBI GATnn'nnATC
Cuts at: 0 169 1428
Size: 169 1259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 281 701 1428
Size: 281 420 727
Fragments arranged by size:
727 420 281
BseYI C'CCAG_C
Cuts at: 0 1034 1428
Size: 1034 394
Bsp1286I G_dGCh'C
Cuts at: 0 11 1428
Size: 11 1417
BspHI T'CATG_A
Cuts at: 0 189 1428
Size: 189 1239
BsrDI GCAATG_nn'
Cuts at: 0 605 1428
Size: 605 823
BstYI r'GATC_y
Cuts at: 0 164 1090 1428
Size: 164 926 338
Fragments arranged by size:
926 338 164
BtgI C'CryG_G
Cuts at: 0 619 1428
Size: 619 809
BtsI GCAGTG_nn'
Cuts at: 0 11 113 1428
Size: 11 102 1315
Fragments arranged by size:
1315 102 11
ClaI AT'CG_AT
Cuts at: 0 701 1428
Size: 701 727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 483 518 1428
Size: 483 35 910
Fragments arranged by size:
910 483 35
EarI CTCTTCn'nnn_
Cuts at: 0 532 537 1428
Size: 532 5 891
Fragments arranged by size:
891 532 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 271 509 556 629 821 887 927
Size: 113 158 238 47 73 192 66 40
Cuts at: 927 1387 1428
Size: 460 41
Fragments arranged by size:
460 238 192 158 113 73 66 47
41 40
EcoO109I rG'GnC_Cy
Cuts at: 0 824 1428
Size: 824 604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 1377 1428
Size: 88 1289 51
Fragments arranged by size:
1289 88 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 733 765 1428
Size: 559 32 142 32 663
Fragments arranged by size:
663 559 142 32 32
HincII GTy'rAC
Cuts at: 0 1317 1428
Size: 1317 111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1428
Size: 392 1036
MslI CAynn'nnrTG
Cuts at: 0 194 301 1428
Size: 194 107 1127
Fragments arranged by size:
1127 194 107
MspA1I CmG'CkG
Cuts at: 0 641 1113 1428
Size: 641 472 315
Fragments arranged by size:
641 472 315
NcoI C'CATG_G
Cuts at: 0 619 1428
Size: 619 809
NdeI CA'TA_TG
Cuts at: 0 516 1055 1428
Size: 516 539 373
Fragments arranged by size:
539 516 373
NspI r_CATG'y
Cuts at: 0 306 1046 1428
Size: 306 740 382
Fragments arranged by size:
740 382 306
PasI CC'CwG_GG
Cuts at: 0 42 1428
Size: 42 1386
PciI A'CATG_T
Cuts at: 0 1042 1428
Size: 1042 386
PshAI GACnn'nnGTC
Cuts at: 0 641 1428
Size: 641 787
PvuII CAG'CTG
Cuts at: 0 641 1428
Size: 641 787
ScaI AGT'ACT
Cuts at: 0 256 590 940 1428
Size: 256 334 350 488
Fragments arranged by size:
488 350 334 256
SfcI C'TryA_G
Cuts at: 0 258 1428
Size: 258 1170
SmlI C'TyrA_G
Cuts at: 0 805 1428
Size: 805 623
SphI G_CATG'C
Cuts at: 0 306 1428
Size: 306 1122
SspI AAT'ATT
Cuts at: 0 153 241 1310 1428
Size: 153 88 1069 118
Fragments arranged by size:
1069 153 118 88
StyI C'CwwG_G
Cuts at: 0 619 688 1428
Size: 619 69 740
Fragments arranged by size:
740 619 69
TatI w'GTAC_w
Cuts at: 0 254 588 938 1428
Size: 254 334 350 490
Fragments arranged by size:
490 350 334 254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 1428
Size: 868 32 528
Fragments arranged by size:
868 528 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 569 1428
Size: 569 859
XmnI GAAnn'nnTTC
Cuts at: 0 404 1428
Size: 404 1024
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI BamHI BanI BbsI
BciVI BclI BglII Bme1580I BmrI BpmI BpuEI BsaBI
BseRI BseYI Bsp1286I BspHI BsrDI BstYI BtgI BtsI
ClaI CspCI EarI Eco57MI EcoO109I FalI Hin4I HincII
MmeI MslI MspA1I NcoI NdeI NspI PasI PciI
PshAI PvuII ScaI SfcI SmlI SphI SspI StyI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BfrBI
BglI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI