Restriction map for
pF1KB5818
(Linear) (Six-base) MAPSORT of: KIBB5818.seq Check: 4581 from: 1 to: 1428
KIBB5818.orf
With 182 enzymes: SgfI *
September 25, 2012 15:34 ..
AccI GT'mk_AC
Cuts at: 0 371 1428
Size: 371 1057
AflIII A'CryG_T
Cuts at: 0 1106 1428
Size: 1106 322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 194 226 953 985 1428
Size: 194 32 727 32 443
Fragments arranged by size:
727 443 194 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 277 1428
Size: 277 1151
ApaI G_GGCC'C
Cuts at: 0 1282 1408 1428
Size: 1282 126 20
Fragments arranged by size:
1282 126 20
AvaI C'yCGr_G
Cuts at: 0 102 443 1428
Size: 102 341 985
Fragments arranged by size:
985 341 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1160 1193 1428
Size: 1160 33 235
Fragments arranged by size:
1160 235 33
BanI G'GyrC_C
Cuts at: 0 616 723 1428
Size: 616 107 705
Fragments arranged by size:
705 616 107
BanII G_rGCy'C
Cuts at: 0 323 1282 1408 1428
Size: 323 959 126 20
Fragments arranged by size:
959 323 126 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 949 1226 1428
Size: 949 277 202
Fragments arranged by size:
949 277 202
BglI GCCn_nnn'nGGC
Cuts at: 0 261 717 1428
Size: 261 456 711
Fragments arranged by size:
711 456 261
BlpI GC'TnA_GC
Cuts at: 0 1309 1428
Size: 1309 119
Bme1580I G_kGCm'C
Cuts at: 0 1282 1408 1428
Size: 1282 126 20
Fragments arranged by size:
1282 126 20
BmgBI CAC'GTC
Cuts at: 0 77 1428
Size: 77 1351
BmrI ACTGGGnnnn_n'
Cuts at: 0 287 1428
Size: 287 1141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1249 1428
Size: 749 500 179
Fragments arranged by size:
749 500 179
Bpu10I CC'TnA_GC
Cuts at: 0 1159 1428
Size: 1159 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1428
Size: 698 730
BsaI GGTCTCn'nnnn_
Cuts at: 0 1225 1428
Size: 1225 203
BsaWI w'CCGG_w
Cuts at: 0 126 1428
Size: 126 1302
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 722 725 752 755 1428
Size: 722 3 27 3 673
Fragments arranged by size:
722 673 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 478 697 746 1428
Size: 145 333 219 49 682
Fragments arranged by size:
682 333 219 145 49
BseYI C'CCAG_C
Cuts at: 0 415 612 878 1275 1428
Size: 415 197 266 397 153
Fragments arranged by size:
415 397 266 197 153
BsiEI CG_ry'CG
Cuts at: 0 552 1428
Size: 552 876
BsiHKAI G_wGCw'C
Cuts at: 0 323 1326 1428
Size: 323 1003 102
Fragments arranged by size:
1003 323 102
BsmI GAATG_Cn'
Cuts at: 0 366 1428
Size: 366 1062
BsmBI CGTCTCn'nnnn_
Cuts at: 0 101 1428
Size: 101 1327
Bsp1286I G_dGCh'C
Cuts at: 0 323 1282 1326 1408 1428
Size: 323 959 44 82 20
Fragments arranged by size:
959 323 82 44 20
BspEI T'CCGG_A
Cuts at: 0 126 1428
Size: 126 1302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 872 1091 1428
Size: 872 219 337
Fragments arranged by size:
872 337 219
BsrDI GCAATG_nn'
Cuts at: 0 323 1428
Size: 323 1105
BsrGI T'GTAC_A
Cuts at: 0 1109 1428
Size: 1109 319
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1071 1428
Size: 1071 357
BstEII G'GTnAC_C
Cuts at: 0 1255 1428
Size: 1255 173
BstYI r'GATC_y
Cuts at: 0 906 1428
Size: 906 522
Bsu36I CC'TnA_GG
Cuts at: 0 1213 1428
Size: 1213 215
BtgI C'CryG_G
Cuts at: 0 152 488 812 1428
Size: 152 336 324 616
Fragments arranged by size:
616 336 324 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 217 1428
Size: 217 1211
BtsI GCAGTG_nn'
Cuts at: 0 44 1428
Size: 44 1384
EaeI y'GGCC_r
Cuts at: 0 95 228 506 549 1428
Size: 95 133 278 43 879
Fragments arranged by size:
879 278 133 95 43
EagI C'GGCC_G
Cuts at: 0 549 1428
Size: 549 879
EarI CTCTTCn'nnn_
Cuts at: 0 344 1428
Size: 344 1084
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 304 1428
Size: 304 1124
EcoICRI GAG'CTC
Cuts at: 0 321 1428
Size: 321 1107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1249 1428
Size: 749 500 179
Fragments arranged by size:
749 500 179
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 761 1428
Size: 628 133 667
Fragments arranged by size:
667 628 133
EcoO109I rG'GnC_Cy
Cuts at: 0 192 281 1080 1405 1428
Size: 192 89 799 325 23
Fragments arranged by size:
799 325 192 89 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 159 918 1428
Size: 159 759 510
Fragments arranged by size:
759 510 159
HincII GTy'rAC
Cuts at: 0 372 585 1428
Size: 372 213 843
Fragments arranged by size:
843 372 213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1428
Size: 1162 266
MscI TGG'CCA
Cuts at: 0 230 508 1428
Size: 230 278 920
Fragments arranged by size:
920 278 230
MslI CAynn'nnrTG
Cuts at: 0 1138 1382 1428
Size: 1138 244 46
Fragments arranged by size:
1138 244 46
MspA1I CmG'CkG
Cuts at: 0 30 419 1428
Size: 30 389 1009
Fragments arranged by size:
1009 389 30
NcoI C'CATG_G
Cuts at: 0 812 1428
Size: 812 616
NspI r_CATG'y
Cuts at: 0 669 1110 1227 1428
Size: 669 441 117 201
Fragments arranged by size:
669 441 201 117
PasI CC'CwG_GG
Cuts at: 0 259 1428
Size: 259 1169
PciI A'CATG_T
Cuts at: 0 1106 1428
Size: 1106 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 1288 1401 1428
Size: 1288 113 27
Fragments arranged by size:
1288 113 27
PfoI T'CCnGG_A
Cuts at: 0 1202 1428
Size: 1202 226
PpuMI rG'GwC_Cy
Cuts at: 0 1080 1428
Size: 1080 348
PspOMI G'GGCC_C
Cuts at: 0 1278 1404 1428
Size: 1278 126 24
Fragments arranged by size:
1278 126 24
PstI C_TGCA'G
Cuts at: 0 173 1032 1428
Size: 173 859 396
Fragments arranged by size:
859 396 173
SacI G_AGCT'C
Cuts at: 0 323 1428
Size: 323 1105
SalI G'TCGA_C
Cuts at: 0 370 1428
Size: 370 1058
ScaI AGT'ACT
Cuts at: 0 859 1428
Size: 859 569
SfcI C'TryA_G
Cuts at: 0 169 1028 1428
Size: 169 859 400
Fragments arranged by size:
859 400 169
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 261 1428
Size: 261 1167
SmaI CCC'GGG
Cuts at: 0 445 1428
Size: 445 983
SmlI C'TyrA_G
Cuts at: 0 713 1428
Size: 713 715
SphI G_CATG'C
Cuts at: 0 669 1428
Size: 669 759
SspI AAT'ATT
Cuts at: 0 1126 1428
Size: 1126 302
StuI AGG'CCT
Cuts at: 0 496 640 767 788 1211 1428
Size: 496 144 127 21 423 217
Fragments arranged by size:
496 423 217 144 127 21
StyI C'CwwG_G
Cuts at: 0 197 284 599 812 902 1244 1428
Size: 197 87 315 213 90 342 184
Fragments arranged by size:
342 315 213 197 184 90 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 643 1028 1428
Size: 604 39 385 400
Fragments arranged by size:
604 400 385 39
TatI w'GTAC_w
Cuts at: 0 857 1109 1397 1428
Size: 857 252 288 31
Fragments arranged by size:
857 288 252 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 722 725 754 757 1428
Size: 722 3 29 3 671
Fragments arranged by size:
722 671 29 3 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 819 1428
Size: 342 477 609
Fragments arranged by size:
609 477 342
XmaI C'CCGG_G
Cuts at: 0 443 1428
Size: 443 985
XmnI GAAnn'nnTTC
Cuts at: 0 139 1428
Size: 139 1289
Enzymes that do cut:
AccI AflIII AloI AlwNI ApaI AvaI BaeI BanI
BanII BbsI BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI
BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HincII MmeI MscI MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PpuMI PspOMI PstI SacI SalI
ScaI SfcI SfiI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BplI BsaAI BsaBI BsaHI BsgI BsiWI
BspHI BsrBI BsrFI BssHII BssSI BstBI BstXI BstZ17I
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SanDI SapI
SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI
SwaI Tth111I XbaI XhoI ZraI