Restriction map for
pF1KB5816
(Linear) (Six-base) MAPSORT of: KIBB5816.seq Check: 3793 from: 1 to: 2625
KIBB5816.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AatII G_ACGT'C
Cuts at: 0 99 359 2625
Size: 99 260 2266
Fragments arranged by size:
2266 260 99
Acc65I G'GTAC_C
Cuts at: 0 1325 2625
Size: 1325 1300
AfeI AGC'GCT
Cuts at: 0 193 511 1163 2625
Size: 193 318 652 1462
Fragments arranged by size:
1462 652 318 193
AhdI GACnn_n'nnGTC
Cuts at: 0 664 2625
Size: 664 1961
AleI CACnn'nnGTG
Cuts at: 0 1070 2625
Size: 1070 1555
AlwNI CAG_nnn'CTG
Cuts at: 0 1108 2118 2379 2625
Size: 1108 1010 261 246
Fragments arranged by size:
1108 1010 261 246
ApaI G_GGCC'C
Cuts at: 0 319 648 1940 2625
Size: 319 329 1292 685
Fragments arranged by size:
1292 685 329 319
ApoI r'AATT_y
Cuts at: 0 2158 2625
Size: 2158 467
AvaI C'yCGr_G
Cuts at: 0 638 1295 2625
Size: 638 657 1330
Fragments arranged by size:
1330 657 638
AvrII C'CTAG_G
Cuts at: 0 882 2625
Size: 882 1743
BanI G'GyrC_C
Cuts at: 0 1325 2625
Size: 1325 1300
BanII G_rGCy'C
Cuts at: 0 319 648 848 1361 1477 1940 2625
Size: 319 329 200 513 116 463 685
Fragments arranged by size:
685 513 463 329 319 200 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 454 660 935 1022 1993 2625
Size: 454 206 275 87 971 632
Fragments arranged by size:
971 632 454 275 206 87
BbvCI CC'TCA_GC
Cuts at: 0 335 1136 2625
Size: 335 801 1489
Fragments arranged by size:
1489 801 335
BglI GCCn_nnn'nGGC
Cuts at: 0 643 2625
Size: 643 1982
BglII A'GATC_T
Cuts at: 0 1427 2625
Size: 1427 1198
BlpI GC'TnA_GC
Cuts at: 0 1471 2267 2625
Size: 1471 796 358
Fragments arranged by size:
1471 796 358
Bme1580I G_kGCm'C
Cuts at: 0 319 648 1940 2625
Size: 319 329 1292 685
Fragments arranged by size:
1292 685 329 319
BmrI ACTGGGnnnn_n'
Cuts at: 0 258 2128 2625
Size: 258 1870 497
Fragments arranged by size:
1870 497 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 279 1326 2052 2625
Size: 213 66 1047 726 573
Fragments arranged by size:
1047 726 573 213 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 893 1246 1996 2625
Size: 823 70 353 750 629
Fragments arranged by size:
823 750 629 353 70
Bpu10I CC'TnA_GC
Cuts at: 0 335 1136 2625
Size: 335 801 1489
Fragments arranged by size:
1489 801 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 2625
Size: 1150 1475
BsaI GGTCTCn'nnnn_
Cuts at: 0 2558 2625
Size: 2558 67
BsaHI Gr'CG_yC
Cuts at: 0 96 356 1214 1627 2625
Size: 96 260 858 413 998
Fragments arranged by size:
998 858 413 260 96
BsaWI w'CCGG_w
Cuts at: 0 176 536 1058 2625
Size: 176 360 522 1567
Fragments arranged by size:
1567 522 360 176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 255 457 595 826 829 832 835 1153
Size: 255 202 138 231 3 3 3 318
Cuts at: 1153 1156 1165 1386 1726 1729 1732 1736 1768
Size: 3 9 221 340 3 3 4 32
Cuts at: 1768 1822 2011 2625
Size: 54 189 614
Fragments arranged by size:
614 340 318 255 231 221 202 189
138 54 32 9 4 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 301 399 847 1361 1391 1529 1725 2274
Size: 301 98 448 514 30 138 196 549
Cuts at: 2274 2300 2625
Size: 26 325
Fragments arranged by size:
549 514 448 325 301 196 138 98
30 26
BsiEI CG_ry'CG
Cuts at: 0 27 72 1243 1407 2625
Size: 27 45 1171 164 1218
Fragments arranged by size:
1218 1171 164 45 27
BsiHKAI G_wGCw'C
Cuts at: 0 260 2625
Size: 260 2365
BsmI GAATG_Cn'
Cuts at: 0 1854 2625
Size: 1854 771
BsmBI CGTCTCn'nnnn_
Cuts at: 0 487 1459 2625
Size: 487 972 1166
Fragments arranged by size:
1166 972 487
Bsp1286I G_dGCh'C
Cuts at: 0 260 319 648 848 1361 1477 1940 2625
Size: 260 59 329 200 513 116 463 685
Fragments arranged by size:
685 513 463 329 260 200 116 59
BspEI T'CCGG_A
Cuts at: 0 536 2625
Size: 536 2089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1264 1448 1850 2625
Size: 1264 184 402 775
Fragments arranged by size:
1264 775 402 184
BsrBI CCG'CTC
Cuts at: 0 1127 1797 2625
Size: 1127 670 828
Fragments arranged by size:
1127 828 670
BsrDI GCAATG_nn'
Cuts at: 0 407 2625
Size: 407 2218
BssSI C'ACGA_G
Cuts at: 0 1641 2625
Size: 1641 984
BstAPI GCAn_nnn'nTGC
Cuts at: 0 549 1545 2625
Size: 549 996 1080
Fragments arranged by size:
1080 996 549
BstEII G'GTnAC_C
Cuts at: 0 1395 2625
Size: 1395 1230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2308 2625
Size: 2308 317
BstYI r'GATC_y
Cuts at: 0 1427 2625
Size: 1427 1198
Bsu36I CC'TnA_GG
Cuts at: 0 1751 1763 2625
Size: 1751 12 862
Fragments arranged by size:
1751 862 12
BtgI C'CryG_G
Cuts at: 0 132 1217 1237 2625
Size: 132 1085 20 1388
Fragments arranged by size:
1388 1085 132 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1263 2625
Size: 1263 1362
BtsI GCAGTG_nn'
Cuts at: 0 1416 2625
Size: 1416 1209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1896 1931 2625
Size: 1896 35 694
Fragments arranged by size:
1896 694 35
EaeI y'GGCC_r
Cuts at: 0 135 1128 1240 1568 2625
Size: 135 993 112 328 1057
Fragments arranged by size:
1057 993 328 135 112
EagI C'GGCC_G
Cuts at: 0 1240 2625
Size: 1240 1385
EarI CTCTTCn'nnn_
Cuts at: 0 45 998 1270 2039 2625
Size: 45 953 272 769 586
Fragments arranged by size:
953 769 586 272 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2425 2625
Size: 2425 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 893 1246 1996 2625
Size: 823 70 353 750 629
Fragments arranged by size:
823 750 629 353 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 805 1693 1766 2625
Size: 805 888 73 859
Fragments arranged by size:
888 859 805 73
EcoO109I rG'GnC_Cy
Cuts at: 0 315 644 645 1491 1936 2167 2376 2458
Size: 315 329 1 846 445 231 209 82
Cuts at: 2458 2592 2625
Size: 134 33
Fragments arranged by size:
846 445 329 315 231 209 134 82
33 1
FspI TGC'GCA
Cuts at: 0 1273 1549 1846 2182 2625
Size: 1273 276 297 336 443
Fragments arranged by size:
1273 443 336 297 276
HaeII r_GCGC'y
Cuts at: 0 147 195 513 1165 1351 1709 1745 2274
Size: 147 48 318 652 186 358 36 529
Cuts at: 2274 2625
Size: 351
Fragments arranged by size:
652 529 358 351 318 186 147 48
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 650 682 1096 1102 1128 1134 1893 1925
Size: 650 32 414 6 26 6 759 32
Cuts at: 1925 2625
Size: 700
Fragments arranged by size:
759 700 650 414 32 32 26 6
6
HincII GTy'rAC
Cuts at: 0 1416 2625
Size: 1416 1209
HpaI GTT'AAC
Cuts at: 0 1416 2625
Size: 1416 1209
KpnI G_GTAC'C
Cuts at: 0 1329 2625
Size: 1329 1296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1094 1414 2129 2625
Size: 962 132 320 715 496
Fragments arranged by size:
962 715 496 320 132
MslI CAynn'nnrTG
Cuts at: 0 575 608 1070 2625
Size: 575 33 462 1555
Fragments arranged by size:
1555 575 462 33
MspA1I CmG'CkG
Cuts at: 0 1219 1239 1245 1736 2625
Size: 1219 20 6 491 889
Fragments arranged by size:
1219 889 491 20 6
NotI GC'GGCC_GC
Cuts at: 0 1240 2625
Size: 1240 1385
NspI r_CATG'y
Cuts at: 0 2206 2625
Size: 2206 419
PasI CC'CwG_GG
Cuts at: 0 341 2373 2625
Size: 341 2032 252
Fragments arranged by size:
2032 341 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 325 1587 2058 2625
Size: 325 1262 471 567
Fragments arranged by size:
1262 567 471 325
PfoI T'CCnGG_A
Cuts at: 0 710 800 1457 2625
Size: 710 90 657 1168
Fragments arranged by size:
1168 710 657 90
PpuMI rG'GwC_Cy
Cuts at: 0 1491 2167 2592 2625
Size: 1491 676 425 33
Fragments arranged by size:
1491 676 425 33
PspOMI G'GGCC_C
Cuts at: 0 315 644 1936 2625
Size: 315 329 1292 689
Fragments arranged by size:
1292 689 329 315
PstI C_TGCA'G
Cuts at: 0 1445 2445 2625
Size: 1445 1000 180
Fragments arranged by size:
1445 1000 180
PvuI CG_AT'CG
Cuts at: 0 27 2625
Size: 27 2598
SacII CC_GC'GG
Cuts at: 0 1220 1240 2625
Size: 1220 20 1385
Fragments arranged by size:
1385 1220 20
SfcI C'TryA_G
Cuts at: 0 1441 2441 2625
Size: 1441 1000 184
Fragments arranged by size:
1441 1000 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 643 2625
Size: 643 1982
SmaI CCC'GGG
Cuts at: 0 640 1297 2625
Size: 640 657 1328
Fragments arranged by size:
1328 657 640
SmlI C'TyrA_G
Cuts at: 0 1165 2625
Size: 1165 1460
SphI G_CATG'C
Cuts at: 0 2206 2625
Size: 2206 419
StuI AGG'CCT
Cuts at: 0 881 2625
Size: 881 1744
StyI C'CwwG_G
Cuts at: 0 882 1184 1208 1859 2569 2625
Size: 882 302 24 651 710 56
Fragments arranged by size:
882 710 651 302 56 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 581 620 1383 1543 1962 2625
Size: 415 166 39 763 160 419 663
Fragments arranged by size:
763 663 419 415 166 160 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 2544 2576 2625
Size: 1053 32 1459 32 49
Fragments arranged by size:
1459 1053 49 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2479 2625
Size: 2479 146
XmaI C'CCGG_G
Cuts at: 0 638 1295 2625
Size: 638 657 1330
Fragments arranged by size:
1330 657 638
XmnI GAAnn'nnTTC
Cuts at: 0 451 2625
Size: 451 2174
ZraI GAC'GTC
Cuts at: 0 97 357 2625
Size: 97 260 2268
Fragments arranged by size:
2268 260 97
Enzymes that do cut:
AatII Acc65I AfeI AhdI AleI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII HpaI KpnI MmeI MslI MspA1I NotI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuI
SacII SfcI SfiI SmaI SmlI SphI StuI StyI
TaqII TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AflII AflIII AgeI AlfI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsaBI
BsaXI BsgI BsiWI BspHI BsrFI BsrGI BssHII BstBI
BstZ17I ClaI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FalI FseI FspAI HindIII KasI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XhoI