Restriction map for
pF1KB5808
(Linear) (Six-base) MAPSORT of: KIBB5808.seq Check: 8148 from: 1 to: 1362
KIBB5808.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 257 628 1362
Size: 257 371 734
Fragments arranged by size:
734 371 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 1362
Size: 1219 143
AflIII A'CryG_T
Cuts at: 0 371 373 1121 1362
Size: 371 2 748 241
Fragments arranged by size:
748 371 241 2
AlwNI CAG_nnn'CTG
Cuts at: 0 954 1362
Size: 954 408
ApaI G_GGCC'C
Cuts at: 0 917 1362
Size: 917 445
AvaI C'yCGr_G
Cuts at: 0 251 1362
Size: 251 1111
BanI G'GyrC_C
Cuts at: 0 34 219 1097 1362
Size: 34 185 878 265
Fragments arranged by size:
878 265 185 34
BanII G_rGCy'C
Cuts at: 0 130 550 566 761 917 1040 1362
Size: 130 420 16 195 156 123 322
Fragments arranged by size:
420 322 195 156 130 123 16
BbeI G_GCGC'C
Cuts at: 0 1101 1362
Size: 1101 261
BglI GCCn_nnn'nGGC
Cuts at: 0 620 1362
Size: 620 742
BlpI GC'TnA_GC
Cuts at: 0 124 544 760 991 1362
Size: 124 420 216 231 371
Fragments arranged by size:
420 371 231 216 124
Bme1580I G_kGCm'C
Cuts at: 0 39 96 224 917 1362
Size: 39 57 128 693 445
Fragments arranged by size:
693 445 128 57 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 1290 1362
Size: 192 1098 72
Fragments arranged by size:
1098 192 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 982 1051 1267 1362
Size: 624 358 69 216 95
Fragments arranged by size:
624 358 216 95 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 110 1010 1362
Size: 110 900 352
Fragments arranged by size:
900 352 110
BsaAI yAC'GTr
Cuts at: 0 374 1362
Size: 374 988
BsaHI Gr'CG_yC
Cuts at: 0 1098 1362
Size: 1098 264
BsaWI w'CCGG_w
Cuts at: 0 488 884 1362
Size: 488 396 478
Fragments arranged by size:
488 478 396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 239 1048 1266 1362
Size: 226 13 809 218 96
Fragments arranged by size:
809 226 218 96 13
BseYI C'CCAG_C
Cuts at: 0 223 618 943 1108 1356 1362
Size: 223 395 325 165 248 6
Fragments arranged by size:
395 325 248 223 165 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 679 810 1362
Size: 665 14 131 552
Fragments arranged by size:
665 552 131 14
BsiHKAI G_wGCw'C
Cuts at: 0 202 566 1362
Size: 202 364 796
Fragments arranged by size:
796 364 202
Bsp1286I G_dGCh'C
Cuts at: 0 39 96 130 202 224 550 566 761
Size: 39 57 34 72 22 326 16 195
Cuts at: 761 917 1040 1362
Size: 156 123 322
Fragments arranged by size:
326 322 195 156 123 72 57 39
34 22 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 58 1362
Size: 58 1304
BsrDI GCAATG_nn'
Cuts at: 0 285 764 1362
Size: 285 479 598
Fragments arranged by size:
598 479 285
BsrFI r'CCGG_y
Cuts at: 0 434 904 1100 1362
Size: 434 470 196 262
Fragments arranged by size:
470 434 262 196
BsrGI T'GTAC_A
Cuts at: 0 368 1362
Size: 368 994
BstAPI GCAn_nnn'nTGC
Cuts at: 0 954 1362
Size: 954 408
BstBI TT'CG_AA
Cuts at: 0 452 1362
Size: 452 910
BstEII G'GTnAC_C
Cuts at: 0 408 524 1362
Size: 408 116 838
Fragments arranged by size:
838 408 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 1362
Size: 171 1191
BstYI r'GATC_y
Cuts at: 0 49 666 1362
Size: 49 617 696
Fragments arranged by size:
696 617 49
BstZ17I GTA'TAC
Cuts at: 0 629 1362
Size: 629 733
Bsu36I CC'TnA_GG
Cuts at: 0 1081 1362
Size: 1081 281
BtsI GCAGTG_nn'
Cuts at: 0 1066 1362
Size: 1066 296
DraIII CAC_nnn'GTG
Cuts at: 0 657 1362
Size: 657 705
EaeI y'GGCC_r
Cuts at: 0 2 1362
Size: 2 1360
EarI CTCTTCn'nnn_
Cuts at: 0 554 1362
Size: 554 808
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 98 233 1362
Size: 98 135 1129
Fragments arranged by size:
1129 135 98
EcoICRI GAG'CTC
Cuts at: 0 564 1362
Size: 564 798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 982 1051 1219 1267 1362
Size: 624 358 69 168 48 95
Fragments arranged by size:
624 358 168 95 69 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 1362
Size: 163 1199
EcoO109I rG'GnC_Cy
Cuts at: 0 43 141 914 1344 1362
Size: 43 98 773 430 18
Fragments arranged by size:
773 430 98 43 18
EcoRV GAT'ATC
Cuts at: 0 807 1362
Size: 807 555
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 354 1362
Size: 285 69 1008
Fragments arranged by size:
1008 285 69
HaeII r_GCGC'y
Cuts at: 0 998 1101 1362
Size: 998 103 261
Fragments arranged by size:
998 261 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 274 306 402 434 1261 1293
Size: 162 32 80 32 96 32 827 32
Cuts at: 1293 1362
Size: 69
Fragments arranged by size:
827 162 96 80 69 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1097 1362
Size: 1097 265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1362
Size: 890 472
MscI TGG'CCA
Cuts at: 0 4 1362
Size: 4 1358
MslI CAynn'nnrTG
Cuts at: 0 1298 1362
Size: 1298 64
MspA1I CmG'CkG
Cuts at: 0 618 663 1362
Size: 618 45 699
Fragments arranged by size:
699 618 45
NaeI GCC'GGC
Cuts at: 0 436 906 1102 1362
Size: 436 470 196 260
Fragments arranged by size:
470 436 260 196
NarI GG'CG_CC
Cuts at: 0 1098 1362
Size: 1098 264
NgoMIV G'CCGG_C
Cuts at: 0 434 904 1100 1362
Size: 434 470 196 262
Fragments arranged by size:
470 434 262 196
NspI r_CATG'y
Cuts at: 0 465 1125 1362
Size: 465 660 237
Fragments arranged by size:
660 465 237
PasI CC'CwG_GG
Cuts at: 0 39 61 88 1362
Size: 39 22 27 1274
Fragments arranged by size:
1274 39 27 22
PciI A'CATG_T
Cuts at: 0 1121 1362
Size: 1121 241
PmlI CAC'GTG
Cuts at: 0 374 1362
Size: 374 988
PspOMI G'GGCC_C
Cuts at: 0 913 1362
Size: 913 449
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 352 384 1362
Size: 352 32 978
Fragments arranged by size:
978 352 32
PstI C_TGCA'G
Cuts at: 0 1061 1362
Size: 1061 301
PvuII CAG'CTG
Cuts at: 0 618 663 1362
Size: 618 45 699
Fragments arranged by size:
699 618 45
SacI G_AGCT'C
Cuts at: 0 566 1362
Size: 566 796
SapI GCTCTTCn'nnn_
Cuts at: 0 554 1362
Size: 554 808
ScaI AGT'ACT
Cuts at: 0 430 1362
Size: 430 932
SfcI C'TryA_G
Cuts at: 0 439 1057 1362
Size: 439 618 305
Fragments arranged by size:
618 439 305
SfoI GGC'GCC
Cuts at: 0 1099 1362
Size: 1099 263
SgrAI Cr'CCGG_yG
Cuts at: 0 1100 1362
Size: 1100 262
StyI C'CwwG_G
Cuts at: 0 164 467 572 1129 1362
Size: 164 303 105 557 233
Fragments arranged by size:
557 303 233 164 105
TatI w'GTAC_w
Cuts at: 0 368 428 1362
Size: 368 60 934
Fragments arranged by size:
934 368 60
Tth111I GACn'n_nGTC
Cuts at: 0 928 1362
Size: 928 434
XmnI GAAnn'nnTTC
Cuts at: 0 448 1362
Size: 448 914
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BplI BpmI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PmlI PspOMI PsrI PstI PvuII SacI
SapI ScaI SfcI SfoI SgrAI StyI TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssHII BssSI BtgI BtgZI ClaI CspCI DraI DrdI
EagI EcoRI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XbaI XcmI
XhoI XmaI ZraI