Restriction map for
pF1KB5797
(Linear) (Six-base) MAPSORT of: KIBB5797.seq Check: 6231 from: 1 to: 2502
KIBB5797.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2286 2502
Size: 2286 216
AatII G_ACGT'C
Cuts at: 0 527 2502
Size: 527 1975
AccI GT'mk_AC
Cuts at: 0 494 563 2502
Size: 494 69 1939
Fragments arranged by size:
1939 494 69
AclI AA'CG_TT
Cuts at: 0 2497 2502
Size: 2497 5
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1396 1653 1717 2172 2502
Size: 1222 174 257 64 455 330
Fragments arranged by size:
1222 455 330 257 174 64
AfeI AGC'GCT
Cuts at: 0 2476 2502
Size: 2476 26
AflIII A'CryG_T
Cuts at: 0 1136 2502
Size: 1136 1366
AgeI A'CCGG_T
Cuts at: 0 864 1586 2502
Size: 864 722 916
Fragments arranged by size:
916 864 722
AleI CACnn'nnGTG
Cuts at: 0 1351 2502
Size: 1351 1151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 224 2318 2502
Size: 224 2094 184
Fragments arranged by size:
2094 224 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1424 1456 2502
Size: 1424 32 1046
Fragments arranged by size:
1424 1046 32
AlwNI CAG_nnn'CTG
Cuts at: 0 856 888 1494 1764 2121 2502
Size: 856 32 606 270 357 381
Fragments arranged by size:
856 606 381 357 270 32
ApaI G_GGCC'C
Cuts at: 0 443 2502
Size: 443 2059
ApaLI G'TGCA_C
Cuts at: 0 2255 2431 2502
Size: 2255 176 71
Fragments arranged by size:
2255 176 71
ApoI r'AATT_y
Cuts at: 0 347 2502
Size: 347 2155
AvaI C'yCGr_G
Cuts at: 0 302 959 1073 2502
Size: 302 657 114 1429
Fragments arranged by size:
1429 657 302 114
BamHI G'GATC_C
Cuts at: 0 1212 2502
Size: 1212 1290
BanI G'GyrC_C
Cuts at: 0 373 399 878 1611 2502
Size: 373 26 479 733 891
Fragments arranged by size:
891 733 479 373 26
BanII G_rGCy'C
Cuts at: 0 443 934 2502
Size: 443 491 1568
Fragments arranged by size:
1568 491 443
BbsI GAAGACnn'nnnn_
Cuts at: 0 1139 1484 2502
Size: 1139 345 1018
Fragments arranged by size:
1139 1018 345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 245 2502
Size: 211 34 2257
Fragments arranged by size:
2257 211 34
BlpI GC'TnA_GC
Cuts at: 0 1996 2202 2502
Size: 1996 206 300
Fragments arranged by size:
1996 300 206
Bme1580I G_kGCm'C
Cuts at: 0 443 1616 2259 2435 2502
Size: 443 1173 643 176 67
Fragments arranged by size:
1173 643 443 176 67
BmgBI CAC'GTC
Cuts at: 0 1145 2502
Size: 1145 1357
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 2502
Size: 228 2274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1095 1231 1257 2319 2502
Size: 198 897 136 26 1062 183
Fragments arranged by size:
1062 897 198 183 136 26
Bpu10I CC'TnA_GC
Cuts at: 0 706 1190 1819 2105 2502
Size: 706 484 629 286 397
Fragments arranged by size:
706 629 484 397 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 614 997 1279 2344 2502
Size: 539 75 383 282 1065 158
Fragments arranged by size:
1065 539 383 282 158 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 2140 2502
Size: 2140 362
BsaAI yAC'GTr
Cuts at: 0 447 492 2502
Size: 447 45 2010
Fragments arranged by size:
2010 447 45
BsaHI Gr'CG_yC
Cuts at: 0 524 1622 2502
Size: 524 1098 880
Fragments arranged by size:
1098 880 524
BsaWI w'CCGG_w
Cuts at: 0 768 864 1586 2502
Size: 768 96 722 916
Fragments arranged by size:
916 768 722 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1424 1454 1732 1762 2502
Size: 1424 30 278 30 740
Fragments arranged by size:
1424 740 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1146 1881 1927 2502
Size: 1146 735 46 575
Fragments arranged by size:
1146 735 575 46
BseYI C'CCAG_C
Cuts at: 0 1185 2207 2404 2462 2502
Size: 1185 1022 197 58 40
Fragments arranged by size:
1185 1022 197 58 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 783 1545 2502
Size: 433 350 762 957
Fragments arranged by size:
957 762 433 350
BsiEI CG_ry'CG
Cuts at: 0 150 2502
Size: 150 2352
BsiHKAI G_wGCw'C
Cuts at: 0 139 2106 2259 2435 2502
Size: 139 1967 153 176 67
Fragments arranged by size:
1967 176 153 139 67
Bsp1286I G_dGCh'C
Cuts at: 0 139 443 934 1616 2106 2259 2435 2502
Size: 139 304 491 682 490 153 176 67
Fragments arranged by size:
682 491 490 304 176 153 139 67
BspHI T'CATG_A
Cuts at: 0 1718 1958 2327 2502
Size: 1718 240 369 175
Fragments arranged by size:
1718 369 240 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 805 2286 2502
Size: 805 1481 216
Fragments arranged by size:
1481 805 216
BsrFI r'CCGG_y
Cuts at: 0 864 1586 2502
Size: 864 722 916
Fragments arranged by size:
916 864 722
BssSI C'ACGA_G
Cuts at: 0 220 2502
Size: 220 2282
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1417 2502
Size: 1417 1085
BstEII G'GTnAC_C
Cuts at: 0 1774 2502
Size: 1774 728
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1281 2373 2502
Size: 1281 1092 129
Fragments arranged by size:
1281 1092 129
BstYI r'GATC_y
Cuts at: 0 1212 1490 2502
Size: 1212 278 1012
Fragments arranged by size:
1212 1012 278
BstZ17I GTA'TAC
Cuts at: 0 495 564 2502
Size: 495 69 1938
Fragments arranged by size:
1938 495 69
BtgI C'CryG_G
Cuts at: 0 369 483 2036 2411 2502
Size: 369 114 1553 375 91
Fragments arranged by size:
1553 375 369 114 91
BtsI GCAGTG_nn'
Cuts at: 0 1358 1804 1857 2502
Size: 1358 446 53 645
Fragments arranged by size:
1358 645 446 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2018 2053 2502
Size: 2018 35 449
Fragments arranged by size:
2018 449 35
DraIII CAC_nnn'GTG
Cuts at: 0 1744 2439 2502
Size: 1744 695 63
Fragments arranged by size:
1744 695 63
EaeI y'GGCC_r
Cuts at: 0 258 366 1547 2387 2502
Size: 258 108 1181 840 115
Fragments arranged by size:
1181 840 258 115 108
EarI CTCTTCn'nnn_
Cuts at: 0 1210 1672 2191 2502
Size: 1210 462 519 311
Fragments arranged by size:
1210 519 462 311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 665 2502
Size: 665 1837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1095 1222 1231 1257 1396 1653 1717
Size: 198 897 127 9 26 139 257 64
Cuts at: 1717 2172 2319 2502
Size: 455 147 183
Fragments arranged by size:
897 455 257 198 183 147 139 127
64 26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 656 1822 2502
Size: 656 1166 680
Fragments arranged by size:
1166 680 656
EcoO109I rG'GnC_Cy
Cuts at: 0 26 440 592 819 1254 1662 1833 2502
Size: 26 414 152 227 435 408 171 669
Fragments arranged by size:
669 435 414 408 227 171 152 26
EcoRV GAT'ATC
Cuts at: 0 534 2502
Size: 534 1968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 721 2502
Size: 721 1781
HaeII r_GCGC'y
Cuts at: 0 2478 2502
Size: 2478 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 296 328 490 522 612 644 1482 1514
Size: 296 32 162 32 90 32 838 32
Cuts at: 1514 2502
Size: 988
Fragments arranged by size:
988 838 296 162 90 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 181 2502
Size: 181 2321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1994 2502
Size: 817 1177 508
Fragments arranged by size:
1177 817 508
MscI TGG'CCA
Cuts at: 0 260 368 1549 2502
Size: 260 108 1181 953
Fragments arranged by size:
1181 953 260 108
MslI CAynn'nnrTG
Cuts at: 0 1334 1351 1772 2371 2502
Size: 1334 17 421 599 131
Fragments arranged by size:
1334 599 421 131 17
MspA1I CmG'CkG
Cuts at: 0 1242 1529 1761 1980 1995 2010 2466 2502
Size: 1242 287 232 219 15 15 456 36
Fragments arranged by size:
1242 456 287 232 219 36 15 15
NcoI C'CATG_G
Cuts at: 0 483 2502
Size: 483 2019
NdeI CA'TA_TG
Cuts at: 0 948 2502
Size: 948 1554
NspI r_CATG'y
Cuts at: 0 1140 2502
Size: 1140 1362
PasI CC'CwG_GG
Cuts at: 0 1752 2502
Size: 1752 750
PciI A'CATG_T
Cuts at: 0 1136 2502
Size: 1136 1366
PflMI CCAn_nnn'nTGG
Cuts at: 0 1744 2013 2502
Size: 1744 269 489
Fragments arranged by size:
1744 489 269
PfoI T'CCnGG_A
Cuts at: 0 849 1208 1427 2502
Size: 849 359 219 1075
Fragments arranged by size:
1075 849 359 219
PmlI CAC'GTG
Cuts at: 0 447 2502
Size: 447 2055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1424 1456 2502
Size: 1424 32 1046
Fragments arranged by size:
1424 1046 32
PpuMI rG'GwC_Cy
Cuts at: 0 819 2502
Size: 819 1683
PshAI GACnn'nnGTC
Cuts at: 0 2368 2502
Size: 2368 134
PspOMI G'GGCC_C
Cuts at: 0 439 2502
Size: 439 2063
PstI C_TGCA'G
Cuts at: 0 861 2423 2502
Size: 861 1562 79
Fragments arranged by size:
1562 861 79
PvuII CAG'CTG
Cuts at: 0 1529 1761 1980 1995 2010 2466 2502
Size: 1529 232 219 15 15 456 36
Fragments arranged by size:
1529 456 232 219 36 15 15
SapI GCTCTTCn'nnn_
Cuts at: 0 1210 2502
Size: 1210 1292
SbfI CC_TGCA'GG
Cuts at: 0 2423 2502
Size: 2423 79
SfcI C'TryA_G
Cuts at: 0 857 1842 1900 2419 2502
Size: 857 985 58 519 83
Fragments arranged by size:
985 857 519 83 58
SmlI C'TyrA_G
Cuts at: 0 554 629 976 1258 2359 2502
Size: 554 75 347 282 1101 143
Fragments arranged by size:
1101 554 347 282 143 75
SnaBI TAC'GTA
Cuts at: 0 492 2502
Size: 492 2010
StuI AGG'CCT
Cuts at: 0 1817 2502
Size: 1817 685
StyI C'CwwG_G
Cuts at: 0 483 1160 2502
Size: 483 677 1342
Fragments arranged by size:
1342 677 483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 917 1255 2502
Size: 391 526 338 1247
Fragments arranged by size:
1247 526 391 338
TatI w'GTAC_w
Cuts at: 0 1114 2502
Size: 1114 1388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 144 911 943 1732 1764 2502
Size: 112 32 767 32 789 32 738
Fragments arranged by size:
789 767 738 112 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 40 2502
Size: 40 2462
XbaI T'CTAG_A
Cuts at: 0 2452 2502
Size: 2452 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 361 1021 2502
Size: 361 660 1481
Fragments arranged by size:
1481 660 361
ZraI GAC'GTC
Cuts at: 0 525 2502
Size: 525 1977
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BcgI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I
BtgI BtsI CspCI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NdeI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SapI SbfI SfcI SmlI SnaBI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI ZraI
Enzymes that do not cut:
Acc65I AflII AhdI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BglI BglII BmrI
BmtI BsaBI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrGI BssHII BstBI Bsu36I BtgZI ClaI DraI DrdI
EagI EcoICRI EcoRI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PmeI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI SwaI XhoI XmaI XmnI