Restriction map for
pF1KB5786
(Linear) (Six-base) MAPSORT of: KIBB5786.seq Check: 6026 from: 1 to: 2187
KIBB5786.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 288 829 2187
Size: 288 541 1358
Fragments arranged by size:
1358 541 288
AclI AA'CG_TT
Cuts at: 0 855 2187
Size: 855 1332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1088 2187
Size: 285 803 1099
Fragments arranged by size:
1099 803 285
AfeI AGC'GCT
Cuts at: 0 2011 2187
Size: 2011 176
AflIII A'CryG_T
Cuts at: 0 53 629 824 2187
Size: 53 576 195 1363
Fragments arranged by size:
1363 576 195 53
AlwNI CAG_nnn'CTG
Cuts at: 0 661 1495 2187
Size: 661 834 692
Fragments arranged by size:
834 692 661
ApoI r'AATT_y
Cuts at: 0 195 472 604 812 1031 1346 2187
Size: 195 277 132 208 219 315 841
Fragments arranged by size:
841 315 277 219 208 195 132
AvaI C'yCGr_G
Cuts at: 0 1706 1942 2187
Size: 1706 236 245
Fragments arranged by size:
1706 245 236
BamHI G'GATC_C
Cuts at: 0 1938 2187
Size: 1938 249
BanII G_rGCy'C
Cuts at: 0 111 668 2187
Size: 111 557 1519
Fragments arranged by size:
1519 557 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 1304 2187
Size: 1304 883
BclI T'GATC_A
Cuts at: 0 1596 2187
Size: 1596 591
BglII A'GATC_T
Cuts at: 0 466 2187
Size: 466 1721
BlpI GC'TnA_GC
Cuts at: 0 1859 2187
Size: 1859 328
Bme1580I G_kGCm'C
Cuts at: 0 2045 2187
Size: 2045 142
BmgBI CAC'GTC
Cuts at: 0 56 2187
Size: 56 2131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 1592 1932 2187
Size: 1382 210 340 255
Fragments arranged by size:
1382 340 255 210
Bpu10I CC'TnA_GC
Cuts at: 0 102 662 1154 2187
Size: 102 560 492 1033
Fragments arranged by size:
1033 560 492 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 2187
Size: 520 1667
BsaI GGTCTCn'nnnn_
Cuts at: 0 524 2187
Size: 524 1663
BsaHI Gr'CG_yC
Cuts at: 0 72 1979 2187
Size: 72 1907 208
Fragments arranged by size:
1907 208 72
BsaWI w'CCGG_w
Cuts at: 0 2111 2187
Size: 2111 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 2187
Size: 401 30 1756
Fragments arranged by size:
1756 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1190 2187
Size: 1190 997
BseYI C'CCAG_C
Cuts at: 0 1750 2187
Size: 1750 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1234 1658 2080 2187
Size: 158 1076 424 422 107
Fragments arranged by size:
1076 424 422 158 107
BsiHKAI G_wGCw'C
Cuts at: 0 111 668 2187
Size: 111 557 1519
Fragments arranged by size:
1519 557 111
BsmI GAATG_Cn'
Cuts at: 0 1425 2187
Size: 1425 762
Bsp1286I G_dGCh'C
Cuts at: 0 111 668 2045 2187
Size: 111 557 1377 142
Fragments arranged by size:
1377 557 142 111
BspHI T'CATG_A
Cuts at: 0 902 2187
Size: 902 1285
BsrBI CCG'CTC
Cuts at: 0 101 2187
Size: 101 2086
BsrDI GCAATG_nn'
Cuts at: 0 16 606 1854 2187
Size: 16 590 1248 333
Fragments arranged by size:
1248 590 333 16
BssSI C'ACGA_G
Cuts at: 0 2056 2187
Size: 2056 131
BstBI TT'CG_AA
Cuts at: 0 356 2187
Size: 356 1831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2038 2187
Size: 2038 149
BstYI r'GATC_y
Cuts at: 0 466 1938 2187
Size: 466 1472 249
Fragments arranged by size:
1472 466 249
BtgI C'CryG_G
Cuts at: 0 1695 2031 2187
Size: 1695 336 156
Fragments arranged by size:
1695 336 156
BtsI GCAGTG_nn'
Cuts at: 0 1176 1316 1377 1536 1673 2068 2187
Size: 1176 140 61 159 137 395 119
Fragments arranged by size:
1176 395 159 140 137 119 61
DraI TTT'AAA
Cuts at: 0 329 2187
Size: 329 1858
DraIII CAC_nnn'GTG
Cuts at: 0 1212 1560 2187
Size: 1212 348 627
Fragments arranged by size:
1212 627 348
EaeI y'GGCC_r
Cuts at: 0 1191 1698 2187
Size: 1191 507 489
Fragments arranged by size:
1191 507 489
EarI CTCTTCn'nnn_
Cuts at: 0 304 673 2026 2187
Size: 304 369 1353 161
Fragments arranged by size:
1353 369 304 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1456 2187
Size: 1456 731
EcoICRI GAG'CTC
Cuts at: 0 109 666 2187
Size: 109 557 1521
Fragments arranged by size:
1521 557 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1088 1382 1592 1932 2187
Size: 285 803 294 210 340 255
Fragments arranged by size:
803 340 294 285 255 210
EcoO109I rG'GnC_Cy
Cuts at: 0 11 2187
Size: 11 2176
EcoRI G'AATT_C
Cuts at: 0 812 2187
Size: 812 1375
EcoRV GAT'ATC
Cuts at: 0 2126 2187
Size: 2126 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1060 1244 2187
Size: 1060 184 943
Fragments arranged by size:
1060 943 184
HaeII r_GCGC'y
Cuts at: 0 2013 2187
Size: 2013 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 510 542 1158 1190 1540 1572
Size: 315 32 163 32 616 32 350 32
Cuts at: 1572 2187
Size: 615
Fragments arranged by size:
616 615 350 315 163 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1359 1955 2187
Size: 304 1055 596 232
Fragments arranged by size:
1055 596 304 232
MscI TGG'CCA
Cuts at: 0 1193 2187
Size: 1193 994
MslI CAynn'nnrTG
Cuts at: 0 65 2036 2187
Size: 65 1971 151
Fragments arranged by size:
1971 151 65
MspA1I CmG'CkG
Cuts at: 0 1546 2187
Size: 1546 641
NspI r_CATG'y
Cuts at: 0 828 2187
Size: 828 1359
PciI A'CATG_T
Cuts at: 0 824 2187
Size: 824 1363
PfoI T'CCnGG_A
Cuts at: 0 1354 2187
Size: 1354 833
PpuMI rG'GwC_Cy
Cuts at: 0 11 2187
Size: 11 2176
PstI C_TGCA'G
Cuts at: 0 495 1323 2187
Size: 495 828 864
Fragments arranged by size:
864 828 495
PvuII CAG'CTG
Cuts at: 0 1546 2187
Size: 1546 641
SacI G_AGCT'C
Cuts at: 0 111 668 2187
Size: 111 557 1519
Fragments arranged by size:
1519 557 111
SapI GCTCTTCn'nnn_
Cuts at: 0 304 673 2026 2187
Size: 304 369 1353 161
Fragments arranged by size:
1353 369 304 161
ScaI AGT'ACT
Cuts at: 0 1188 2187
Size: 1188 999
SfcI C'TryA_G
Cuts at: 0 125 165 491 830 1251 1319 1541 2187
Size: 125 40 326 339 421 68 222 646
Fragments arranged by size:
646 421 339 326 222 125 68 40
SmaI CCC'GGG
Cuts at: 0 1708 1944 2187
Size: 1708 236 243
Fragments arranged by size:
1708 243 236
SmlI C'TyrA_G
Cuts at: 0 535 2187
Size: 535 1652
SpeI A'CTAG_T
Cuts at: 0 735 1927 2187
Size: 735 1192 260
Fragments arranged by size:
1192 735 260
SspI AAT'ATT
Cuts at: 0 2154 2187
Size: 2154 33
TatI w'GTAC_w
Cuts at: 0 1186 2187
Size: 1186 1001
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1350 1382 2187
Size: 1350 32 805
Fragments arranged by size:
1350 805 32
XbaI T'CTAG_A
Cuts at: 0 891 2187
Size: 891 1296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 58 1932 2187
Size: 58 1874 255
Fragments arranged by size:
1874 255 58
XmaI C'CCGG_G
Cuts at: 0 1706 1942 2187
Size: 1706 236 245
Fragments arranged by size:
1706 245 236
XmnI GAAnn'nnTTC
Cuts at: 0 352 845 1031 1101 1224 2187
Size: 352 493 186 70 123 963
Fragments arranged by size:
963 493 352 186 123 70
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AlwNI ApoI AvaI
BamHI BanII BciVI BclI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI
BssSI BstBI BstXI BstYI BtgI BtsI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I MmeI MscI MslI MspA1I NspI
PciI PfoI PpuMI PstI PvuII SacI SapI ScaI
SfcI SmaI SmlI SpeI SspI TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbsI BbvCI BcgI BfrBI BglI BmrI
BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI
BspMI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EcoNI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI StuI StyI SwaI TaqII Tth111I XhoI
ZraI