Restriction map for
pF1KB5777
(Linear) (Six-base) MAPSORT of: KIBB5777.seq Check: 8967 from: 1 to: 2466
KIBB5777.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 2466
Size: 83 2383
AflII C'TTAA_G
Cuts at: 0 456 2466
Size: 456 2010
AhdI GACnn_n'nnGTC
Cuts at: 0 292 2466
Size: 292 2174
AleI CACnn'nnGTG
Cuts at: 0 1048 1558 2466
Size: 1048 510 908
Fragments arranged by size:
1048 908 510
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 2466
Size: 1280 32 1154
Fragments arranged by size:
1280 1154 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1374 2011 2466
Size: 1374 637 455
Fragments arranged by size:
1374 637 455
ApoI r'AATT_y
Cuts at: 0 232 540 743 761 1183 2152 2466
Size: 232 308 203 18 422 969 314
Fragments arranged by size:
969 422 314 308 232 203 18
BanII G_rGCy'C
Cuts at: 0 391 2413 2466
Size: 391 2022 53
Fragments arranged by size:
2022 391 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 1006 2466
Size: 128 878 1460
Fragments arranged by size:
1460 878 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 330 364 2466
Size: 330 34 2102
Fragments arranged by size:
2102 330 34
BfrBI ATG'CAT
Cuts at: 0 1353 2466
Size: 1353 1113
BglII A'GATC_T
Cuts at: 0 408 2424 2466
Size: 408 2016 42
Fragments arranged by size:
2016 408 42
BlpI GC'TnA_GC
Cuts at: 0 2412 2466
Size: 2412 54
Bme1580I G_kGCm'C
Cuts at: 0 650 2466
Size: 650 1816
BmrI ACTGGGnnnn_n'
Cuts at: 0 2177 2466
Size: 2177 289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 1988 2466
Size: 1660 328 478
Fragments arranged by size:
1660 478 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1762 2466
Size: 196 1566 704
Fragments arranged by size:
1566 704 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 1825 2466
Size: 1825 641
BsaBI GATnn'nnATC
Cuts at: 0 296 332 2466
Size: 296 36 2134
Fragments arranged by size:
2134 296 36
BsaWI w'CCGG_w
Cuts at: 0 32 41 101 685 2466
Size: 32 9 60 584 1781
Fragments arranged by size:
1781 584 60 32 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1986 2016 2466
Size: 1986 30 450
Fragments arranged by size:
1986 450 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 381 580 2466
Size: 381 199 1886
Fragments arranged by size:
1886 381 199
BseYI C'CCAG_C
Cuts at: 0 174 1977 2045 2310 2466
Size: 174 1803 68 265 156
Fragments arranged by size:
1803 265 174 156 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 1816 2466
Size: 423 1393 650
Fragments arranged by size:
1393 650 423
BsiHKAI G_wGCw'C
Cuts at: 0 2004 2466
Size: 2004 462
BsmI GAATG_Cn'
Cuts at: 0 1754 2384 2466
Size: 1754 630 82
Fragments arranged by size:
1754 630 82
Bsp1286I G_dGCh'C
Cuts at: 0 391 650 2004 2413 2466
Size: 391 259 1354 409 53
Fragments arranged by size:
1354 409 391 259 53
BspEI T'CCGG_A
Cuts at: 0 32 41 685 2466
Size: 32 9 644 1781
Fragments arranged by size:
1781 644 32 9
BspHI T'CATG_A
Cuts at: 0 305 2466
Size: 305 2161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 726 2466
Size: 726 1740
BsrDI GCAATG_nn'
Cuts at: 0 272 1100 2423 2466
Size: 272 828 1323 43
Fragments arranged by size:
1323 828 272 43
BsrGI T'GTAC_A
Cuts at: 0 1005 1376 2466
Size: 1005 371 1090
Fragments arranged by size:
1090 1005 371
BssSI C'ACGA_G
Cuts at: 0 1565 2466
Size: 1565 901
BstAPI GCAn_nnn'nTGC
Cuts at: 0 478 820 1198 2373 2466
Size: 478 342 378 1175 93
Fragments arranged by size:
1175 478 378 342 93
BstYI r'GATC_y
Cuts at: 0 408 2424 2466
Size: 408 2016 42
Fragments arranged by size:
2016 408 42
BtgI C'CryG_G
Cuts at: 0 561 2466
Size: 561 1905
BtsI GCAGTG_nn'
Cuts at: 0 1580 2466
Size: 1580 886
DraI TTT'AAA
Cuts at: 0 668 1188 2466
Size: 668 520 1278
Fragments arranged by size:
1278 668 520
DraIII CAC_nnn'GTG
Cuts at: 0 478 2466
Size: 478 1988
EaeI y'GGCC_r
Cuts at: 0 1467 2466
Size: 1467 999
EarI CTCTTCn'nnn_
Cuts at: 0 1472 1478 2092 2466
Size: 1472 6 614 374
Fragments arranged by size:
1472 614 374 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1660 1988 2466
Size: 83 1577 328 478
Fragments arranged by size:
1577 478 328 83
EcoRI G'AATT_C
Cuts at: 0 2152 2466
Size: 2152 314
EcoRV GAT'ATC
Cuts at: 0 2042 2466
Size: 2042 424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1171 1405 2157 2466
Size: 1171 234 752 309
Fragments arranged by size:
1171 752 309 234
FspI TGC'GCA
Cuts at: 0 629 2466
Size: 629 1837
HaeII r_GCGC'y
Cuts at: 0 1374 2466
Size: 1374 1092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 278 310 883 902 915 934
Size: 62 32 184 32 573 19 13 19
Cuts at: 934 1186 1218 2466
Size: 252 32 1248
Fragments arranged by size:
1248 573 252 184 62 32 32 32
19 19 13
HindIII A'AGCT_T
Cuts at: 0 214 640 1594 2389 2466
Size: 214 426 954 795 77
Fragments arranged by size:
954 795 426 214 77
MfeI C'AATT_G
Cuts at: 0 1046 2466
Size: 1046 1420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 182 470 1496 1948 2042 2237 2466
Size: 121 61 288 1026 452 94 195 229
Fragments arranged by size:
1026 452 288 229 195 121 94 61
MscI TGG'CCA
Cuts at: 0 1469 2466
Size: 1469 997
MslI CAynn'nnrTG
Cuts at: 0 135 598 1048 1264 1558 1666 2466
Size: 135 463 450 216 294 108 800
Fragments arranged by size:
800 463 450 294 216 135 108
MspA1I CmG'CkG
Cuts at: 0 2008 2277 2373 2466
Size: 2008 269 96 93
Fragments arranged by size:
2008 269 96 93
NcoI C'CATG_G
Cuts at: 0 561 2466
Size: 561 1905
NsiI A_TGCA'T
Cuts at: 0 1355 2466
Size: 1355 1111
NspI r_CATG'y
Cuts at: 0 174 624 1357 2466
Size: 174 450 733 1109
Fragments arranged by size:
1109 733 450 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 1247 1849 2466
Size: 1247 602 617
Fragments arranged by size:
1247 617 602
PstI C_TGCA'G
Cuts at: 0 1224 2106 2259 2282 2466
Size: 1224 882 153 23 184
Fragments arranged by size:
1224 882 184 153 23
PvuII CAG'CTG
Cuts at: 0 2008 2277 2373 2466
Size: 2008 269 96 93
Fragments arranged by size:
2008 269 96 93
SapI GCTCTTCn'nnn_
Cuts at: 0 1478 2466
Size: 1478 988
SfcI C'TryA_G
Cuts at: 0 581 1220 2102 2255 2278 2466
Size: 581 639 882 153 23 188
Fragments arranged by size:
882 639 581 188 153 23
SmlI C'TyrA_G
Cuts at: 0 211 456 1741 2466
Size: 211 245 1285 725
Fragments arranged by size:
1285 725 245 211
SpeI A'CTAG_T
Cuts at: 0 838 2466
Size: 838 1628
SphI G_CATG'C
Cuts at: 0 1357 2466
Size: 1357 1109
SspI AAT'ATT
Cuts at: 0 969 2466
Size: 969 1497
StuI AGG'CCT
Cuts at: 0 2209 2466
Size: 2209 257
StyI C'CwwG_G
Cuts at: 0 561 2466
Size: 561 1905
TatI w'GTAC_w
Cuts at: 0 1005 1026 1376 1615 2466
Size: 1005 21 350 239 851
Fragments arranged by size:
1005 851 350 239 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 933 1881 2466
Size: 933 948 585
Fragments arranged by size:
948 933 585
Enzymes that do cut:
AcuI AflII AhdI AleI AloI AlwNI ApoI BanII
BbsI BcgI BfrBI BglII BlpI Bme1580I BmrI BpmI
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI
BssSI BstAPI BstYI BtgI BtsI DraI DraIII EaeI
EarI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PflMI PstI PvuII SapI SfcI SmlI SpeI
SphI SspI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BglI
BmgBI BmtI BplI Bpu10I BsaAI BsaHI BsiEI BsiWI
BsmBI BsrBI BsrFI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DrdI EagI EciI EcoICRI
EcoNI EcoO109I FseI FspAI HincII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI