Restriction map for
pF1KB5776
(Linear) (Six-base) MAPSORT of: KIBB5776.seq Check: 8149 from: 1 to: 2298
KIBB5776.orf
With 182 enzymes: SgfI *
July 22, 2009 17:02 ..
AccI GT'mk_AC
Cuts at: 0 1326 1412 2298
Size: 1326 86 886
Fragments arranged by size:
1326 886 86
Acc65I G'GTAC_C
Cuts at: 0 504 2298
Size: 504 1794
AclI AA'CG_TT
Cuts at: 0 721 2298
Size: 721 1577
AflIII A'CryG_T
Cuts at: 0 787 1527 2298
Size: 787 740 771
Fragments arranged by size:
787 771 740
AleI CACnn'nnGTG
Cuts at: 0 427 834 2177 2298
Size: 427 407 1343 121
Fragments arranged by size:
1343 427 407 121
AlwNI CAG_nnn'CTG
Cuts at: 0 51 928 2298
Size: 51 877 1370
Fragments arranged by size:
1370 877 51
ApaI G_GGCC'C
Cuts at: 0 983 2298
Size: 983 1315
ApoI r'AATT_y
Cuts at: 0 199 596 799 986 1639 2126 2168 2298
Size: 199 397 203 187 653 487 42 130
Fragments arranged by size:
653 487 397 203 199 187 130 42
AseI AT'TA_AT
Cuts at: 0 2074 2298
Size: 2074 224
AvaI C'yCGr_G
Cuts at: 0 73 1335 2298
Size: 73 1262 963
Fragments arranged by size:
1262 963 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 488 521 2298
Size: 488 33 1777
Fragments arranged by size:
1777 488 33
BamHI G'GATC_C
Cuts at: 0 1843 2298
Size: 1843 455
BanI G'GyrC_C
Cuts at: 0 82 88 94 504 1860 2298
Size: 82 6 6 410 1356 438
Fragments arranged by size:
1356 438 410 82 6 6
BanII G_rGCy'C
Cuts at: 0 74 983 1603 2298
Size: 74 909 620 695
Fragments arranged by size:
909 695 620 74
BbeI G_GCGC'C
Cuts at: 0 86 92 98 2298
Size: 86 6 6 2200
Fragments arranged by size:
2200 86 6 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 468 1304 1763 2298
Size: 468 836 459 535
Fragments arranged by size:
836 535 468 459
BbvCI CC'TCA_GC
Cuts at: 0 1265 1475 2298
Size: 1265 210 823
Fragments arranged by size:
1265 823 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 981 2298
Size: 947 34 1317
Fragments arranged by size:
1317 947 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 345 2008 2298
Size: 345 1663 290
Fragments arranged by size:
1663 345 290
BclI T'GATC_A
Cuts at: 0 1649 2298
Size: 1649 649
BfrBI ATG'CAT
Cuts at: 0 1921 2298
Size: 1921 377
BglI GCCn_nnn'nGGC
Cuts at: 0 109 2298
Size: 109 2189
BglII A'GATC_T
Cuts at: 0 2028 2298
Size: 2028 270
BlpI GC'TnA_GC
Cuts at: 0 10 2298
Size: 10 2288
Bme1580I G_kGCm'C
Cuts at: 0 983 2298
Size: 983 1315
BmrI ACTGGGnnnn_n'
Cuts at: 0 1581 2298
Size: 1581 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 674 1241 1587 2009 2298
Size: 256 418 567 346 422 289
Fragments arranged by size:
567 422 418 346 289 256
Bpu10I CC'TnA_GC
Cuts at: 0 732 918 1265 1475 2298
Size: 732 186 347 210 823
Fragments arranged by size:
823 732 347 210 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1714 2298
Size: 1714 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 1073 1145 1627 1718 2103 2298
Size: 1073 72 482 91 385 195
Fragments arranged by size:
1073 482 385 195 91 72
BsaBI GATnn'nnATC
Cuts at: 0 1052 1089 1395 1889 2298
Size: 1052 37 306 494 409
Fragments arranged by size:
1052 494 409 306 37
BsaHI Gr'CG_yC
Cuts at: 0 83 89 95 913 2298
Size: 83 6 6 818 1385
Fragments arranged by size:
1385 818 83 6 6
BsaWI w'CCGG_w
Cuts at: 0 129 2298
Size: 129 2169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 512 542 2037 2067 2298
Size: 124 30 358 30 1495 30 231
Fragments arranged by size:
1495 358 231 124 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 2298
Size: 148 2150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1664 2298
Size: 1664 634
BsmI GAATG_Cn'
Cuts at: 0 1883 2298
Size: 1883 415
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1007 2298
Size: 1007 1291
Bsp1286I G_dGCh'C
Cuts at: 0 74 983 1603 2298
Size: 74 909 620 695
Fragments arranged by size:
909 695 620 74
BspEI T'CCGG_A
Cuts at: 0 129 2298
Size: 129 2169
BspHI T'CATG_A
Cuts at: 0 1752 2298
Size: 1752 546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 516 2298
Size: 516 1782
BsrBI CCG'CTC
Cuts at: 0 14 2156 2298
Size: 14 2142 142
Fragments arranged by size:
2142 142 14
BsrDI GCAATG_nn'
Cuts at: 0 2158 2298
Size: 2158 140
BsrFI r'CCGG_y
Cuts at: 0 79 85 91 2298
Size: 79 6 6 2207
Fragments arranged by size:
2207 79 6 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 928 2298
Size: 928 1370
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 626 2298
Size: 429 197 1672
Fragments arranged by size:
1672 429 197
BstYI r'GATC_y
Cuts at: 0 1390 1843 2028 2298
Size: 1390 453 185 270
Fragments arranged by size:
1390 453 270 185
BtgI C'CryG_G
Cuts at: 0 98 2298
Size: 98 2200
BtsI GCAGTG_nn'
Cuts at: 0 1448 2184 2298
Size: 1448 736 114
Fragments arranged by size:
1448 736 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 186 221 2298
Size: 186 35 2077
Fragments arranged by size:
2077 186 35
EarI CTCTTCn'nnn_
Cuts at: 0 111 378 902 1549 2062 2298
Size: 111 267 524 647 513 236
Fragments arranged by size:
647 524 513 267 236 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 674 1241 1587 2009 2298
Size: 256 418 567 346 422 289
Fragments arranged by size:
567 422 418 346 289 256
EcoO109I rG'GnC_Cy
Cuts at: 0 186 980 1261 1817 2298
Size: 186 794 281 556 481
Fragments arranged by size:
794 556 481 281 186
EcoRI G'AATT_C
Cuts at: 0 596 2298
Size: 596 1702
HaeII r_GCGC'y
Cuts at: 0 10 86 92 98 2298
Size: 10 76 6 6 2200
Fragments arranged by size:
2200 76 10 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 512 544 549 554 581 586 1072 1104
Size: 512 32 5 5 27 5 486 32
Cuts at: 1104 1162 1194 2037 2069 2298
Size: 58 32 843 32 229
Fragments arranged by size:
843 512 486 229 58 32 32 32
32 27 5 5 5
HincII GTy'rAC
Cuts at: 0 167 2298
Size: 167 2131
KasI G'GCGC_C
Cuts at: 0 82 88 94 2298
Size: 82 6 6 2204
Fragments arranged by size:
2204 82 6 6
KpnI G_GTAC'C
Cuts at: 0 508 2298
Size: 508 1790
MfeI C'AATT_G
Cuts at: 0 620 2298
Size: 620 1678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 659 1062 2298
Size: 300 359 403 1236
Fragments arranged by size:
1236 403 359 300
MslI CAynn'nnrTG
Cuts at: 0 427 834 1433 1876 2177 2298
Size: 427 407 599 443 301 121
Fragments arranged by size:
599 443 427 407 301 121
MspA1I CmG'CkG
Cuts at: 0 100 779 2298
Size: 100 679 1519
Fragments arranged by size:
1519 679 100
NaeI GCC'GGC
Cuts at: 0 81 87 93 2298
Size: 81 6 6 2205
Fragments arranged by size:
2205 81 6 6
NarI GG'CG_CC
Cuts at: 0 83 89 95 2298
Size: 83 6 6 2203
Fragments arranged by size:
2203 83 6 6
NgoMIV G'CCGG_C
Cuts at: 0 79 85 91 2298
Size: 79 6 6 2207
Fragments arranged by size:
2207 79 6 6
NsiI A_TGCA'T
Cuts at: 0 1923 2298
Size: 1923 375
NspI r_CATG'y
Cuts at: 0 670 791 1531 2298
Size: 670 121 740 767
Fragments arranged by size:
767 740 670 121
PciI A'CATG_T
Cuts at: 0 787 1527 2298
Size: 787 740 771
Fragments arranged by size:
787 771 740
PflMI CCAn_nnn'nTGG
Cuts at: 0 1853 2298
Size: 1853 445
PfoI T'CCnGG_A
Cuts at: 0 1257 2298
Size: 1257 1041
PpuMI rG'GwC_Cy
Cuts at: 0 1261 1817 2298
Size: 1261 556 481
Fragments arranged by size:
1261 556 481
PshAI GACnn'nnGTC
Cuts at: 0 749 2298
Size: 749 1549
PsiI TTA'TAA
Cuts at: 0 797 2298
Size: 797 1501
PspOMI G'GGCC_C
Cuts at: 0 979 2298
Size: 979 1319
PspXI vC'TCGA_Gb
Cuts at: 0 1335 2298
Size: 1335 963
SacII CC_GC'GG
Cuts at: 0 101 2298
Size: 101 2197
SanDI GG'GwC_CC
Cuts at: 0 1817 2298
Size: 1817 481
ScaI AGT'ACT
Cuts at: 0 1511 2298
Size: 1511 787
SfcI C'TryA_G
Cuts at: 0 1793 2298
Size: 1793 505
SfoI GGC'GCC
Cuts at: 0 84 90 96 2298
Size: 84 6 6 2202
Fragments arranged by size:
2202 84 6 6
SgrAI Cr'CCGG_yG
Cuts at: 0 85 91 2298
Size: 85 6 2207
Fragments arranged by size:
2207 85 6
SmlI C'TyrA_G
Cuts at: 0 1335 1729 2298
Size: 1335 394 569
Fragments arranged by size:
1335 569 394
SspI AAT'ATT
Cuts at: 0 1134 2298
Size: 1134 1164
StuI AGG'CCT
Cuts at: 0 241 2298
Size: 241 2057
StyI C'CwwG_G
Cuts at: 0 1155 2298
Size: 1155 1143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 908 1097 2274 2298
Size: 908 189 1177 24
Fragments arranged by size:
1177 908 189 24
TatI w'GTAC_w
Cuts at: 0 817 1509 2298
Size: 817 692 789
Fragments arranged by size:
817 789 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 124 156 1019 1051 2298
Size: 124 32 863 32 1247
Fragments arranged by size:
1247 863 124 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1162 1854 2298
Size: 1162 692 444
Fragments arranged by size:
1162 692 444
XhoI C'TCGA_G
Cuts at: 0 1335 2298
Size: 1335 963
Enzymes that do cut:
AccI Acc65I AclI AflIII AleI AlwNI ApaI ApoI
AseI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
BtgI BtsI CspCI EarI Eco57MI EcoO109I EcoRI HaeII
Hin4I HincII KasI KpnI MfeI MmeI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI SacII SanDI ScaI
SfcI SfoI SgrAI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AcuI AfeI AflII AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvrII BmgBI BmtI BplI
BsaAI BseYI BsiEI BsiHKAI BsiWI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HindIII HpaI MluI MscI NcoI
NdeI NheI NotI NruI PacI PasI PmeI PmlI
PpiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XmaI XmnI ZraI