Restriction map for
pF1KB5711
(Linear) (Six-base) MAPSORT of: KIBB5711.seq Check: 7589 from: 1 to: 2355
KIBB5711.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 0 575 2082 2355
Size: 575 1507 273
Fragments arranged by size:
1507 575 273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1539 2355
Size: 1539 816
AfeI AGC'GCT
Cuts at: 0 371 2355
Size: 371 1984
AflIII A'CryG_T
Cuts at: 0 2321 2355
Size: 2321 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2213 2355
Size: 2213 142
AlwNI CAG_nnn'CTG
Cuts at: 0 745 2224 2355
Size: 745 1479 131
Fragments arranged by size:
1479 745 131
ApoI r'AATT_y
Cuts at: 0 425 454 1441 2066 2125 2355
Size: 425 29 987 625 59 230
Fragments arranged by size:
987 625 425 230 59 29
AvaI C'yCGr_G
Cuts at: 0 403 2355
Size: 403 1952
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1126 1159 2355
Size: 1126 33 1196
Fragments arranged by size:
1196 1126 33
BanII G_rGCy'C
Cuts at: 0 366 404 1121 1827 1910 2355
Size: 366 38 717 706 83 445
Fragments arranged by size:
717 706 445 366 83 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1899 2355
Size: 1899 456
BclI T'GATC_A
Cuts at: 0 2265 2355
Size: 2265 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 2301 2355
Size: 311 1990 54
Fragments arranged by size:
1990 311 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 2045 2355
Size: 190 1855 310
Fragments arranged by size:
1855 310 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 350 1367 2355
Size: 72 278 1017 988
Fragments arranged by size:
1017 988 278 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 1453 2355
Size: 1453 902
BsaBI GATnn'nnATC
Cuts at: 0 2159 2355
Size: 2159 196
BsaWI w'CCGG_w
Cuts at: 0 1649 2355
Size: 1649 706
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 2355
Size: 391 30 1934
Fragments arranged by size:
1934 391 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 1993 2162 2355
Size: 352 1641 169 193
Fragments arranged by size:
1641 352 193 169
BseYI C'CCAG_C
Cuts at: 0 143 1481 2355
Size: 143 1338 874
Fragments arranged by size:
1338 874 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1991 2355
Size: 1991 364
BsiHKAI G_wGCw'C
Cuts at: 0 366 2172 2355
Size: 366 1806 183
Fragments arranged by size:
1806 366 183
Bsp1286I G_dGCh'C
Cuts at: 0 366 404 1121 1827 1910 2172 2355
Size: 366 38 717 706 83 262 183
Fragments arranged by size:
717 706 366 262 183 83 38
BspEI T'CCGG_A
Cuts at: 0 1649 2355
Size: 1649 706
BspHI T'CATG_A
Cuts at: 0 1203 2355
Size: 1203 1152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1482 1786 1812 2355
Size: 1482 304 26 543
Fragments arranged by size:
1482 543 304 26
BsrDI GCAATG_nn'
Cuts at: 0 1367 1788 2355
Size: 1367 421 567
Fragments arranged by size:
1367 567 421
BstXI CCAn_nnnn'nTGG
Cuts at: 0 327 459 1317 2355
Size: 327 132 858 1038
Fragments arranged by size:
1038 858 327 132
BstYI r'GATC_y
Cuts at: 0 1702 2355
Size: 1702 653
BtgI C'CryG_G
Cuts at: 0 395 500 1448 2355
Size: 395 105 948 907
Fragments arranged by size:
948 907 395 105
DraI TTT'AAA
Cuts at: 0 2094 2307 2355
Size: 2094 213 48
Fragments arranged by size:
2094 213 48
EaeI y'GGCC_r
Cuts at: 0 842 1403 2334 2355
Size: 842 561 931 21
Fragments arranged by size:
931 842 561 21
EarI CTCTTCn'nnn_
Cuts at: 0 1137 1386 2355
Size: 1137 249 969
Fragments arranged by size:
1137 969 249
EcoICRI GAG'CTC
Cuts at: 0 364 2355
Size: 364 1991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1539 2045 2355
Size: 190 1349 506 310
Fragments arranged by size:
1349 506 310 190
EcoO109I rG'GnC_Cy
Cuts at: 0 125 506 2296 2355
Size: 125 381 1790 59
Fragments arranged by size:
1790 381 125 59
EcoRV GAT'ATC
Cuts at: 0 435 2355
Size: 435 1920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1927 2355
Size: 1927 428
HaeII r_GCGC'y
Cuts at: 0 373 2355
Size: 373 1982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 391 423 1050 1082 1359 1391
Size: 315 32 44 32 627 32 277 32
Cuts at: 1391 1516 1548 1687 1719 2355
Size: 125 32 139 32 636
Fragments arranged by size:
636 627 315 277 139 125 44 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 765 942 2083 2355
Size: 765 177 1141 272
Fragments arranged by size:
1141 765 272 177
HindIII A'AGCT_T
Cuts at: 0 208 2355
Size: 208 2147
MfeI C'AATT_G
Cuts at: 0 1241 2355
Size: 1241 1114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1139 2355
Size: 389 750 1216
Fragments arranged by size:
1216 750 389
MscI TGG'CCA
Cuts at: 0 844 1405 2336 2355
Size: 844 561 931 19
Fragments arranged by size:
931 844 561 19
MslI CAynn'nnrTG
Cuts at: 0 218 1741 2355
Size: 218 1523 614
Fragments arranged by size:
1523 614 218
MspA1I CmG'CkG
Cuts at: 0 143 2355
Size: 143 2212
NcoI C'CATG_G
Cuts at: 0 1448 2355
Size: 1448 907
NdeI CA'TA_TG
Cuts at: 0 552 2196 2355
Size: 552 1644 159
Fragments arranged by size:
1644 552 159
NspI r_CATG'y
Cuts at: 0 2325 2355
Size: 2325 30
PciI A'CATG_T
Cuts at: 0 2321 2355
Size: 2321 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 1537 2355
Size: 1537 818
PfoI T'CCnGG_A
Cuts at: 0 127 2355
Size: 127 2228
PpuMI rG'GwC_Cy
Cuts at: 0 125 506 2296 2355
Size: 125 381 1790 59
Fragments arranged by size:
1790 381 125 59
PshAI GACnn'nnGTC
Cuts at: 0 625 1465 2355
Size: 625 840 890
Fragments arranged by size:
890 840 625
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2263 2295 2355
Size: 2263 32 60
Fragments arranged by size:
2263 60 32
PstI C_TGCA'G
Cuts at: 0 38 2192 2355
Size: 38 2154 163
Fragments arranged by size:
2154 163 38
PvuII CAG'CTG
Cuts at: 0 143 2355
Size: 143 2212
SacI G_AGCT'C
Cuts at: 0 366 2355
Size: 366 1989
SalI G'TCGA_C
Cuts at: 0 2081 2355
Size: 2081 274
SfcI C'TryA_G
Cuts at: 0 34 576 827 1193 2188 2355
Size: 34 542 251 366 995 167
Fragments arranged by size:
995 542 366 251 167 34
SmlI C'TyrA_G
Cuts at: 0 87 365 1382 2355
Size: 87 278 1017 973
Fragments arranged by size:
1017 973 278 87
SspI AAT'ATT
Cuts at: 0 1278 1947 2355
Size: 1278 669 408
Fragments arranged by size:
1278 669 408
StuI AGG'CCT
Cuts at: 0 346 1792 2355
Size: 346 1446 563
Fragments arranged by size:
1446 563 346
StyI C'CwwG_G
Cuts at: 0 1448 2355
Size: 1448 907
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 2355
Size: 135 2220
TatI w'GTAC_w
Cuts at: 0 893 1816 2348 2355
Size: 893 923 532 7
Fragments arranged by size:
923 893 532 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 393 2355
Size: 324 69 1962
Fragments arranged by size:
1962 324 69
XmnI GAAnn'nnTTC
Cuts at: 0 157 2355
Size: 157 2198
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AlwNI ApoI AvaI
BaeI BanII BbsI BclI BmrI BpmI BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrDI BstXI BstYI BtgI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI NdeI NspI PciI PflMI PfoI PpuMI PshAI
PsrI PstI PvuII SacI SalI SfcI SmlI SspI
StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaHI
BsiEI BsiWI BsmI BsmBI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI
FseI FspI FspAI HpaI KasI KpnI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI