Restriction map for
pF1KB5706
(Linear) (Six-base) MAPSORT of: KIBB5706.seq Check: 5264 from: 1 to: 2151
KIBB5706.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 1500 2151
Size: 1500 651
AflIII A'CryG_T
Cuts at: 0 651 2151
Size: 651 1500
AlwNI CAG_nnn'CTG
Cuts at: 0 200 849 2151
Size: 200 649 1302
Fragments arranged by size:
1302 649 200
ApaI G_GGCC'C
Cuts at: 0 1458 1989 2151
Size: 1458 531 162
Fragments arranged by size:
1458 531 162
ApoI r'AATT_y
Cuts at: 0 1147 1922 2151
Size: 1147 775 229
Fragments arranged by size:
1147 775 229
AvaI C'yCGr_G
Cuts at: 0 1388 2151
Size: 1388 763
BanI G'GyrC_C
Cuts at: 0 708 2151
Size: 708 1443
BanII G_rGCy'C
Cuts at: 0 1458 1989 2151
Size: 1458 531 162
Fragments arranged by size:
1458 531 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 2151
Size: 160 1991
BclI T'GATC_A
Cuts at: 0 539 2151
Size: 539 1612
BlpI GC'TnA_GC
Cuts at: 0 1655 2151
Size: 1655 496
Bme1580I G_kGCm'C
Cuts at: 0 1458 1989 2151
Size: 1458 531 162
Fragments arranged by size:
1458 531 162
BmgBI CAC'GTC
Cuts at: 0 652 2151
Size: 652 1499
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 179 888 1675 1825 2151
Size: 171 8 709 787 150 326
Fragments arranged by size:
787 709 326 171 150 8
BmtI G_CTAG'C
Cuts at: 0 1663 1999 2151
Size: 1663 336 152
Fragments arranged by size:
1663 336 152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 803 2151
Size: 803 1348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1463 1781 2151
Size: 579 884 318 370
Fragments arranged by size:
884 579 370 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 713 922 2151
Size: 353 360 209 1229
Fragments arranged by size:
1229 360 353 209
BsaBI GATnn'nnATC
Cuts at: 0 1598 2151
Size: 1598 553
BsaWI w'CCGG_w
Cuts at: 0 1674 2151
Size: 1674 477
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2084 2114 2151
Size: 2084 30 37
Fragments arranged by size:
2084 37 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 704 716 1862 2151
Size: 704 12 1146 289
Fragments arranged by size:
1146 704 289 12
BseYI C'CCAG_C
Cuts at: 0 858 1302 2151
Size: 858 444 849
Fragments arranged by size:
858 849 444
BsiHKAI G_wGCw'C
Cuts at: 0 2130 2151
Size: 2130 21
BsmI GAATG_Cn'
Cuts at: 0 107 648 1531 2151
Size: 107 541 883 620
Fragments arranged by size:
883 620 541 107
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2 2151
Size: 2 2149
Bsp1286I G_dGCh'C
Cuts at: 0 1458 1989 2130 2151
Size: 1458 531 141 21
Fragments arranged by size:
1458 531 141 21
BspEI T'CCGG_A
Cuts at: 0 1674 2151
Size: 1674 477
BspHI T'CATG_A
Cuts at: 0 2112 2151
Size: 2112 39
BsrGI T'GTAC_A
Cuts at: 0 209 1899 2151
Size: 209 1690 252
Fragments arranged by size:
1690 252 209
BstYI r'GATC_y
Cuts at: 0 469 2151
Size: 469 1682
BstZ17I GTA'TAC
Cuts at: 0 1501 2151
Size: 1501 650
BtgI C'CryG_G
Cuts at: 0 17 2018 2151
Size: 17 2001 133
Fragments arranged by size:
2001 133 17
DraI TTT'AAA
Cuts at: 0 1194 2151
Size: 1194 957
EarI CTCTTCn'nnn_
Cuts at: 0 71 501 575 794 1043 1535 2151
Size: 71 430 74 219 249 492 616
Fragments arranged by size:
616 492 430 249 219 74 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1463 1781 2151
Size: 579 884 318 370
Fragments arranged by size:
884 579 370 318
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 1372 2151
Size: 277 1095 779
Fragments arranged by size:
1095 779 277
EcoO109I rG'GnC_Cy
Cuts at: 0 1891 1985 1986 2151
Size: 1891 94 1 165
Fragments arranged by size:
1891 165 94 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 606 638 2084 2116 2151
Size: 606 32 1446 32 35
Fragments arranged by size:
1446 606 35 32 32
MfeI C'AATT_G
Cuts at: 0 1103 2151
Size: 1103 1048
MslI CAynn'nnrTG
Cuts at: 0 1232 1370 2151
Size: 1232 138 781
Fragments arranged by size:
1232 781 138
MspA1I CmG'CkG
Cuts at: 0 31 499 1846 2151
Size: 31 468 1347 305
Fragments arranged by size:
1347 468 305 31
NdeI CA'TA_TG
Cuts at: 0 87 318 375 1035 2151
Size: 87 231 57 660 1116
Fragments arranged by size:
1116 660 231 87 57
NheI G'CTAG_C
Cuts at: 0 1659 1995 2151
Size: 1659 336 156
Fragments arranged by size:
1659 336 156
PpuMI rG'GwC_Cy
Cuts at: 0 1891 2151
Size: 1891 260
PsiI TTA'TAA
Cuts at: 0 814 1637 2151
Size: 814 823 514
Fragments arranged by size:
823 814 514
PspOMI G'GGCC_C
Cuts at: 0 1454 1985 2151
Size: 1454 531 166
Fragments arranged by size:
1454 531 166
PstI C_TGCA'G
Cuts at: 0 1245 1845 2151
Size: 1245 600 306
Fragments arranged by size:
1245 600 306
PvuII CAG'CTG
Cuts at: 0 31 499 1846 2151
Size: 31 468 1347 305
Fragments arranged by size:
1347 468 305 31
ScaI AGT'ACT
Cuts at: 0 2036 2151
Size: 2036 115
SexAI A'CCwGG_T
Cuts at: 0 1815 2151
Size: 1815 336
SfcI C'TryA_G
Cuts at: 0 137 464 1241 1263 1697 1841 2000 2151
Size: 137 327 777 22 434 144 159 151
Fragments arranged by size:
777 434 327 159 151 144 137 22
SmlI C'TyrA_G
Cuts at: 0 368 728 901 2151
Size: 368 360 173 1250
Fragments arranged by size:
1250 368 360 173
SpeI A'CTAG_T
Cuts at: 0 1569 2151
Size: 1569 582
SspI AAT'ATT
Cuts at: 0 594 1258 1482 2151
Size: 594 664 224 669
Fragments arranged by size:
669 664 594 224
StyI C'CwwG_G
Cuts at: 0 332 1124 2042 2151
Size: 332 792 918 109
Fragments arranged by size:
918 792 332 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 2151
Size: 417 1734
TatI w'GTAC_w
Cuts at: 0 209 1899 2034 2151
Size: 209 1690 135 117
Fragments arranged by size:
1690 209 135 117
XmnI GAAnn'nnTTC
Cuts at: 0 1073 2151
Size: 1073 1078
Enzymes that do cut:
AccI AflIII AlwNI ApaI ApoI AvaI BanI BanII
BbsI BclI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BsrGI BstYI BstZ17I
BtgI DraI EarI Eco57MI EcoNI EcoO109I Hin4I MfeI
MslI MspA1I NdeI NheI PpuMI PsiI PspOMI PstI
PvuII ScaI SexAI SfcI SmlI SpeI SspI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII Bpu10I BsaI BsaAI BsaHI BsgI BsiEI
BsiWI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI