Restriction map for
pF1KB5702
(Linear) (Six-base) MAPSORT of: KIBB5702.seq Check: 5833 from: 1 to: 2718
KIBB5702.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 440 1137 1700 2718
Size: 440 697 563 1018
Fragments arranged by size:
1018 697 563 440
AclI AA'CG_TT
Cuts at: 0 650 2718
Size: 650 2068
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 870 2120 2718
Size: 566 304 1250 598
Fragments arranged by size:
1250 598 566 304
AfeI AGC'GCT
Cuts at: 0 898 2718
Size: 898 1820
AflIII A'CryG_T
Cuts at: 0 973 2718
Size: 973 1745
AleI CACnn'nnGTG
Cuts at: 0 812 2718
Size: 812 1906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2313 2718
Size: 2313 405
AlwNI CAG_nnn'CTG
Cuts at: 0 1029 1038 1501 2325 2718
Size: 1029 9 463 824 393
Fragments arranged by size:
1029 824 463 393 9
ApaI G_GGCC'C
Cuts at: 0 140 2718
Size: 140 2578
ApoI r'AATT_y
Cuts at: 0 210 2718
Size: 210 2508
AseI AT'TA_AT
Cuts at: 0 1394 2718
Size: 1394 1324
AvaI C'yCGr_G
Cuts at: 0 383 1649 2006 2189 2718
Size: 383 1266 357 183 529
Fragments arranged by size:
1266 529 383 357 183
BamHI G'GATC_C
Cuts at: 0 39 2257 2718
Size: 39 2218 461
Fragments arranged by size:
2218 461 39
BanI G'GyrC_C
Cuts at: 0 2586 2718
Size: 2586 132
BanII G_rGCy'C
Cuts at: 0 140 2718
Size: 140 2578
BbsI GAAGACnn'nnnn_
Cuts at: 0 1126 1982 2718
Size: 1126 856 736
Fragments arranged by size:
1126 856 736
BbvCI CC'TCA_GC
Cuts at: 0 1792 2249 2689 2718
Size: 1792 457 440 29
Fragments arranged by size:
1792 457 440 29
BclI T'GATC_A
Cuts at: 0 612 2718
Size: 612 2106
BglI GCCn_nnn'nGGC
Cuts at: 0 712 955 1306 2718
Size: 712 243 351 1412
Fragments arranged by size:
1412 712 351 243
BlpI GC'TnA_GC
Cuts at: 0 2021 2718
Size: 2021 697
Bme1580I G_kGCm'C
Cuts at: 0 140 2007 2718
Size: 140 1867 711
Fragments arranged by size:
1867 711 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 2718
Size: 409 2309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 2718
Size: 934 1784
Bpu10I CC'TnA_GC
Cuts at: 0 1792 2249 2689 2718
Size: 1792 457 440 29
Fragments arranged by size:
1792 457 440 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 900 2718
Size: 241 659 1818
Fragments arranged by size:
1818 659 241
BsaAI yAC'GTr
Cuts at: 0 1662 2670 2718
Size: 1662 1008 48
Fragments arranged by size:
1662 1008 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 2718
Size: 235 30 2453
Fragments arranged by size:
2453 235 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 833 836 839 907 931 1921 2718
Size: 277 556 3 3 68 24 990 797
Fragments arranged by size:
990 797 556 277 68 24 3 3
BseYI C'CCAG_C
Cuts at: 0 1808 2718
Size: 1808 910
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1385 2419 2509 2701 2718
Size: 954 431 1034 90 192 17
Fragments arranged by size:
1034 954 431 192 90 17
BsmI GAATG_Cn'
Cuts at: 0 501 2484 2718
Size: 501 1983 234
Fragments arranged by size:
1983 501 234
Bsp1286I G_dGCh'C
Cuts at: 0 140 2007 2718
Size: 140 1867 711
Fragments arranged by size:
1867 711 140
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 2333 2345 2718
Size: 1046 1287 12 373
Fragments arranged by size:
1287 1046 373 12
BsrDI GCAATG_nn'
Cuts at: 0 510 1065 1580 2133 2285 2295 2718
Size: 510 555 515 553 152 10 423
Fragments arranged by size:
555 553 515 510 423 152 10
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1038 2325 2718
Size: 1038 1287 393
Fragments arranged by size:
1287 1038 393
BstBI TT'CG_AA
Cuts at: 0 291 1350 2718
Size: 291 1059 1368
Fragments arranged by size:
1368 1059 291
BstYI r'GATC_y
Cuts at: 0 39 1452 2257 2718
Size: 39 1413 805 461
Fragments arranged by size:
1413 805 461 39
Bsu36I CC'TnA_GG
Cuts at: 0 1385 1904 2718
Size: 1385 519 814
Fragments arranged by size:
1385 814 519
BtgI C'CryG_G
Cuts at: 0 1781 1814 2718
Size: 1781 33 904
Fragments arranged by size:
1781 904 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2331 2718
Size: 2331 387
BtsI GCAGTG_nn'
Cuts at: 0 782 1889 2718
Size: 782 1107 829
Fragments arranged by size:
1107 829 782
ClaI AT'CG_AT
Cuts at: 0 1835 2718
Size: 1835 883
DraI TTT'AAA
Cuts at: 0 1461 2718
Size: 1461 1257
EaeI y'GGCC_r
Cuts at: 0 603 956 1995 2063 2144 2277 2718
Size: 603 353 1039 68 81 133 441
Fragments arranged by size:
1039 603 441 353 133 81 68
EarI CTCTTCn'nnn_
Cuts at: 0 145 354 2605 2718
Size: 145 209 2251 113
Fragments arranged by size:
2251 209 145 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1009 2718
Size: 1009 1709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 870 934 2120 2718
Size: 566 304 64 1186 598
Fragments arranged by size:
1186 598 566 304 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2455 2718
Size: 2455 263
EcoO109I rG'GnC_Cy
Cuts at: 0 136 137 723 1026 1035 1898 2322 2685
Size: 136 1 586 303 9 863 424 363
Cuts at: 2685 2718
Size: 33
Fragments arranged by size:
863 586 424 363 303 136 33 9
1
HaeII r_GCGC'y
Cuts at: 0 900 2444 2718
Size: 900 1544 274
Fragments arranged by size:
1544 900 274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 331 363 784 816 2004 2036
Size: 48 32 251 32 421 32 1188 32
Cuts at: 2036 2242 2274 2718
Size: 206 32 444
Fragments arranged by size:
1188 444 421 251 206 48 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1342 2272 2718
Size: 1342 930 446
Fragments arranged by size:
1342 930 446
MluI A'CGCG_T
Cuts at: 0 973 2718
Size: 973 1745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1763 1892 2718
Size: 65 1698 129 826
Fragments arranged by size:
1698 826 129 65
MscI TGG'CCA
Cuts at: 0 605 1997 2065 2146 2718
Size: 605 1392 68 81 572
Fragments arranged by size:
1392 605 572 81 68
MslI CAynn'nnrTG
Cuts at: 0 695 812 1747 2137 2164 2718
Size: 695 117 935 390 27 554
Fragments arranged by size:
935 695 554 390 117 27
MspA1I CmG'CkG
Cuts at: 0 459 741 1624 1986 2367 2718
Size: 459 282 883 362 381 351
Fragments arranged by size:
883 459 381 362 351 282
NcoI C'CATG_G
Cuts at: 0 1814 2718
Size: 1814 904
NdeI CA'TA_TG
Cuts at: 0 837 2718
Size: 837 1881
NspI r_CATG'y
Cuts at: 0 683 1279 2718
Size: 683 596 1439
Fragments arranged by size:
1439 683 596
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1815 2718
Size: 180 1635 903
Fragments arranged by size:
1635 903 180
PfoI T'CCnGG_A
Cuts at: 0 1181 1865 2072 2260 2718
Size: 1181 684 207 188 458
Fragments arranged by size:
1181 684 458 207 188
PmlI CAC'GTG
Cuts at: 0 2670 2718
Size: 2670 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 2718
Size: 144 32 2542
Fragments arranged by size:
2542 144 32
PpuMI rG'GwC_Cy
Cuts at: 0 723 1035 1898 2322 2718
Size: 723 312 863 424 396
Fragments arranged by size:
863 723 424 396 312
PshAI GACnn'nnGTC
Cuts at: 0 7 1963 2718
Size: 7 1956 755
Fragments arranged by size:
1956 755 7
PspOMI G'GGCC_C
Cuts at: 0 136 2718
Size: 136 2582
PspXI vC'TCGA_Gb
Cuts at: 0 383 2718
Size: 383 2335
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 272 304 1331 1363 2718
Size: 272 32 1027 32 1355
Fragments arranged by size:
1355 1027 272 32 32
PstI C_TGCA'G
Cuts at: 0 324 627 644 746 1034 2380 2718
Size: 324 303 17 102 288 1346 338
Fragments arranged by size:
1346 338 324 303 288 102 17
PvuII CAG'CTG
Cuts at: 0 459 741 1624 1986 2367 2718
Size: 459 282 883 362 381 351
Fragments arranged by size:
883 459 381 362 351 282
SapI GCTCTTCn'nnn_
Cuts at: 0 354 2718
Size: 354 2364
SbfI CC_TGCA'GG
Cuts at: 0 1034 2718
Size: 1034 1684
SfcI C'TryA_G
Cuts at: 0 278 320 623 640 685 742 1030 2376
Size: 278 42 303 17 45 57 288 1346
Cuts at: 2376 2718
Size: 342
Fragments arranged by size:
1346 342 303 288 278 57 45 42
17
SmaI CCC'GGG
Cuts at: 0 1651 2008 2718
Size: 1651 357 710
Fragments arranged by size:
1651 710 357
SmlI C'TyrA_G
Cuts at: 0 256 383 879 2718
Size: 256 127 496 1839
Fragments arranged by size:
1839 496 256 127
SphI G_CATG'C
Cuts at: 0 683 2718
Size: 683 2035
SrfI GCCC'GGGC
Cuts at: 0 2008 2718
Size: 2008 710
StuI AGG'CCT
Cuts at: 0 906 2718
Size: 906 1812
StyI C'CwwG_G
Cuts at: 0 1814 2718
Size: 1814 904
TatI w'GTAC_w
Cuts at: 0 1049 2718
Size: 1049 1669
XbaI T'CTAG_A
Cuts at: 0 51 2718
Size: 51 2667
XhoI C'TCGA_G
Cuts at: 0 383 2718
Size: 383 2335
XmaI C'CCGG_G
Cuts at: 0 1649 2006 2718
Size: 1649 357 712
Fragments arranged by size:
1649 712 357
XmnI GAAnn'nnTTC
Cuts at: 0 287 2718
Size: 287 2431
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BclI BglI BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaAI BsaXI BseRI BseYI BsgI BsmI Bsp1286I
BspMI BsrDI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII MluI MmeI MscI MslI
MspA1I NcoI NdeI NspI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SapI
SbfI SfcI SmaI SmlI SphI SrfI StuI StyI
TatI XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AloI ApaLI
AscI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI
BglII BmgBI BmrI BmtI BsaI BsaBI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstZ17I CspCI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PsiI PvuI RsrII SacI SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI TaqII TstI Tth111I XcmI ZraI