(Linear) (Six-base) MAPSORT of: KIBB5700.seq  Check: 7670  from: 1  to: 1278



KIBB5700.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1061    1278 
   Size:       1061     217
 
ApoI r'AATT_y

Cuts at:      0     151     686     886    1278 
   Size:        151     535     200     392

  Fragments arranged by size: 

                 535     392     200     151
 
AvrII C'CTAG_G

Cuts at:      0     726    1278 
   Size:        726     552
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      80    1278 
   Size:         80    1198
 
BclI T'GATC_A

Cuts at:      0     863    1278 
   Size:        863     415
 
BglII A'GATC_T

Cuts at:      0     699    1278 
   Size:        699     579
 
BmgBI CAC'GTC

Cuts at:      0     172    1278 
   Size:        172    1106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     617    1133    1244    1278 
   Size:        400     217     516     111      34

  Fragments arranged by size: 

                 516     400     217     111      34
 
Bpu10I CC'TnA_GC

Cuts at:      0      20    1278 
   Size:         20    1258
 
BsaBI GATnn'nnATC

Cuts at:      0    1211    1278 
   Size:       1211      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311    1278 
   Size:        311     967
 
BsmI GAATG_Cn'

Cuts at:      0     316    1278 
   Size:        316     962
 
BsrGI T'GTAC_A

Cuts at:      0    1007    1278 
   Size:       1007     271
 
BstYI r'GATC_y

Cuts at:      0     294     699    1278 
   Size:        294     405     579

  Fragments arranged by size: 

                 579     405     294
 
DraIII CAC_nnn'GTG

Cuts at:      0     187    1278 
   Size:        187    1091
 
EarI CTCTTCn'nnn_

Cuts at:      0     665    1145    1278 
   Size:        665     480     133

  Fragments arranged by size: 

                 665     480     133
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58     520    1278 
   Size:         58     462     758

  Fragments arranged by size: 

                 758     462      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     617    1061    1133    1244    1278 
   Size:        400     217     444      72     111      34

  Fragments arranged by size: 

                 444     400     217     111      72      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1124    1278 
   Size:       1124     154
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     286     318     855     887    1278 
   Size:        286      32     537      32     391

  Fragments arranged by size: 

                 537     391     286      32      32
 
HincII GTy'rAC

Cuts at:      0     160    1278 
   Size:        160    1118
 
HindIII A'AGCT_T

Cuts at:      0    1106    1278 
   Size:       1106     172
 
NspI r_CATG'y

Cuts at:      0     618    1278 
   Size:        618     660
 
PasI CC'CwG_GG

Cuts at:      0     945    1278 
   Size:        945     333
 
PfoI T'CCnGG_A

Cuts at:      0     548     636    1278 
   Size:        548      88     642

  Fragments arranged by size: 

                 642     548      88
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     702     734    1278 
   Size:        702      32     544

  Fragments arranged by size: 

                 702     544      32
 
SfcI C'TryA_G

Cuts at:      0     260     305    1043    1278 
   Size:        260      45     738     235

  Fragments arranged by size: 

                 738     260     235      45
 
SpeI A'CTAG_T

Cuts at:      0     858    1278 
   Size:        858     420
 
StyI C'CwwG_G

Cuts at:      0     726    1278 
   Size:        726     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     958    1278 
   Size:        958     320
 
TatI w'GTAC_w

Cuts at:      0     337    1007    1278 
   Size:        337     670     271

  Fragments arranged by size: 

                 670     337     271
 
XbaI T'CTAG_A

Cuts at:      0     753    1278 
   Size:        753     525
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     952    1278 
   Size:        952     326
 
XmnI GAAnn'nnTTC

Cuts at:      0     761    1278 
   Size:        761     517
 

 Enzymes that do cut:

     AcuI     ApoI    AvrII     BbsI     BclI    BglII    BmgBI     BpmI
   Bpu10I    BsaBI     BsgI     BsmI    BsrGI    BstYI   DraIII     EarI
     EciI  Eco57MI     FalI    Hin4I   HincII  HindIII     NspI     PasI
     PfoI     PpiI     SfcI     SpeI     StyI    TaqII     TatI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XhoI
     XmaI     ZraI