Restriction map for
pF1KB5695
(Linear) (Six-base) MAPSORT of: KIBB5695.seq Check: 7137 from: 1 to: 1908
KIBB5695.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1908
Size: 1199 709
AflII C'TTAA_G
Cuts at: 0 619 651 826 1908
Size: 619 32 175 1082
Fragments arranged by size:
1082 619 175 32
AflIII A'CryG_T
Cuts at: 0 161 1908
Size: 161 1747
AgeI A'CCGG_T
Cuts at: 0 1891 1908
Size: 1891 17
AhdI GACnn_n'nnGTC
Cuts at: 0 1469 1908
Size: 1469 439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 743 1619 1908
Size: 743 876 289
Fragments arranged by size:
876 743 289
AlwNI CAG_nnn'CTG
Cuts at: 0 1495 1908
Size: 1495 413
ApaI G_GGCC'C
Cuts at: 0 107 1908
Size: 107 1801
ApoI r'AATT_y
Cuts at: 0 216 565 588 779 835 1908
Size: 216 349 23 191 56 1073
Fragments arranged by size:
1073 349 216 191 56 23
AvaI C'yCGr_G
Cuts at: 0 1789 1908
Size: 1789 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 861 894 1908
Size: 861 33 1014
Fragments arranged by size:
1014 861 33
BanI G'GyrC_C
Cuts at: 0 1321 1908
Size: 1321 587
BanII G_rGCy'C
Cuts at: 0 107 767 798 1908
Size: 107 660 31 1110
Fragments arranged by size:
1110 660 107 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1908
Size: 605 1303
BbvCI CC'TCA_GC
Cuts at: 0 1559 1908
Size: 1559 349
BclI T'GATC_A
Cuts at: 0 137 1908
Size: 137 1771
BlpI GC'TnA_GC
Cuts at: 0 1103 1908
Size: 1103 805
Bme1580I G_kGCm'C
Cuts at: 0 15 107 1908
Size: 15 92 1801
Fragments arranged by size:
1801 92 15
BmgBI CAC'GTC
Cuts at: 0 63 1477 1908
Size: 63 1414 431
Fragments arranged by size:
1414 431 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 2 1908
Size: 2 1906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1781 1908
Size: 1781 127
Bpu10I CC'TnA_GC
Cuts at: 0 1316 1559 1908
Size: 1316 243 349
Fragments arranged by size:
1316 349 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1649 1908
Size: 1649 259
BsaI GGTCTCn'nnnn_
Cuts at: 0 263 1392 1908
Size: 263 1129 516
Fragments arranged by size:
1129 516 263
BsaAI yAC'GTr
Cuts at: 0 42 1908
Size: 42 1866
BsaWI w'CCGG_w
Cuts at: 0 929 1891 1908
Size: 929 962 17
Fragments arranged by size:
962 929 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1011 1652 1908
Size: 1011 641 256
Fragments arranged by size:
1011 641 256
BseYI C'CCAG_C
Cuts at: 0 16 1365 1908
Size: 16 1349 543
Fragments arranged by size:
1349 543 16
BsiEI CG_ry'CG
Cuts at: 0 101 1908
Size: 101 1807
BsiHKAI G_wGCw'C
Cuts at: 0 767 798 1908
Size: 767 31 1110
Fragments arranged by size:
1110 767 31
BsmI GAATG_Cn'
Cuts at: 0 485 1908
Size: 485 1423
BsmBI CGTCTCn'nnnn_
Cuts at: 0 430 931 1908
Size: 430 501 977
Fragments arranged by size:
977 501 430
Bsp1286I G_dGCh'C
Cuts at: 0 15 107 767 798 1908
Size: 15 92 660 31 1110
Fragments arranged by size:
1110 660 92 31 15
BspEI T'CCGG_A
Cuts at: 0 929 1908
Size: 929 979
BsrBI CCG'CTC
Cuts at: 0 149 1908
Size: 149 1759
BsrFI r'CCGG_y
Cuts at: 0 183 1891 1908
Size: 183 1708 17
Fragments arranged by size:
1708 183 17
BsrGI T'GTAC_A
Cuts at: 0 426 1614 1908
Size: 426 1188 294
Fragments arranged by size:
1188 426 294
BssSI C'ACGA_G
Cuts at: 0 1421 1908
Size: 1421 487
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1495 1908
Size: 1495 413
BstYI r'GATC_y
Cuts at: 0 624 1908
Size: 624 1284
BtgI C'CryG_G
Cuts at: 0 26 1908
Size: 26 1882
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 88 1908
Size: 88 1820
DraIII CAC_nnn'GTG
Cuts at: 0 1441 1908
Size: 1441 467
EaeI y'GGCC_r
Cuts at: 0 98 1073 1908
Size: 98 975 835
Fragments arranged by size:
975 835 98
EagI C'GGCC_G
Cuts at: 0 98 1908
Size: 98 1810
EarI CTCTTCn'nnn_
Cuts at: 0 1109 1908
Size: 1109 799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 1351 1908
Size: 202 1149 557
Fragments arranged by size:
1149 557 202
EcoICRI GAG'CTC
Cuts at: 0 765 796 1908
Size: 765 31 1112
Fragments arranged by size:
1112 765 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1781 1908
Size: 1199 582 127
Fragments arranged by size:
1199 582 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1214 1908
Size: 1214 694
EcoO109I rG'GnC_Cy
Cuts at: 0 48 1908
Size: 48 1860
EcoRI G'AATT_C
Cuts at: 0 565 1908
Size: 565 1343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 562 645 1908
Size: 562 83 1263
Fragments arranged by size:
1263 562 83
HaeII r_GCGC'y
Cuts at: 0 618 1122 1908
Size: 618 504 786
Fragments arranged by size:
786 618 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1455 1487 1908
Size: 1455 32 421
Fragments arranged by size:
1455 421 32
HincII GTy'rAC
Cuts at: 0 1433 1881 1908
Size: 1433 448 27
Fragments arranged by size:
1433 448 27
HindIII A'AGCT_T
Cuts at: 0 708 1908
Size: 708 1200
HpaI GTT'AAC
Cuts at: 0 1881 1908
Size: 1881 27
MluI A'CGCG_T
Cuts at: 0 161 1908
Size: 161 1747
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1736 1908
Size: 489 1247 172
Fragments arranged by size:
1247 489 172
MscI TGG'CCA
Cuts at: 0 1075 1908
Size: 1075 833
MslI CAynn'nnrTG
Cuts at: 0 725 1571 1747 1908
Size: 725 846 176 161
Fragments arranged by size:
846 725 176 161
MspA1I CmG'CkG
Cuts at: 0 451 1369 1486 1495 1908
Size: 451 918 117 9 413
Fragments arranged by size:
918 451 413 117 9
NaeI GCC'GGC
Cuts at: 0 185 1908
Size: 185 1723
NcoI C'CATG_G
Cuts at: 0 26 1908
Size: 26 1882
NdeI CA'TA_TG
Cuts at: 0 1784 1908
Size: 1784 124
NgoMIV G'CCGG_C
Cuts at: 0 183 1908
Size: 183 1725
NspI r_CATG'y
Cuts at: 0 1752 1908
Size: 1752 156
PpuMI rG'GwC_Cy
Cuts at: 0 48 1908
Size: 48 1860
PsiI TTA'TAA
Cuts at: 0 229 1908
Size: 229 1679
PspOMI G'GGCC_C
Cuts at: 0 103 1908
Size: 103 1805
PspXI vC'TCGA_Gb
Cuts at: 0 1789 1908
Size: 1789 119
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1157 1189 1908
Size: 1157 32 719
Fragments arranged by size:
1157 719 32
PstI C_TGCA'G
Cuts at: 0 130 1485 1491 1500 1545 1908
Size: 130 1355 6 9 45 363
Fragments arranged by size:
1355 363 130 45 9 6
PvuII CAG'CTG
Cuts at: 0 451 1369 1486 1908
Size: 451 918 117 422
Fragments arranged by size:
918 451 422 117
SacI G_AGCT'C
Cuts at: 0 767 798 1908
Size: 767 31 1110
Fragments arranged by size:
1110 767 31
SapI GCTCTTCn'nnn_
Cuts at: 0 1109 1908
Size: 1109 799
ScaI AGT'ACT
Cuts at: 0 1392 1908
Size: 1392 516
SfcI C'TryA_G
Cuts at: 0 126 1481 1487 1496 1541 1829 1908
Size: 126 1355 6 9 45 288 79
Fragments arranged by size:
1355 288 126 79 45 9 6
SgrAI Cr'CCGG_yG
Cuts at: 0 1891 1908
Size: 1891 17
SmlI C'TyrA_G
Cuts at: 0 619 651 826 1664 1789 1908
Size: 619 32 175 838 125 119
Fragments arranged by size:
838 619 175 125 119 32
SpeI A'CTAG_T
Cuts at: 0 961 1908
Size: 961 947
StyI C'CwwG_G
Cuts at: 0 26 152 410 1908
Size: 26 126 258 1498
Fragments arranged by size:
1498 258 126 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 1675 1908
Size: 82 1593 233
Fragments arranged by size:
1593 233 82
TatI w'GTAC_w
Cuts at: 0 426 1390 1614 1836 1908
Size: 426 964 224 222 72
Fragments arranged by size:
964 426 224 222 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1881 1908
Size: 1881 27
Tth111I GACn'n_nGTC
Cuts at: 0 1298 1908
Size: 1298 610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 1304 1908
Size: 24 1280 604
Fragments arranged by size:
1280 604 24
XhoI C'TCGA_G
Cuts at: 0 1789 1908
Size: 1789 119
XmnI GAAnn'nnTTC
Cuts at: 0 1778 1908
Size: 1778 130
Enzymes that do cut:
AcuI AflII AflIII AgeI AhdI AlfI AlwNI ApaI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BclI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrFI BsrGI BssSI BstAPI BstYI
BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuII SacI SapI ScaI SfcI SgrAI
SmlI SpeI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AleI AloI
ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI
BciVI BfrBI BglI BglII BmtI BplI BsaBI BsaHI
BsaXI BsgI BsiWI BspHI BspMI BsrDI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EcoRV FseI FspI FspAI KasI KpnI MfeI
NarI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SmaI SnaBI SphI SrfI SspI StuI SwaI XbaI
XmaI ZraI