Restriction map for
pF1KB5694
(Linear) (Six-base) MAPSORT of: KIBB5694.seq Check: 2532 from: 1 to: 1938
KIBB5694.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 1037 1938
Size: 1037 901
AccI GT'mk_AC
Cuts at: 0 179 395 1938
Size: 179 216 1543
Fragments arranged by size:
1543 216 179
Acc65I G'GTAC_C
Cuts at: 0 497 1938
Size: 497 1441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 562 643 805 828 1159 1938
Size: 157 405 81 162 23 331 779
Fragments arranged by size:
779 405 331 162 157 81 23
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1938
Size: 1105 32 801
Fragments arranged by size:
1105 801 32
AlwNI CAG_nnn'CTG
Cuts at: 0 966 1057 1131 1155 1938
Size: 966 91 74 24 783
Fragments arranged by size:
966 783 91 74 24
AvaI C'yCGr_G
Cuts at: 0 1117 1938
Size: 1117 821
BanI G'GyrC_C
Cuts at: 0 497 1367 1938
Size: 497 870 571
Fragments arranged by size:
870 571 497
BanII G_rGCy'C
Cuts at: 0 674 773 1899 1938
Size: 674 99 1126 39
Fragments arranged by size:
1126 674 99 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 650 1938
Size: 650 1288
BbvCI CC'TCA_GC
Cuts at: 0 169 1594 1938
Size: 169 1425 344
Fragments arranged by size:
1425 344 169
BglI GCCn_nnn'nGGC
Cuts at: 0 1770 1938
Size: 1770 168
BlpI GC'TnA_GC
Cuts at: 0 83 274 1938
Size: 83 191 1664
Fragments arranged by size:
1664 191 83
Bme1580I G_kGCm'C
Cuts at: 0 74 1235 1370 1938
Size: 74 1161 135 568
Fragments arranged by size:
1161 568 135 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 651 683 1938
Size: 147 504 32 1255
Fragments arranged by size:
1255 504 147 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 940 1561 1938
Size: 265 675 621 377
Fragments arranged by size:
675 621 377 265
Bpu10I CC'TnA_GC
Cuts at: 0 98 169 1441 1594 1776 1938
Size: 98 71 1272 153 182 162
Fragments arranged by size:
1272 182 162 153 98 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 494 1938
Size: 296 198 1444
Fragments arranged by size:
1444 296 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 1898 1938
Size: 1898 40
BsaHI Gr'CG_yC
Cuts at: 0 1034 1225 1938
Size: 1034 191 713
Fragments arranged by size:
1034 713 191
BsaWI w'CCGG_w
Cuts at: 0 405 1938
Size: 405 1533
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1621 1651 1938
Size: 1621 30 287
Fragments arranged by size:
1621 287 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 481 562 1938
Size: 424 57 81 1376
Fragments arranged by size:
1376 424 81 57
BseYI C'CCAG_C
Cuts at: 0 865 1234 1722 1938
Size: 865 369 488 216
Fragments arranged by size:
865 488 369 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1788 1938
Size: 1788 150
BsiHKAI G_wGCw'C
Cuts at: 0 674 1447 1938
Size: 674 773 491
Fragments arranged by size:
773 674 491
BsmI GAATG_Cn'
Cuts at: 0 785 1300 1498 1938
Size: 785 515 198 440
Fragments arranged by size:
785 515 440 198
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1811 1938
Size: 1811 127
Bsp1286I G_dGCh'C
Cuts at: 0 74 674 773 1235 1370 1447 1899 1938
Size: 74 600 99 462 135 77 452 39
Fragments arranged by size:
600 462 452 135 99 77 74 39
BspEI T'CCGG_A
Cuts at: 0 405 1938
Size: 405 1533
BsrFI r'CCGG_y
Cuts at: 0 533 680 1938
Size: 533 147 1258
Fragments arranged by size:
1258 533 147
BsrGI T'GTAC_A
Cuts at: 0 602 1938
Size: 602 1336
BssSI C'ACGA_G
Cuts at: 0 1459 1938
Size: 1459 479
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1512 1938
Size: 1512 426
BstYI r'GATC_y
Cuts at: 0 1425 1938
Size: 1425 513
Bsu36I CC'TnA_GG
Cuts at: 0 464 1938
Size: 464 1474
BtgI C'CryG_G
Cuts at: 0 773 1938
Size: 773 1165
BtsI GCAGTG_nn'
Cuts at: 0 1604 1938
Size: 1604 334
ClaI AT'CG_AT
Cuts at: 0 1925 1938
Size: 1925 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 588 907 1938
Size: 588 319 1031
Fragments arranged by size:
1031 588 319
EaeI y'GGCC_r
Cuts at: 0 531 1274 1938
Size: 531 743 664
Fragments arranged by size:
743 664 531
EarI CTCTTCn'nnn_
Cuts at: 0 881 1133 1540 1850 1938
Size: 881 252 407 310 88
Fragments arranged by size:
881 407 310 252 88
EcoICRI GAG'CTC
Cuts at: 0 672 1938
Size: 672 1266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 265 562 643 805 828 940 1159
Size: 157 108 297 81 162 23 112 219
Cuts at: 1159 1561 1938
Size: 402 377
Fragments arranged by size:
402 377 297 219 162 157 112 108
81 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1870 1938
Size: 1870 68
EcoO109I rG'GnC_Cy
Cuts at: 0 909 963 1113 1165 1938
Size: 909 54 150 52 773
Fragments arranged by size:
909 773 150 54 52
EcoRV GAT'ATC
Cuts at: 0 986 1938
Size: 986 952
FseI GG_CCGG'CC
Cuts at: 0 537 1938
Size: 537 1401
HaeII r_GCGC'y
Cuts at: 0 360 951 1730 1776 1938
Size: 360 591 779 46 162
Fragments arranged by size:
779 591 360 162 46
HincII GTy'rAC
Cuts at: 0 180 429 1269 1398 1938
Size: 180 249 840 129 540
Fragments arranged by size:
840 540 249 180 129
KpnI G_GTAC'C
Cuts at: 0 501 1938
Size: 501 1437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1457 1529 1601 1938
Size: 1457 72 72 337
Fragments arranged by size:
1457 337 72 72
MscI TGG'CCA
Cuts at: 0 1276 1938
Size: 1276 662
MslI CAynn'nnrTG
Cuts at: 0 445 1938
Size: 445 1493
MspA1I CmG'CkG
Cuts at: 0 270 633 1263 1883 1938
Size: 270 363 630 620 55
Fragments arranged by size:
630 620 363 270 55
NaeI GCC'GGC
Cuts at: 0 535 1938
Size: 535 1403
NgoMIV G'CCGG_C
Cuts at: 0 533 1938
Size: 533 1405
PasI CC'CwG_GG
Cuts at: 0 435 1938
Size: 435 1503
PflMI CCAn_nnn'nTGG
Cuts at: 0 585 1155 1388 1938
Size: 585 570 233 550
Fragments arranged by size:
585 570 550 233
PfoI T'CCnGG_A
Cuts at: 0 281 1387 1538 1899 1938
Size: 281 1106 151 361 39
Fragments arranged by size:
1106 361 281 151 39
PpuMI rG'GwC_Cy
Cuts at: 0 909 1113 1938
Size: 909 204 825
Fragments arranged by size:
909 825 204
PshAI GACnn'nnGTC
Cuts at: 0 313 1938
Size: 313 1625
PspXI vC'TCGA_Gb
Cuts at: 0 1117 1938
Size: 1117 821
PstI C_TGCA'G
Cuts at: 0 1874 1938
Size: 1874 64
PvuII CAG'CTG
Cuts at: 0 633 1263 1938
Size: 633 630 675
Fragments arranged by size:
675 633 630
SacI G_AGCT'C
Cuts at: 0 674 1938
Size: 674 1264
SalI G'TCGA_C
Cuts at: 0 178 1938
Size: 178 1760
SbfI CC_TGCA'GG
Cuts at: 0 1874 1938
Size: 1874 64
SfcI C'TryA_G
Cuts at: 0 1870 1938
Size: 1870 68
SmlI C'TyrA_G
Cuts at: 0 311 509 1117 1938
Size: 311 198 608 821
Fragments arranged by size:
821 608 311 198
StyI C'CwwG_G
Cuts at: 0 159 1291 1938
Size: 159 1132 647
Fragments arranged by size:
1132 647 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 353 1311 1938
Size: 353 958 627
Fragments arranged by size:
958 627 353
TatI w'GTAC_w
Cuts at: 0 602 1938
Size: 602 1336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 796 828 1421 1453 1596 1628 1938
Size: 796 32 593 32 143 32 310
Fragments arranged by size:
796 593 310 143 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 250 1938
Size: 250 1688
XhoI C'TCGA_G
Cuts at: 0 1117 1938
Size: 1117 821
ZraI GAC'GTC
Cuts at: 0 1035 1938
Size: 1035 903
Enzymes that do cut:
AatII AccI Acc65I AcuI AloI AlwNI AvaI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrFI BsrGI
BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FseI
HaeII HincII KpnI MmeI MscI MslI MspA1I NaeI
NgoMIV PasI PflMI PfoI PpuMI PshAI PspXI PstI
PvuII SacI SalI SbfI SfcI SmlI StyI TaqII
TatI TstI XcmI XhoI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BsaAI BsaBI BsiEI BsiWI BspHI
BspMI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI CspCI DraI DraIII EagI EciI EcoRI FalI
FspI FspAI Hin4I HindIII HpaI KasI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PsiI PspOMI PsrI
PvuI RsrII SacII SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XmaI XmnI