Restriction map for
pF1KB5680
(Linear) (Six-base) MAPSORT of: KIBB5680.seq Check: 356 from: 1 to: 1440
KIBB5680.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 478 658 1440
Size: 413 65 180 782
Fragments arranged by size:
782 413 180 65
AflIII A'CryG_T
Cuts at: 0 1244 1440
Size: 1244 196
AhdI GACnn_n'nnGTC
Cuts at: 0 360 1440
Size: 360 1080
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 245 277 715 747 1440
Size: 245 32 438 32 693
Fragments arranged by size:
693 438 245 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 391 1440
Size: 391 1049
ApaI G_GGCC'C
Cuts at: 0 750 1440
Size: 750 690
AvaI C'yCGr_G
Cuts at: 0 719 952 1120 1440
Size: 719 233 168 320
Fragments arranged by size:
719 320 233 168
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 925 958 1440
Size: 925 33 482
Fragments arranged by size:
925 482 33
BanI G'GyrC_C
Cuts at: 0 97 617 907 1440
Size: 97 520 290 533
Fragments arranged by size:
533 520 290 97
BanII G_rGCy'C
Cuts at: 0 370 750 1079 1186 1440
Size: 370 380 329 107 254
Fragments arranged by size:
380 370 329 254 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 925 1440
Size: 196 729 515
Fragments arranged by size:
729 515 196
BclI T'GATC_A
Cuts at: 0 1337 1440
Size: 1337 103
BlpI GC'TnA_GC
Cuts at: 0 1261 1440
Size: 1261 179
Bme1580I G_kGCm'C
Cuts at: 0 478 750 860 1440
Size: 478 272 110 580
Fragments arranged by size:
580 478 272 110
BmgBI CAC'GTC
Cuts at: 0 9 1289 1440
Size: 9 1280 151
Fragments arranged by size:
1280 151 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 1404 1440
Size: 368 1036 36
Fragments arranged by size:
1036 368 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 850 982 1440
Size: 597 253 132 458
Fragments arranged by size:
597 458 253 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 313 526 808 1135 1440
Size: 70 243 213 282 327 305
Fragments arranged by size:
327 305 282 243 213 70
BsaAI yAC'GTr
Cuts at: 0 1245 1440
Size: 1245 195
BsaBI GATnn'nnATC
Cuts at: 0 1342 1440
Size: 1342 98
BsaHI Gr'CG_yC
Cuts at: 0 1193 1440
Size: 1193 247
BsaWI w'CCGG_w
Cuts at: 0 359 1440
Size: 359 1081
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 1440
Size: 715 30 695
Fragments arranged by size:
715 695 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 295 304 355 358 361 1105 1333
Size: 73 222 9 51 3 3 744 228
Cuts at: 1333 1440
Size: 107
Fragments arranged by size:
744 228 222 107 73 51 9 3
3
BseYI C'CCAG_C
Cuts at: 0 465 1002 1440
Size: 465 537 438
Fragments arranged by size:
537 465 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 775 1004 1440
Size: 19 756 229 436
Fragments arranged by size:
756 436 229 19
BsiEI CG_ry'CG
Cuts at: 0 124 364 663 981 1035 1440
Size: 124 240 299 318 54 405
Fragments arranged by size:
405 318 299 240 124 54
BsiHKAI G_wGCw'C
Cuts at: 0 1079 1440
Size: 1079 361
Bsp1286I G_dGCh'C
Cuts at: 0 370 478 750 860 1079 1186 1440
Size: 370 108 272 110 219 107 254
Fragments arranged by size:
370 272 254 219 110 108 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 797 1440
Size: 797 643
BsrBI CCG'CTC
Cuts at: 0 120 198 285 1440
Size: 120 78 87 1155
Fragments arranged by size:
1155 120 87 78
BsrFI r'CCGG_y
Cuts at: 0 1225 1440
Size: 1225 215
BstYI r'GATC_y
Cuts at: 0 536 1094 1440
Size: 536 558 346
Fragments arranged by size:
558 536 346
Bsu36I CC'TnA_GG
Cuts at: 0 278 1440
Size: 278 1162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1198 1440
Size: 1198 242
BtsI GCAGTG_nn'
Cuts at: 0 181 241 992 1440
Size: 181 60 751 448
Fragments arranged by size:
751 448 181 60
DraIII CAC_nnn'GTG
Cuts at: 0 376 432 1440
Size: 376 56 1008
Fragments arranged by size:
1008 376 56
EaeI y'GGCC_r
Cuts at: 0 67 121 413 560 978 1424 1440
Size: 67 54 292 147 418 446 16
Fragments arranged by size:
446 418 292 147 67 54 16
EagI C'GGCC_G
Cuts at: 0 121 978 1440
Size: 121 857 462
Fragments arranged by size:
857 462 121
EarI CTCTTCn'nnn_
Cuts at: 0 389 1440
Size: 389 1051
EcoICRI GAG'CTC
Cuts at: 0 1077 1440
Size: 1077 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 478 597 658 850 982 1440
Size: 413 65 119 61 192 132 458
Fragments arranged by size:
458 413 192 132 119 65 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 544 1087 1440
Size: 499 45 543 353
Fragments arranged by size:
543 499 353 45
EcoO109I rG'GnC_Cy
Cuts at: 0 819 1158 1397 1440
Size: 819 339 239 43
Fragments arranged by size:
819 339 239 43
HaeII r_GCGC'y
Cuts at: 0 764 1440
Size: 764 676
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 757 789 1017 1049 1114 1146
Size: 346 32 379 32 228 32 65 32
Cuts at: 1146 1440
Size: 294
Fragments arranged by size:
379 346 294 228 65 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1066 1440
Size: 1066 374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1440
Size: 1271 169
MscI TGG'CCA
Cuts at: 0 415 562 1440
Size: 415 147 878
Fragments arranged by size:
878 415 147
MslI CAynn'nnrTG
Cuts at: 0 1084 1440
Size: 1084 356
MspA1I CmG'CkG
Cuts at: 0 183 227 1430 1440
Size: 183 44 1203 10
Fragments arranged by size:
1203 183 44 10
NotI GC'GGCC_GC
Cuts at: 0 121 1440
Size: 121 1319
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 1440
Size: 315 1125
PmlI CAC'GTG
Cuts at: 0 1245 1440
Size: 1245 195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 1440
Size: 715 32 693
Fragments arranged by size:
715 693 32
PpuMI rG'GwC_Cy
Cuts at: 0 819 1158 1440
Size: 819 339 282
Fragments arranged by size:
819 339 282
PshAI GACnn'nnGTC
Cuts at: 0 244 1440
Size: 244 1196
PspOMI G'GGCC_C
Cuts at: 0 746 1440
Size: 746 694
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 796 828 1440
Size: 796 32 612
Fragments arranged by size:
796 612 32
PstI C_TGCA'G
Cuts at: 0 236 608 1440
Size: 236 372 832
Fragments arranged by size:
832 372 236
PvuII CAG'CTG
Cuts at: 0 183 1440
Size: 183 1257
SacI G_AGCT'C
Cuts at: 0 1079 1440
Size: 1079 361
ScaI AGT'ACT
Cuts at: 0 643 1440
Size: 643 797
SexAI A'CCwGG_T
Cuts at: 0 1303 1440
Size: 1303 137
SfcI C'TryA_G
Cuts at: 0 232 604 1440
Size: 232 372 836
Fragments arranged by size:
836 372 232
SmaI CCC'GGG
Cuts at: 0 721 1440
Size: 721 719
SmlI C'TyrA_G
Cuts at: 0 85 328 541 823 1150 1440
Size: 85 243 213 282 327 290
Fragments arranged by size:
327 290 282 243 213 85
StyI C'CwwG_G
Cuts at: 0 563 1196 1440
Size: 563 633 244
Fragments arranged by size:
633 563 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 387 1440
Size: 387 1053
TatI w'GTAC_w
Cuts at: 0 50 641 1440
Size: 50 591 799
Fragments arranged by size:
799 591 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 1101 1133 1440
Size: 62 32 1007 32 307
Fragments arranged by size:
1007 307 62 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 570 1440
Size: 312 258 870
Fragments arranged by size:
870 312 258
XmaI C'CCGG_G
Cuts at: 0 719 1440
Size: 719 721
Enzymes that do cut:
AcuI AflIII AhdI AloI AlwNI ApaI AvaI BaeI
BanI BanII BbsI BclI BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BstYI Bsu36I BtgZI BtsI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NotI PflMI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII SacI ScaI SexAI
SfcI SmaI SmlI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BmtI BplI Bpu10I BsaI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgI ClaI CspCI DraI DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmnI ZraI