Restriction map for
pF1KB5678
(Linear) (Six-base) MAPSORT of: KIBB5678.seq Check: 2262 from: 1 to: 2334
KIBB5678.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 2084 2334
Size: 1396 688 250
Fragments arranged by size:
1396 688 250
AfeI AGC'GCT
Cuts at: 0 1480 2334
Size: 1480 854
AflII C'TTAA_G
Cuts at: 0 1450 2334
Size: 1450 884
AleI CACnn'nnGTG
Cuts at: 0 1465 2334
Size: 1465 869
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1876 1908 2334
Size: 1876 32 426
Fragments arranged by size:
1876 426 32
AlwNI CAG_nnn'CTG
Cuts at: 0 208 711 1323 1384 2334
Size: 208 503 612 61 950
Fragments arranged by size:
950 612 503 208 61
ApaI G_GGCC'C
Cuts at: 0 469 804 2111 2334
Size: 469 335 1307 223
Fragments arranged by size:
1307 469 335 223
ApoI r'AATT_y
Cuts at: 0 1567 2186 2334
Size: 1567 619 148
Fragments arranged by size:
1567 619 148
AseI AT'TA_AT
Cuts at: 0 755 2334
Size: 755 1579
AvaI C'yCGr_G
Cuts at: 0 1394 1630 2057 2334
Size: 1394 236 427 277
Fragments arranged by size:
1394 427 277 236
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 633 666 2334
Size: 633 33 1668
Fragments arranged by size:
1668 633 33
BamHI G'GATC_C
Cuts at: 0 1283 2334
Size: 1283 1051
BanI G'GyrC_C
Cuts at: 0 1467 2308 2334
Size: 1467 841 26
Fragments arranged by size:
1467 841 26
BanII G_rGCy'C
Cuts at: 0 469 804 967 2111 2334
Size: 469 335 163 1144 223
Fragments arranged by size:
1144 469 335 223 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 68 2334
Size: 68 2266
BbvCI CC'TCA_GC
Cuts at: 0 47 2045 2334
Size: 47 1998 289
Fragments arranged by size:
1998 289 47
BglI GCCn_nnn'nGGC
Cuts at: 0 35 439 613 808 1735 2334
Size: 35 404 174 195 927 599
Fragments arranged by size:
927 599 404 195 174 35
BglII A'GATC_T
Cuts at: 0 104 1803 2334
Size: 104 1699 531
Fragments arranged by size:
1699 531 104
Bme1580I G_kGCm'C
Cuts at: 0 311 469 804 1472 1868 2111 2334
Size: 311 158 335 668 396 243 223
Fragments arranged by size:
668 396 335 311 243 223 158
BmgBI CAC'GTC
Cuts at: 0 958 2334
Size: 958 1376
BmrI ACTGGGnnnn_n'
Cuts at: 0 997 1015 1033 1051 1660 2334
Size: 997 18 18 18 609 674
Fragments arranged by size:
997 674 609 18 18 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 843 953 1169 2133 2214 2334
Size: 733 110 110 216 964 81 120
Fragments arranged by size:
964 733 216 120 110 110 81
Bpu10I CC'TnA_GC
Cuts at: 0 47 2045 2334
Size: 47 1998 289
Fragments arranged by size:
1998 289 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 631 1687 1964 2334
Size: 631 1056 277 370
Fragments arranged by size:
1056 631 370 277
BsaBI GATnn'nnATC
Cuts at: 0 1523 2334
Size: 1523 811
BsaHI Gr'CG_yC
Cuts at: 0 892 2334
Size: 892 1442
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1778 1808 1876 1906 2334
Size: 1778 30 68 30 428
Fragments arranged by size:
1778 428 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1675 2334
Size: 22 1653 659
Fragments arranged by size:
1653 659 22
BseYI C'CCAG_C
Cuts at: 0 2117 2334
Size: 2117 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 815 1370 1973 2334
Size: 659 156 555 603 361
Fragments arranged by size:
659 603 555 361 156
BsiHKAI G_wGCw'C
Cuts at: 0 884 2334
Size: 884 1450
BsmI GAATG_Cn'
Cuts at: 0 249 453 2226 2334
Size: 249 204 1773 108
Fragments arranged by size:
1773 249 204 108
Bsp1286I G_dGCh'C
Cuts at: 0 311 469 804 884 967 1472 1868 2111
Size: 311 158 335 80 83 505 396 243
Cuts at: 2111 2334
Size: 223
Fragments arranged by size:
505 396 335 311 243 223 158 83
80
BspHI T'CATG_A
Cuts at: 0 1712 2334
Size: 1712 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1331 1349 2334
Size: 1331 18 985
Fragments arranged by size:
1331 985 18
BsrBI CCG'CTC
Cuts at: 0 2228 2334
Size: 2228 106
BsrDI GCAATG_nn'
Cuts at: 0 1949 2334
Size: 1949 385
BsrFI r'CCGG_y
Cuts at: 0 2251 2334
Size: 2251 83
BssSI C'ACGA_G
Cuts at: 0 1976 2334
Size: 1976 358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1323 2334
Size: 1323 1011
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 513 902 992 1010 1028 1046 1064
Size: 183 330 389 90 18 18 18 18
Cuts at: 1064 1118 1136 1154 1172 1226 1244 1280 1313
Size: 54 18 18 18 54 18 36 33
Cuts at: 1313 2334
Size: 1021
Fragments arranged by size:
1021 389 330 183 90 54 54 36
33 18 18 18 18 18 18 18
18
BstYI r'GATC_y
Cuts at: 0 104 1283 1803 2172 2334
Size: 104 1179 520 369 162
Fragments arranged by size:
1179 520 369 162 104
Bsu36I CC'TnA_GG
Cuts at: 0 915 2334
Size: 915 1419
BtsI GCAGTG_nn'
Cuts at: 0 789 1231 2334
Size: 789 442 1103
Fragments arranged by size:
1103 789 442
DraI TTT'AAA
Cuts at: 0 234 2334
Size: 234 2100
DrdI GACnn_nn'nnGTC
Cuts at: 0 825 2334
Size: 825 1509
EaeI y'GGCC_r
Cuts at: 0 515 614 1030 1066 1102 1210 1228 1246
Size: 515 99 416 36 36 108 18 18
Cuts at: 1246 1264 1297 2334
Size: 18 33 1037
Fragments arranged by size:
1037 515 416 108 99 36 36 33
18 18 18
EarI CTCTTCn'nnn_
Cuts at: 0 767 2334
Size: 767 1567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 843 953 1169 1396 2084 2133 2214
Size: 733 110 110 216 227 688 49 81
Cuts at: 2214 2334
Size: 120
Fragments arranged by size:
733 688 227 216 120 110 110 81
49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1411 1747 2334
Size: 1411 336 587
Fragments arranged by size:
1411 587 336
EcoO109I rG'GnC_Cy
Cuts at: 0 567 743 801 818 1180 1198 1902 2108
Size: 567 176 58 17 362 18 704 206
Cuts at: 2108 2334
Size: 226
Fragments arranged by size:
704 567 362 226 206 176 58 18
17
EcoRI G'AATT_C
Cuts at: 0 2186 2334
Size: 2186 148
EcoRV GAT'ATC
Cuts at: 0 1500 2334
Size: 1500 834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 246 348 2334
Size: 246 102 1986
Fragments arranged by size:
1986 246 102
HaeII r_GCGC'y
Cuts at: 0 71 1482 2334
Size: 71 1411 852
Fragments arranged by size:
1411 852 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1487 1519 1778 1810 2086 2118 2164 2196
Size: 1487 32 259 32 276 32 46 32
Cuts at: 2196 2334
Size: 138
Fragments arranged by size:
1487 276 259 138 46 32 32 32
32
MscI TGG'CCA
Cuts at: 0 517 616 1032 1068 1230 1248 1266 1299
Size: 517 99 416 36 162 18 18 33
Cuts at: 1299 2334
Size: 1035
Fragments arranged by size:
1035 517 416 162 99 36 33 18
18
MslI CAynn'nnrTG
Cuts at: 0 1134 1152 1465 1711 2219 2334
Size: 1134 18 313 246 508 115
Fragments arranged by size:
1134 508 313 246 115 18
MspA1I CmG'CkG
Cuts at: 0 142 208 577 1291 2044 2334
Size: 142 66 369 714 753 290
Fragments arranged by size:
753 714 369 290 142 66
NspI r_CATG'y
Cuts at: 0 1550 2334
Size: 1550 784
PflMI CCAn_nnn'nTGG
Cuts at: 0 1082 1262 2334
Size: 1082 180 1072
Fragments arranged by size:
1082 1072 180
PfoI T'CCnGG_A
Cuts at: 0 718 2334
Size: 718 1616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1876 1908 2334
Size: 1876 32 426
Fragments arranged by size:
1876 426 32
PpuMI rG'GwC_Cy
Cuts at: 0 743 818 1180 1198 2334
Size: 743 75 362 18 1136
Fragments arranged by size:
1136 743 362 75 18
PspOMI G'GGCC_C
Cuts at: 0 465 800 2107 2334
Size: 465 335 1307 227
Fragments arranged by size:
1307 465 335 227
PstI C_TGCA'G
Cuts at: 0 816 2010 2043 2334
Size: 816 1194 33 291
Fragments arranged by size:
1194 816 291 33
PvuII CAG'CTG
Cuts at: 0 142 208 577 2044 2334
Size: 142 66 369 1467 290
Fragments arranged by size:
1467 369 290 142 66
ScaI AGT'ACT
Cuts at: 0 1441 2334
Size: 1441 893
SfcI C'TryA_G
Cuts at: 0 812 1854 2006 2039 2334
Size: 812 1042 152 33 295
Fragments arranged by size:
1042 812 295 152 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 439 613 2334
Size: 439 174 1721
Fragments arranged by size:
1721 439 174
SmaI CCC'GGG
Cuts at: 0 2059 2334
Size: 2059 275
SmlI C'TyrA_G
Cuts at: 0 1450 2334
Size: 1450 884
SphI G_CATG'C
Cuts at: 0 1550 2334
Size: 1550 784
SrfI GCCC'GGGC
Cuts at: 0 2059 2334
Size: 2059 275
StuI AGG'CCT
Cuts at: 0 52 219 528 2017 2334
Size: 52 167 309 1489 317
Fragments arranged by size:
1489 317 309 167 52
StyI C'CwwG_G
Cuts at: 0 299 323 1797 2018 2334
Size: 299 24 1474 221 316
Fragments arranged by size:
1474 316 299 221 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 655 1669 1686 1690 2334
Size: 655 1014 17 4 644
Fragments arranged by size:
1014 655 644 17 4
TatI w'GTAC_w
Cuts at: 0 1439 2334
Size: 1439 895
Tth111I GACn'n_nGTC
Cuts at: 0 1772 2334
Size: 1772 562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 459 2198 2334
Size: 459 1739 136
Fragments arranged by size:
1739 459 136
XmaI C'CCGG_G
Cuts at: 0 2057 2334
Size: 2057 277
Enzymes that do cut:
AcuI AfeI AflII AleI AloI AlwNI ApaI ApoI
AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtsI DraI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
MscI MslI MspA1I NspI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII ScaI SfcI SfiI SmaI SmlI
SphI SrfI StuI StyI TaqII TatI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AlfI ApaLI AscI AsiSI AvrII BbeI BcgI BciVI
BclI BfrBI BlpI BmtI BplI BpuEI BsaAI BsaWI
BsiEI BsiWI BsmBI BspEI BsrGI BssHII BstBI BstEII
BstZ17I BtgI BtgZI ClaI CspCI DraIII EagI EciI
EcoICRI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfoI
SgfI SgrAI SnaBI SpeI SspI SwaI TstI XbaI
XhoI XmnI ZraI