Restriction map for
pF1KB5673
(Linear) (Six-base) MAPSORT of: KIBB5673.seq Check: 4490 from: 1 to: 1650
KIBB5673.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 580 1650
Size: 580 1070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1053 1650
Size: 1053 597
AfeI AGC'GCT
Cuts at: 0 1074 1650
Size: 1074 576
AflIII A'CryG_T
Cuts at: 0 428 1643 1650
Size: 428 1215 7
Fragments arranged by size:
1215 428 7
ApoI r'AATT_y
Cuts at: 0 538 837 1650
Size: 538 299 813
Fragments arranged by size:
813 538 299
AvaI C'yCGr_G
Cuts at: 0 827 1650
Size: 827 823
BanII G_rGCy'C
Cuts at: 0 1067 1650
Size: 1067 583
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 1650
Size: 77 1573
BbvCI CC'TCA_GC
Cuts at: 0 460 1650
Size: 460 1190
Bme1580I G_kGCm'C
Cuts at: 0 1038 1650
Size: 1038 612
BmgBI CAC'GTC
Cuts at: 0 1232 1573 1650
Size: 1232 341 77
Fragments arranged by size:
1232 341 77
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 359 1558 1650
Size: 359 1199 92
Fragments arranged by size:
1199 359 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1650
Size: 329 1321
Bpu10I CC'TnA_GC
Cuts at: 0 460 845 1650
Size: 460 385 805
Fragments arranged by size:
805 460 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1422 1541 1650
Size: 526 896 119 109
Fragments arranged by size:
896 526 119 109
BsaAI yAC'GTr
Cuts at: 0 1644 1650
Size: 1644 6
BsaBI GATnn'nnATC
Cuts at: 0 1332 1650
Size: 1332 318
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 1650
Size: 433 1217
BsiHKAI G_wGCw'C
Cuts at: 0 342 1424 1433 1650
Size: 342 1082 9 217
Fragments arranged by size:
1082 342 217 9
Bsp1286I G_dGCh'C
Cuts at: 0 342 1038 1067 1424 1433 1650
Size: 342 696 29 357 9 217
Fragments arranged by size:
696 357 342 217 29 9
BspHI T'CATG_A
Cuts at: 0 680 1650
Size: 680 970
BsrDI GCAATG_nn'
Cuts at: 0 1536 1650
Size: 1536 114
BsrFI r'CCGG_y
Cuts at: 0 32 1650
Size: 32 1618
BssSI C'ACGA_G
Cuts at: 0 1076 1650
Size: 1076 574
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1605 1628 1650
Size: 1605 23 22
Fragments arranged by size:
1605 23 22
Bsu36I CC'TnA_GG
Cuts at: 0 59 1650
Size: 59 1591
BtgI C'CryG_G
Cuts at: 0 666 1650
Size: 666 984
BtsI GCAGTG_nn'
Cuts at: 0 739 1155 1650
Size: 739 416 495
Fragments arranged by size:
739 495 416
EaeI y'GGCC_r
Cuts at: 0 1618 1650
Size: 1618 32
EarI CTCTTCn'nnn_
Cuts at: 0 5 667 1135 1429 1438 1650
Size: 5 662 468 294 9 212
Fragments arranged by size:
662 468 294 212 9 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1053 1650
Size: 329 724 597
Fragments arranged by size:
724 597 329
HaeII r_GCGC'y
Cuts at: 0 378 1076 1650
Size: 378 698 574
Fragments arranged by size:
698 574 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 627 642 659 674 1650
Size: 4 32 591 15 17 15 976
Fragments arranged by size:
976 591 32 17 15 15 4
HincII GTy'rAC
Cuts at: 0 161 882 1650
Size: 161 721 768
Fragments arranged by size:
768 721 161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1265 1459 1650
Size: 1265 194 191
Fragments arranged by size:
1265 194 191
MslI CAynn'nnrTG
Cuts at: 0 218 1382 1650
Size: 218 1164 268
Fragments arranged by size:
1164 268 218
MspA1I CmG'CkG
Cuts at: 0 648 1650
Size: 648 1002
NcoI C'CATG_G
Cuts at: 0 666 1650
Size: 666 984
NdeI CA'TA_TG
Cuts at: 0 720 1650
Size: 720 930
NspI r_CATG'y
Cuts at: 0 432 1650
Size: 432 1218
PciI A'CATG_T
Cuts at: 0 428 1650
Size: 428 1222
PfoI T'CCnGG_A
Cuts at: 0 1627 1650
Size: 1627 23
SapI GCTCTTCn'nnn_
Cuts at: 0 1429 1438 1650
Size: 1429 9 212
Fragments arranged by size:
1429 212 9
SexAI A'CCwGG_T
Cuts at: 0 559 1650
Size: 559 1091
SfcI C'TryA_G
Cuts at: 0 627 875 1370 1650
Size: 627 248 495 280
Fragments arranged by size:
627 495 280 248
SmlI C'TyrA_G
Cuts at: 0 505 1401 1556 1650
Size: 505 896 155 94
Fragments arranged by size:
896 505 155 94
StyI C'CwwG_G
Cuts at: 0 666 1388 1650
Size: 666 722 262
Fragments arranged by size:
722 666 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 260 1650
Size: 260 1390
TatI w'GTAC_w
Cuts at: 0 893 1010 1374 1650
Size: 893 117 364 276
Fragments arranged by size:
893 364 276 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 1650
Size: 485 32 1133
Fragments arranged by size:
1133 485 32
Enzymes that do cut:
AccI AcuI AfeI AflIII ApoI AvaI BanII BbsI
BbvCI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BseRI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BssSI
BstXI Bsu36I BtgI BtsI EaeI EarI Eco57MI HaeII
Hin4I HincII MmeI MslI MspA1I NcoI NdeI NspI
PciI PfoI SapI SexAI SfcI SmlI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BsaI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI