Restriction map for
pF1KB5672
(Linear) (Six-base) MAPSORT of: KIBB5672.seq Check: 513 from: 1 to: 1608
KIBB5672.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1244 1608
Size: 1244 364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 418 768 1144 1608
Size: 92 326 350 376 464
Fragments arranged by size:
464 376 350 326 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 831 1608
Size: 831 777
AlwNI CAG_nnn'CTG
Cuts at: 0 502 1608
Size: 502 1106
ApaI G_GGCC'C
Cuts at: 0 699 825 1608
Size: 699 126 783
Fragments arranged by size:
783 699 126
ApoI r'AATT_y
Cuts at: 0 307 1608
Size: 307 1301
AseI AT'TA_AT
Cuts at: 0 167 1608
Size: 167 1441
BanI G'GyrC_C
Cuts at: 0 1244 1608
Size: 1244 364
BanII G_rGCy'C
Cuts at: 0 19 220 444 699 825 1107 1608
Size: 19 201 224 255 126 282 501
Fragments arranged by size:
501 282 255 224 201 126 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 187 1520 1608
Size: 187 1333 88
Fragments arranged by size:
1333 187 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1293 1327 1608
Size: 1293 34 281
Fragments arranged by size:
1293 281 34
BclI T'GATC_A
Cuts at: 0 239 1608
Size: 239 1369
BglII A'GATC_T
Cuts at: 0 1466 1608
Size: 1466 142
Bme1580I G_kGCm'C
Cuts at: 0 570 699 825 1608
Size: 570 129 126 783
Fragments arranged by size:
783 570 129 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 236 580 977 1267 1608
Size: 236 344 397 290 341
Fragments arranged by size:
397 344 341 290 236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1354 1429 1608
Size: 605 749 75 179
Fragments arranged by size:
749 605 179 75
Bpu10I CC'TnA_GC
Cuts at: 0 1232 1380 1608
Size: 1232 148 228
Fragments arranged by size:
1232 228 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 753 1257 1608
Size: 753 504 351
Fragments arranged by size:
753 504 351
BsaBI GATnn'nnATC
Cuts at: 0 1153 1608
Size: 1153 455
BsaWI w'CCGG_w
Cuts at: 0 113 311 877 1055 1608
Size: 113 198 566 178 553
Fragments arranged by size:
566 553 198 178 113
BseYI C'CCAG_C
Cuts at: 0 818 1608
Size: 818 790
BsiHKAI G_wGCw'C
Cuts at: 0 220 1499 1608
Size: 220 1279 109
Fragments arranged by size:
1279 220 109
BsmI GAATG_Cn'
Cuts at: 0 104 690 1608
Size: 104 586 918
Fragments arranged by size:
918 586 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 75 1608
Size: 75 1533
Bsp1286I G_dGCh'C
Cuts at: 0 19 220 444 570 699 825 1107 1499
Size: 19 201 224 126 129 126 282 392
Cuts at: 1499 1608
Size: 109
Fragments arranged by size:
392 282 224 201 129 126 126 109
19
BspEI T'CCGG_A
Cuts at: 0 113 311 877 1055 1608
Size: 113 198 566 178 553
Fragments arranged by size:
566 553 198 178 113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 1608
Size: 293 1315
BsrGI T'GTAC_A
Cuts at: 0 415 1608
Size: 415 1193
BssSI C'ACGA_G
Cuts at: 0 1453 1608
Size: 1453 155
BstBI TT'CG_AA
Cuts at: 0 1303 1608
Size: 1303 305
BstYI r'GATC_y
Cuts at: 0 857 1466 1608
Size: 857 609 142
Fragments arranged by size:
857 609 142
Bsu36I CC'TnA_GG
Cuts at: 0 1580 1608
Size: 1580 28
BtgI C'CryG_G
Cuts at: 0 8 950 1589 1608
Size: 8 942 639 19
Fragments arranged by size:
942 639 19 8
BtsI GCAGTG_nn'
Cuts at: 0 1380 1608
Size: 1380 228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 356 391 1356 1391 1608
Size: 356 35 965 35 217
Fragments arranged by size:
965 356 217 35 35
DraI TTT'AAA
Cuts at: 0 144 1608
Size: 144 1464
DrdI GACnn_nn'nnGTC
Cuts at: 0 880 1608
Size: 880 728
EarI CTCTTCn'nnn_
Cuts at: 0 37 84 92 394 787 1608
Size: 37 47 8 302 393 821
Fragments arranged by size:
821 393 302 47 37 8
EcoICRI GAG'CTC
Cuts at: 0 218 1608
Size: 218 1390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 418 605 768 1144 1354 1429 1608
Size: 92 326 187 163 376 210 75 179
Fragments arranged by size:
376 326 210 187 179 163 92 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1408 1608
Size: 1408 200
EcoO109I rG'GnC_Cy
Cuts at: 0 696 822 1584 1608
Size: 696 126 762 24
Fragments arranged by size:
762 696 126 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 596 628 1608
Size: 596 32 980
Fragments arranged by size:
980 596 32
HincII GTy'rAC
Cuts at: 0 644 1608
Size: 644 964
HindIII A'AGCT_T
Cuts at: 0 1064 1608
Size: 1064 544
HpaI GTT'AAC
Cuts at: 0 644 1608
Size: 644 964
KpnI G_GTAC'C
Cuts at: 0 1248 1608
Size: 1248 360
NcoI C'CATG_G
Cuts at: 0 8 1589 1608
Size: 8 1581 19
Fragments arranged by size:
1581 19 8
PasI CC'CwG_GG
Cuts at: 0 19 1608
Size: 19 1589
PshAI GACnn'nnGTC
Cuts at: 0 988 1608
Size: 988 620
PspOMI G'GGCC_C
Cuts at: 0 695 821 1608
Size: 695 126 787
Fragments arranged by size:
787 695 126
PstI C_TGCA'G
Cuts at: 0 290 1608
Size: 290 1318
SacI G_AGCT'C
Cuts at: 0 220 1608
Size: 220 1388
SapI GCTCTTCn'nnn_
Cuts at: 0 37 1608
Size: 37 1571
SexAI A'CCwGG_T
Cuts at: 0 1247 1608
Size: 1247 361
SfcI C'TryA_G
Cuts at: 0 286 1608
Size: 286 1322
SspI AAT'ATT
Cuts at: 0 453 1086 1608
Size: 453 633 522
Fragments arranged by size:
633 522 453
StuI AGG'CCT
Cuts at: 0 1480 1608
Size: 1480 128
StyI C'CwwG_G
Cuts at: 0 8 1043 1589 1608
Size: 8 1035 546 19
Fragments arranged by size:
1035 546 19 8
TatI w'GTAC_w
Cuts at: 0 415 765 1608
Size: 415 350 843
Fragments arranged by size:
843 415 350
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 293 325 1608
Size: 293 32 1283
Fragments arranged by size:
1283 293 32
XmnI GAAnn'nnTTC
Cuts at: 0 1157 1480 1608
Size: 1157 323 128
Fragments arranged by size:
1157 323 128
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI ApoI AseI BanI
BanII BbsI BcgI BclI BglII Bme1580I BmrI BpmI
Bpu10I BsaI BsaBI BsaWI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrGI BssSI BstBI BstYI Bsu36I
BtgI BtsI CspCI DraI DrdI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HindIII HpaI KpnI NcoI
PasI PshAI PspOMI PstI SacI SapI SexAI SfcI
SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaHI BsaXI BseRI
BsgI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI BssHII
BstAPI BstEII BstXI BstZ17I BtgZI ClaI DraIII EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII KasI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI