Restriction map for
pF1KB5654
(Linear) (Six-base) MAPSORT of: KIBB5654.seq Check: 6998 from: 1 to: 993
KIBB5654.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 0 147 993
Size: 147 846
AccI GT'mk_AC
Cuts at: 0 797 993
Size: 797 196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 475 993
Size: 404 71 518
Fragments arranged by size:
518 404 71
AflIII A'CryG_T
Cuts at: 0 667 993
Size: 667 326
AlwNI CAG_nnn'CTG
Cuts at: 0 498 745 993
Size: 498 247 248
Fragments arranged by size:
498 248 247
ApaI G_GGCC'C
Cuts at: 0 441 993
Size: 441 552
BanII G_rGCy'C
Cuts at: 0 441 993
Size: 441 552
Bme1580I G_kGCm'C
Cuts at: 0 441 492 993
Size: 441 51 501
Fragments arranged by size:
501 441 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 733 993
Size: 733 260
BmtI G_CTAG'C
Cuts at: 0 971 993
Size: 971 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 381 993
Size: 211 170 612
Fragments arranged by size:
612 211 170
BsaHI Gr'CG_yC
Cuts at: 0 144 993
Size: 144 849
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 993
Size: 24 969
BseYI C'CCAG_C
Cuts at: 0 292 416 896 993
Size: 292 124 480 97
Fragments arranged by size:
480 292 124 97
BsiHKAI G_wGCw'C
Cuts at: 0 23 34 993
Size: 23 11 959
Fragments arranged by size:
959 23 11
BsmI GAATG_Cn'
Cuts at: 0 195 212 993
Size: 195 17 781
Fragments arranged by size:
781 195 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 151 993
Size: 151 842
Bsp1286I G_dGCh'C
Cuts at: 0 23 34 441 492 993
Size: 23 11 407 51 501
Fragments arranged by size:
501 407 51 23 11
BspHI T'CATG_A
Cuts at: 0 551 993
Size: 551 442
BsrDI GCAATG_nn'
Cuts at: 0 546 993
Size: 546 447
BstXI CCAn_nnnn'nTGG
Cuts at: 0 910 993
Size: 910 83
BstYI r'GATC_y
Cuts at: 0 60 993
Size: 60 933
BtgI C'CryG_G
Cuts at: 0 623 993
Size: 623 370
BtsI GCAGTG_nn'
Cuts at: 0 47 993
Size: 47 946
EaeI y'GGCC_r
Cuts at: 0 725 993
Size: 725 268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 381 404 475 993
Size: 211 170 23 71 518
Fragments arranged by size:
518 211 170 71 23
EcoO109I rG'GnC_Cy
Cuts at: 0 437 438 993
Size: 437 1 555
Fragments arranged by size:
555 437 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 692 724 993
Size: 134 32 526 32 269
Fragments arranged by size:
526 269 134 32 32
MscI TGG'CCA
Cuts at: 0 727 993
Size: 727 266
MspA1I CmG'CkG
Cuts at: 0 453 896 993
Size: 453 443 97
Fragments arranged by size:
453 443 97
NcoI C'CATG_G
Cuts at: 0 623 993
Size: 623 370
NheI G'CTAG_C
Cuts at: 0 967 993
Size: 967 26
NspI r_CATG'y
Cuts at: 0 671 993
Size: 671 322
PciI A'CATG_T
Cuts at: 0 667 993
Size: 667 326
PflMI CCAn_nnn'nTGG
Cuts at: 0 299 498 993
Size: 299 199 495
Fragments arranged by size:
495 299 199
PspOMI G'GGCC_C
Cuts at: 0 437 993
Size: 437 556
PstI C_TGCA'G
Cuts at: 0 42 993
Size: 42 951
PvuII CAG'CTG
Cuts at: 0 453 896 993
Size: 453 443 97
Fragments arranged by size:
453 443 97
SexAI A'CCwGG_T
Cuts at: 0 497 993
Size: 497 496
SfcI C'TryA_G
Cuts at: 0 38 783 993
Size: 38 745 210
Fragments arranged by size:
745 210 38
StyI C'CwwG_G
Cuts at: 0 623 993
Size: 623 370
TatI w'GTAC_w
Cuts at: 0 569 677 814 837 993
Size: 569 108 137 23 156
Fragments arranged by size:
569 156 137 108 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 461 493 672 704 993
Size: 461 32 179 32 289
Fragments arranged by size:
461 289 179 32 32
ZraI GAC'GTC
Cuts at: 0 145 993
Size: 145 848
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI BanII Bme1580I
BmrI BmtI BpmI BsaHI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrDI BstXI BstYI BtgI BtsI
EaeI Eco57MI EcoO109I Hin4I MscI MspA1I NcoI NheI
NspI PciI PflMI PspOMI PstI PvuII SexAI SfcI
StyI TatI TstI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiEI BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI