Restriction map for
pF1KB5647
(Linear) (Six-base) MAPSORT of: KIBB5647.seq Check: 4237 from: 1 to: 951
KIBB5647.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 718 951
Size: 441 277 233
Fragments arranged by size:
441 277 233
AhdI GACnn_n'nnGTC
Cuts at: 0 598 951
Size: 598 353
ApaI G_GGCC'C
Cuts at: 0 589 951
Size: 589 362
ApoI r'AATT_y
Cuts at: 0 337 723 829 951
Size: 337 386 106 122
Fragments arranged by size:
386 337 122 106
AseI AT'TA_AT
Cuts at: 0 914 951
Size: 914 37
AvaI C'yCGr_G
Cuts at: 0 173 951
Size: 173 778
BamHI G'GATC_C
Cuts at: 0 81 951
Size: 81 870
BanI G'GyrC_C
Cuts at: 0 61 951
Size: 61 890
BanII G_rGCy'C
Cuts at: 0 589 890 951
Size: 589 301 61
Fragments arranged by size:
589 301 61
BglI GCCn_nnn'nGGC
Cuts at: 0 184 951
Size: 184 767
BlpI GC'TnA_GC
Cuts at: 0 547 889 951
Size: 547 342 62
Fragments arranged by size:
547 342 62
Bme1580I G_kGCm'C
Cuts at: 0 589 951
Size: 589 362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 951
Size: 883 68
BseYI C'CCAG_C
Cuts at: 0 542 951
Size: 542 409
BsiHKAI G_wGCw'C
Cuts at: 0 890 951
Size: 890 61
BsmI GAATG_Cn'
Cuts at: 0 627 951
Size: 627 324
Bsp1286I G_dGCh'C
Cuts at: 0 589 890 951
Size: 589 301 61
Fragments arranged by size:
589 301 61
BsrFI r'CCGG_y
Cuts at: 0 217 951
Size: 217 734
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 951
Size: 230 721
BstYI r'GATC_y
Cuts at: 0 81 144 951
Size: 81 63 807
Fragments arranged by size:
807 81 63
BtgI C'CryG_G
Cuts at: 0 581 951
Size: 581 370
BtsI GCAGTG_nn'
Cuts at: 0 168 951
Size: 168 783
DraI TTT'AAA
Cuts at: 0 882 951
Size: 882 69
EaeI y'GGCC_r
Cuts at: 0 378 951
Size: 378 573
EarI CTCTTCn'nnn_
Cuts at: 0 260 749 951
Size: 260 489 202
Fragments arranged by size:
489 260 202
EcoICRI GAG'CTC
Cuts at: 0 888 951
Size: 888 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 718 883 951
Size: 441 277 165 68
Fragments arranged by size:
441 277 165 68
EcoO109I rG'GnC_Cy
Cuts at: 0 586 951
Size: 586 365
EcoRI G'AATT_C
Cuts at: 0 829 951
Size: 829 122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 743 951
Size: 743 208
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 584 616 951
Size: 584 32 335
Fragments arranged by size:
584 335 32
HincII GTy'rAC
Cuts at: 0 648 951
Size: 648 303
HindIII A'AGCT_T
Cuts at: 0 636 951
Size: 636 315
MfeI C'AATT_G
Cuts at: 0 443 951
Size: 443 508
MscI TGG'CCA
Cuts at: 0 380 951
Size: 380 571
MslI CAynn'nnrTG
Cuts at: 0 580 951
Size: 580 371
MspA1I CmG'CkG
Cuts at: 0 78 951
Size: 78 873
NaeI GCC'GGC
Cuts at: 0 219 951
Size: 219 732
NcoI C'CATG_G
Cuts at: 0 581 951
Size: 581 370
NgoMIV G'CCGG_C
Cuts at: 0 217 951
Size: 217 734
NspI r_CATG'y
Cuts at: 0 115 951
Size: 115 836
PflMI CCAn_nnn'nTGG
Cuts at: 0 532 933 951
Size: 532 401 18
Fragments arranged by size:
532 401 18
PfoI T'CCnGG_A
Cuts at: 0 84 951
Size: 84 867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 951
Size: 506 32 413
Fragments arranged by size:
506 413 32
PspOMI G'GGCC_C
Cuts at: 0 585 951
Size: 585 366
PspXI vC'TCGA_Gb
Cuts at: 0 173 951
Size: 173 778
PstI C_TGCA'G
Cuts at: 0 152 951
Size: 152 799
PvuII CAG'CTG
Cuts at: 0 78 951
Size: 78 873
SacI G_AGCT'C
Cuts at: 0 890 951
Size: 890 61
SfcI C'TryA_G
Cuts at: 0 22 148 951
Size: 22 126 803
Fragments arranged by size:
803 126 22
SmlI C'TyrA_G
Cuts at: 0 173 951
Size: 173 778
SspI AAT'ATT
Cuts at: 0 435 705 765 951
Size: 435 270 60 186
Fragments arranged by size:
435 270 186 60
StyI C'CwwG_G
Cuts at: 0 581 951
Size: 581 370
TatI w'GTAC_w
Cuts at: 0 794 866 951
Size: 794 72 85
Fragments arranged by size:
794 85 72
XhoI C'TCGA_G
Cuts at: 0 173 951
Size: 173 778
XmnI GAAnn'nnTTC
Cuts at: 0 521 951
Size: 521 430
Enzymes that do cut:
AcuI AhdI ApaI ApoI AseI AvaI BamHI BanI
BanII BglI BlpI Bme1580I BpmI BseYI BsiHKAI BsmI
Bsp1286I BsrFI BstAPI BstYI BtgI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII
HindIII MfeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PfoI PpiI PspOMI PspXI PstI PvuII
SacI SfcI SmlI SspI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI AscI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XmaI ZraI