Restriction map for
pF1KB5641
(Linear) (Six-base) MAPSORT of: KIBB5641.seq Check: 5679 from: 1 to: 2277
KIBB5641.orf
With 182 enzymes: SgfI *
March 31, 2008 15:57 ..
AatII G_ACGT'C
Cuts at: 0 77 2000 2277
Size: 77 1923 277
Fragments arranged by size:
1923 277 77
AccI GT'mk_AC
Cuts at: 0 1802 2277
Size: 1802 475
AclI AA'CG_TT
Cuts at: 0 790 2277
Size: 790 1487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 2277
Size: 674 1603
AfeI AGC'GCT
Cuts at: 0 1673 2038 2277
Size: 1673 365 239
Fragments arranged by size:
1673 365 239
AflIII A'CryG_T
Cuts at: 0 1409 2081 2277
Size: 1409 672 196
Fragments arranged by size:
1409 672 196
AhdI GACnn_n'nnGTC
Cuts at: 0 1878 2277
Size: 1878 399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1486 2277
Size: 1486 791
AlwNI CAG_nnn'CTG
Cuts at: 0 264 756 1123 2277
Size: 264 492 367 1154
Fragments arranged by size:
1154 492 367 264
ApaLI G'TGCA_C
Cuts at: 0 734 2277
Size: 734 1543
ApoI r'AATT_y
Cuts at: 0 1565 2277
Size: 1565 712
AseI AT'TA_AT
Cuts at: 0 1088 2277
Size: 1088 1189
AvaI C'yCGr_G
Cuts at: 0 435 2277
Size: 435 1842
BamHI G'GATC_C
Cuts at: 0 246 746 2277
Size: 246 500 1531
Fragments arranged by size:
1531 500 246
BanI G'GyrC_C
Cuts at: 0 759 2277
Size: 759 1518
BanII G_rGCy'C
Cuts at: 0 370 419 2277
Size: 370 49 1858
Fragments arranged by size:
1858 370 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 662 2277
Size: 662 1615
BbvCI CC'TCA_GC
Cuts at: 0 510 2277
Size: 510 1767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2053 2087 2277
Size: 2053 34 190
Fragments arranged by size:
2053 190 34
BclI T'GATC_A
Cuts at: 0 849 2277
Size: 849 1428
BfrBI ATG'CAT
Cuts at: 0 1177 2277
Size: 1177 1100
BglII A'GATC_T
Cuts at: 0 498 978 1631 2277
Size: 498 480 653 646
Fragments arranged by size:
653 646 498 480
BlpI GC'TnA_GC
Cuts at: 0 1438 2277
Size: 1438 839
Bme1580I G_kGCm'C
Cuts at: 0 738 2277
Size: 738 1539
BmrI ACTGGGnnnn_n'
Cuts at: 0 636 1133 2277
Size: 636 497 1144
Fragments arranged by size:
1144 636 497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1653 2277
Size: 1653 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1444 2277
Size: 967 477 833
Fragments arranged by size:
967 833 477
Bpu10I CC'TnA_GC
Cuts at: 0 364 510 2277
Size: 364 146 1767
Fragments arranged by size:
1767 364 146
BsaAI yAC'GTr
Cuts at: 0 1776 2277
Size: 1776 501
BsaHI Gr'CG_yC
Cuts at: 0 74 1997 2277
Size: 74 1923 280
Fragments arranged by size:
1923 280 74
BsaWI w'CCGG_w
Cuts at: 0 743 2277
Size: 743 1534
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1008 1038 2277
Size: 1008 30 1239
Fragments arranged by size:
1239 1008 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 787 2277
Size: 787 1490
BseYI C'CCAG_C
Cuts at: 0 93 267 458 569 814 972 2277
Size: 93 174 191 111 245 158 1305
Fragments arranged by size:
1305 245 191 174 158 111 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 724 1456 1798 2116 2277
Size: 239 485 732 342 318 161
Fragments arranged by size:
732 485 342 318 239 161
BsiHKAI G_wGCw'C
Cuts at: 0 738 2277
Size: 738 1539
BsmI GAATG_Cn'
Cuts at: 0 1668 2277
Size: 1668 609
BsmBI CGTCTCn'nnnn_
Cuts at: 0 969 2277
Size: 969 1308
Bsp1286I G_dGCh'C
Cuts at: 0 370 419 738 2277
Size: 370 49 319 1539
Fragments arranged by size:
1539 370 319 49
BsrDI GCAATG_nn'
Cuts at: 0 905 1610 2277
Size: 905 705 667
Fragments arranged by size:
905 705 667
BssSI C'ACGA_G
Cuts at: 0 1627 2277
Size: 1627 650
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1647 1674 2277
Size: 1647 27 603
Fragments arranged by size:
1647 603 27
BstYI r'GATC_y
Cuts at: 0 246 498 746 978 1631 2277
Size: 246 252 248 232 653 646
Fragments arranged by size:
653 646 252 248 246 232
BtgI C'CryG_G
Cuts at: 0 233 546 1051 1454 2277
Size: 233 313 505 403 823
Fragments arranged by size:
823 505 403 313 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 782 2277
Size: 782 1495
BtsI GCAGTG_nn'
Cuts at: 0 50 371 736 2277
Size: 50 321 365 1541
Fragments arranged by size:
1541 365 321 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 582 617 2277
Size: 582 35 1660
Fragments arranged by size:
1660 582 35
DraI TTT'AAA
Cuts at: 0 1145 2277
Size: 1145 1132
DrdI GACnn_nn'nnGTC
Cuts at: 0 792 1810 2277
Size: 792 1018 467
Fragments arranged by size:
1018 792 467
EaeI y'GGCC_r
Cuts at: 0 53 230 660 1524 2277
Size: 53 177 430 864 753
Fragments arranged by size:
864 753 430 177 53
EarI CTCTTCn'nnn_
Cuts at: 0 554 1289 1931 2190 2277
Size: 554 735 642 259 87
Fragments arranged by size:
735 642 554 259 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 967 1444 2277
Size: 674 293 477 833
Fragments arranged by size:
833 674 477 293
EcoO109I rG'GnC_Cy
Cuts at: 0 348 914 1299 2277
Size: 348 566 385 978
Fragments arranged by size:
978 566 385 348
EcoRV GAT'ATC
Cuts at: 0 2119 2277
Size: 2119 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1021 2277
Size: 1021 1256
HaeII r_GCGC'y
Cuts at: 0 1675 2040 2277
Size: 1675 365 237
Fragments arranged by size:
1675 365 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 793 825 1367 1399 1864 1896
Size: 760 32 1 32 542 32 465 32
Cuts at: 1896 2277
Size: 381
Fragments arranged by size:
760 542 465 381 32 32 32 32
1
HincII GTy'rAC
Cuts at: 0 33 1128 1332 2277
Size: 33 1095 204 945
Fragments arranged by size:
1095 945 204 33
HpaI GTT'AAC
Cuts at: 0 1128 2277
Size: 1128 1149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1765 1794 1886 2277
Size: 1765 29 92 391
Fragments arranged by size:
1765 391 92 29
MscI TGG'CCA
Cuts at: 0 55 2277
Size: 55 2222
MslI CAynn'nnrTG
Cuts at: 0 1208 1498 1894 2269 2277
Size: 1208 290 396 375 8
Fragments arranged by size:
1208 396 375 290 8
MspA1I CmG'CkG
Cuts at: 0 19 62 253 782 2277
Size: 19 43 191 529 1495
Fragments arranged by size:
1495 529 191 43 19
NcoI C'CATG_G
Cuts at: 0 233 546 1051 1454 2277
Size: 233 313 505 403 823
Fragments arranged by size:
823 505 403 313 233
NsiI A_TGCA'T
Cuts at: 0 1179 2277
Size: 1179 1098
NspI r_CATG'y
Cuts at: 0 1117 1413 1437 1794 2112 2277
Size: 1117 296 24 357 318 165
Fragments arranged by size:
1117 357 318 296 165 24
PciI A'CATG_T
Cuts at: 0 1409 2277
Size: 1409 868
PflMI CCAn_nnn'nTGG
Cuts at: 0 756 2277
Size: 756 1521
PfoI T'CCnGG_A
Cuts at: 0 1421 2277
Size: 1421 856
PmlI CAC'GTG
Cuts at: 0 1776 2277
Size: 1776 501
PpuMI rG'GwC_Cy
Cuts at: 0 348 914 1299 2277
Size: 348 566 385 978
Fragments arranged by size:
978 566 385 348
PshAI GACnn'nnGTC
Cuts at: 0 642 2277
Size: 642 1635
PspXI vC'TCGA_Gb
Cuts at: 0 435 2277
Size: 435 1842
PstI C_TGCA'G
Cuts at: 0 333 378 626 1684 2277
Size: 333 45 248 1058 593
Fragments arranged by size:
1058 593 333 248 45
PvuII CAG'CTG
Cuts at: 0 19 253 782 2277
Size: 19 234 529 1495
Fragments arranged by size:
1495 529 234 19
ScaI AGT'ACT
Cuts at: 0 11 1783 2277
Size: 11 1772 494
Fragments arranged by size:
1772 494 11
SfcI C'TryA_G
Cuts at: 0 68 329 374 622 1680 1799 2277
Size: 68 261 45 248 1058 119 478
Fragments arranged by size:
1058 478 261 248 119 68 45
SmlI C'TyrA_G
Cuts at: 0 435 2277
Size: 435 1842
SphI G_CATG'C
Cuts at: 0 1117 2277
Size: 1117 1160
StuI AGG'CCT
Cuts at: 0 589 2277
Size: 589 1688
StyI C'CwwG_G
Cuts at: 0 38 233 534 546 1051 1454 2277
Size: 38 195 301 12 505 403 823
Fragments arranged by size:
823 505 403 301 195 38 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 437 828 1972 2277
Size: 437 391 1144 305
Fragments arranged by size:
1144 437 391 305
TatI w'GTAC_w
Cuts at: 0 9 1781 2277
Size: 9 1772 496
Fragments arranged by size:
1772 496 9
Tth111I GACn'n_nGTC
Cuts at: 0 1381 2277
Size: 1381 896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 291 2277
Size: 291 1986
XhoI C'TCGA_G
Cuts at: 0 435 2277
Size: 435 1842
XmnI GAAnn'nnTTC
Cuts at: 0 11 2277
Size: 11 2266
ZraI GAC'GTC
Cuts at: 0 75 1998 2277
Size: 75 1923 279
Fragments arranged by size:
1923 279 75
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflIII AhdI AlfI
AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI
BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI
NsiI NspI PciI PflMI PfoI PmlI PpuMI PshAI
PspXI PstI PvuII ScaI SfcI SmlI SphI StuI
StyI TaqII TatI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AflII AgeI AleI AloI ApaI AscI
AsiSI AvrII BaeI BbeI BciVI BglI BmgBI BmtI
BpuEI BsaI BsaBI BsiEI BsiWI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraIII EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PpiI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TstI XbaI XmaI