Restriction map for
pF1KB5636
(Linear) (Six-base) MAPSORT of: KIBB5636.seq Check: 271 from: 1 to: 993
KIBB5636.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 429 993
Size: 429 564
ApaI G_GGCC'C
Cuts at: 0 296 993
Size: 296 697
AscI GG'CGCG_CC
Cuts at: 0 821 993
Size: 821 172
AvaI C'yCGr_G
Cuts at: 0 177 289 993
Size: 177 112 704
Fragments arranged by size:
704 177 112
BanI G'GyrC_C
Cuts at: 0 205 261 748 993
Size: 205 56 487 245
Fragments arranged by size:
487 245 205 56
BanII G_rGCy'C
Cuts at: 0 146 296 629 923 993
Size: 146 150 333 294 70
Fragments arranged by size:
333 294 150 146 70
BbeI G_GCGC'C
Cuts at: 0 209 993
Size: 209 784
BbvCI CC'TCA_GC
Cuts at: 0 418 993
Size: 418 575
BglI GCCn_nnn'nGGC
Cuts at: 0 76 182 993
Size: 76 106 811
Fragments arranged by size:
811 106 76
BlpI GC'TnA_GC
Cuts at: 0 667 993
Size: 667 326
Bme1580I G_kGCm'C
Cuts at: 0 266 296 751 993
Size: 266 30 455 242
Fragments arranged by size:
455 266 242 30
BmgBI CAC'GTC
Cuts at: 0 849 993
Size: 849 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 142 169 993
Size: 142 27 824
Fragments arranged by size:
824 142 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 993
Size: 849 144
Bpu10I CC'TnA_GC
Cuts at: 0 418 993
Size: 418 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 993
Size: 501 492
BsaHI Gr'CG_yC
Cuts at: 0 107 206 993
Size: 107 99 787
Fragments arranged by size:
787 107 99
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 519 754 757 931 993
Size: 519 235 3 174 62
Fragments arranged by size:
519 235 174 62 3
BseYI C'CCAG_C
Cuts at: 0 41 752 993
Size: 41 711 241
Fragments arranged by size:
711 241 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 376 993
Size: 300 76 617
Fragments arranged by size:
617 300 76
BsiEI CG_ry'CG
Cuts at: 0 13 65 134 245 348 399 993
Size: 13 52 69 111 103 51 594
Fragments arranged by size:
594 111 103 69 52 51 13
BsiHKAI G_wGCw'C
Cuts at: 0 629 993
Size: 629 364
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 993
Size: 112 881
Bsp1286I G_dGCh'C
Cuts at: 0 146 266 296 629 751 923 993
Size: 146 120 30 333 122 172 70
Fragments arranged by size:
333 172 146 122 120 70 30
BsrBI CCG'CTC
Cuts at: 0 9 993
Size: 9 984
BsrFI r'CCGG_y
Cuts at: 0 193 982 993
Size: 193 789 11
Fragments arranged by size:
789 193 11
BssHII G'CGCG_C
Cuts at: 0 164 166 250 401 821 993
Size: 164 2 84 151 420 172
Fragments arranged by size:
420 172 164 151 84 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 849 993
Size: 849 144
BstYI r'GATC_y
Cuts at: 0 680 993
Size: 680 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 603 993
Size: 603 390
BtsI GCAGTG_nn'
Cuts at: 0 39 993
Size: 39 954
EaeI y'GGCC_r
Cuts at: 0 10 62 131 242 345 396 692 716
Size: 10 52 69 111 103 51 296 24
Cuts at: 716 993
Size: 277
Fragments arranged by size:
296 277 111 103 69 52 51 24
10
EagI C'GGCC_G
Cuts at: 0 10 62 131 242 345 396 993
Size: 10 52 69 111 103 51 597
Fragments arranged by size:
597 111 103 69 52 51 10
EarI CTCTTCn'nnn_
Cuts at: 0 496 993
Size: 496 497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 993
Size: 676 317
EcoICRI GAG'CTC
Cuts at: 0 627 993
Size: 627 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 993
Size: 849 144
EcoO109I rG'GnC_Cy
Cuts at: 0 614 774 993
Size: 614 160 219
Fragments arranged by size:
614 219 160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 868 993
Size: 868 125
HaeII r_GCGC'y
Cuts at: 0 209 993
Size: 209 784
KasI G'GCGC_C
Cuts at: 0 205 993
Size: 205 788
MscI TGG'CCA
Cuts at: 0 694 718 993
Size: 694 24 275
Fragments arranged by size:
694 275 24
MspA1I CmG'CkG
Cuts at: 0 139 328 360 429 446 756 869 993
Size: 139 189 32 69 17 310 113 124
Fragments arranged by size:
310 189 139 124 113 69 32 17
NaeI GCC'GGC
Cuts at: 0 984 993
Size: 984 9
NarI GG'CG_CC
Cuts at: 0 206 993
Size: 206 787
NgoMIV G'CCGG_C
Cuts at: 0 982 993
Size: 982 11
NotI GC'GGCC_GC
Cuts at: 0 10 131 993
Size: 10 121 862
Fragments arranged by size:
862 121 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 993
Size: 429 564
PpuMI rG'GwC_Cy
Cuts at: 0 774 993
Size: 774 219
PspOMI G'GGCC_C
Cuts at: 0 292 993
Size: 292 701
PvuII CAG'CTG
Cuts at: 0 360 869 993
Size: 360 509 124
Fragments arranged by size:
509 360 124
SacI G_AGCT'C
Cuts at: 0 629 993
Size: 629 364
SexAI A'CCwGG_T
Cuts at: 0 89 993
Size: 89 904
SfcI C'TryA_G
Cuts at: 0 411 993
Size: 411 582
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 76 993
Size: 76 917
SfoI GGC'GCC
Cuts at: 0 207 993
Size: 207 786
SmaI CCC'GGG
Cuts at: 0 179 291 993
Size: 179 112 702
Fragments arranged by size:
702 179 112
StyI C'CwwG_G
Cuts at: 0 113 993
Size: 113 880
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 766 993
Size: 19 747 227
Fragments arranged by size:
747 227 19
XmaI C'CCGG_G
Cuts at: 0 177 289 993
Size: 177 112 704
Fragments arranged by size:
704 177 112
Enzymes that do cut:
AlwNI ApaI AscI AvaI BanI BanII BbeI BbvCI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrBI BsrFI BssHII BstAPI BstYI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII
KasI MscI MspA1I NaeI NarI NgoMIV NotI PflMI
PpuMI PspOMI PvuII SacI SexAI SfcI SfiI SfoI
SmaI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglII BmtI BplI BpuEI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MslI NcoI NdeI NheI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI