(Linear) (Six-base) MAPSORT of: KIBB5603.seq  Check: 4363  from: 1  to: 1398



KIBB5603.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     536    1398 
   Size:         89     447     862

  Fragments arranged by size: 

                 862     447      89
 
AflIII A'CryG_T

Cuts at:      0     354     786    1398 
   Size:        354     432     612

  Fragments arranged by size: 

                 612     432     354
 
ApoI r'AATT_y

Cuts at:      0     160     859    1000    1398 
   Size:        160     699     141     398

  Fragments arranged by size: 

                 699     398     160     141
 
AvaI C'yCGr_G

Cuts at:      0     293    1398 
   Size:        293    1105
 
BanI G'GyrC_C

Cuts at:      0    1112    1398 
   Size:       1112     286
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     140     435     755    1398 
   Size:        140     295     320     643

  Fragments arranged by size: 

                 643     320     295     140
 
BclI T'GATC_A

Cuts at:      0     245    1398 
   Size:        245    1153
 
BglII A'GATC_T

Cuts at:      0    1185    1398 
   Size:       1185     213
 
BmgBI CAC'GTC

Cuts at:      0     251    1398 
   Size:        251    1147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     762    1398 
   Size:        762     636
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     686    1336    1398 
   Size:        479     207     650      62

  Fragments arranged by size: 

                 650     479     207      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     615    1398 
   Size:        615     783
 
BsaWI w'CCGG_w

Cuts at:      0     855    1398 
   Size:        855     543
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     125    1398 
   Size:        125    1273
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     834    1398 
   Size:        834     564
 
BsrFI r'CCGG_y

Cuts at:      0     136    1115    1398 
   Size:        136     979     283

  Fragments arranged by size: 

                 979     283     136
 
BsrGI T'GTAC_A

Cuts at:      0    1143    1398 
   Size:       1143     255
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1383    1398 
   Size:       1383      15
 
BstYI r'GATC_y

Cuts at:      0    1185    1398 
   Size:       1185     213
 
Bsu36I CC'TnA_GG

Cuts at:      0     182    1398 
   Size:        182    1216
 
BtsI GCAGTG_nn'

Cuts at:      0      56    1398 
   Size:         56    1342
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     308    1398 
   Size:        308    1090
 
EarI CTCTTCn'nnn_

Cuts at:      0     182     836    1176    1398 
   Size:        182     654     340     222

  Fragments arranged by size: 

                 654     340     222     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     479     536     686    1336    1398 
   Size:         89     390      57     150     650      62

  Fragments arranged by size: 

                 650     390     150      89      62      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     107    1398 
   Size:        107    1291
 
EcoRI G'AATT_C

Cuts at:      0     859    1000    1398 
   Size:        859     141     398

  Fragments arranged by size: 

                 859     398     141
 
EcoRV GAT'ATC

Cuts at:      0     290     675    1398 
   Size:        290     385     723

  Fragments arranged by size: 

                 723     385     290
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     431    1398 
   Size:        431     967
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     183     215    1340    1372    1398 
   Size:        183      32    1125      32      26

  Fragments arranged by size: 

                1125     183      32      32      26
 
HincII GTy'rAC

Cuts at:      0     254    1398 
   Size:        254    1144
 
HindIII A'AGCT_T

Cuts at:      0      20    1321    1398 
   Size:         20    1301      77

  Fragments arranged by size: 

                1301      77      20
 
NspI r_CATG'y

Cuts at:      0     358     790    1398 
   Size:        358     432     608

  Fragments arranged by size: 

                 608     432     358
 
PciI A'CATG_T

Cuts at:      0     354     786    1398 
   Size:        354     432     612

  Fragments arranged by size: 

                 612     432     354
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     709    1398 
   Size:        709     689
 
PfoI T'CCnGG_A

Cuts at:      0     495    1004    1313    1398 
   Size:        495     509     309      85

  Fragments arranged by size: 

                 509     495     309      85
 
SapI GCTCTTCn'nnn_

Cuts at:      0     836    1398 
   Size:        836     562
 
ScaI AGT'ACT

Cuts at:      0     570    1398 
   Size:        570     828
 
SfcI C'TryA_G

Cuts at:      0    1256    1398 
   Size:       1256     142
 
SmlI C'TyrA_G

Cuts at:      0     293    1398 
   Size:        293    1105
 
SpeI A'CTAG_T

Cuts at:      0     891    1398 
   Size:        891     507
 
SspI AAT'ATT

Cuts at:      0      79    1173    1398 
   Size:         79    1094     225

  Fragments arranged by size: 

                1094     225      79
 
StyI C'CwwG_G

Cuts at:      0     406    1398 
   Size:        406     992
 
TatI w'GTAC_w

Cuts at:      0     568    1143    1398 
   Size:        568     575     255

  Fragments arranged by size: 

                 575     568     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     491     523    1398 
   Size:        491      32     875

  Fragments arranged by size: 

                 875     491      32
 
XhoI C'TCGA_G

Cuts at:      0     293    1398 
   Size:        293    1105
 
XmnI GAAnn'nnTTC

Cuts at:      0    1343    1398 
   Size:       1343      55
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI     BanI     BbsI     BclI    BglII
    BmgBI     BmrI     BpmI     BsaI    BsaWI    BseRI    BspMI    BsrFI
    BsrGI   BstAPI    BstYI   Bsu36I     BtsI     DrdI     EarI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     NspI
     PciI    PflMI     PfoI     SapI     ScaI     SfcI     SmlI     SpeI
     SspI     StyI     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI EcoO109I     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XmaI     ZraI