Restriction map for
pF1KB5593
(Linear) (Six-base) MAPSORT of: KIBB5593.seq Check: 2874 from: 1 to: 5589
KIBB5593.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AatII G_ACGT'C
Cuts at: 0 529 5589
Size: 529 5060
AccI GT'mk_AC
Cuts at: 0 533 2937 3721 5589
Size: 533 2404 784 1868
Fragments arranged by size:
2404 1868 784 533
Acc65I G'GTAC_C
Cuts at: 0 2313 5589
Size: 2313 3276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1121 1259 2119 2252 4157 4534 4760
Size: 581 540 138 860 133 1905 377 226
Cuts at: 4760 5063 5265 5589
Size: 303 202 324
Fragments arranged by size:
1905 860 581 540 377 324 303 226
202 138 133
AflII C'TTAA_G
Cuts at: 0 1419 5589
Size: 1419 4170
AflIII A'CryG_T
Cuts at: 0 979 5589
Size: 979 4610
AleI CACnn'nnGTG
Cuts at: 0 3854 5589
Size: 3854 1735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 603 1877 5453 5589
Size: 603 1274 3576 136
Fragments arranged by size:
3576 1274 603 136
AlwNI CAG_nnn'CTG
Cuts at: 0 1071 4108 4828 4846 5077 5547 5589
Size: 1071 3037 720 18 231 470 42
Fragments arranged by size:
3037 1071 720 470 231 42 18
ApaI G_GGCC'C
Cuts at: 0 5312 5589
Size: 5312 277
ApoI r'AATT_y
Cuts at: 0 133 679 907 2158 3774 4981 5589
Size: 133 546 228 1251 1616 1207 608
Fragments arranged by size:
1616 1251 1207 608 546 228 133
AseI AT'TA_AT
Cuts at: 0 44 3131 3173 5589
Size: 44 3087 42 2416
Fragments arranged by size:
3087 2416 44 42
AvaI C'yCGr_G
Cuts at: 0 5501 5589
Size: 5501 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5341 5374 5589
Size: 5341 33 215
Fragments arranged by size:
5341 215 33
BamHI G'GATC_C
Cuts at: 0 3939 5589
Size: 3939 1650
BanI G'GyrC_C
Cuts at: 0 2313 5487 5589
Size: 2313 3174 102
Fragments arranged by size:
3174 2313 102
BanII G_rGCy'C
Cuts at: 0 3166 4583 4752 5312 5589
Size: 3166 1417 169 560 277
Fragments arranged by size:
3166 1417 560 277 169
BbsI GAAGACnn'nnnn_
Cuts at: 0 1376 1835 2240 3283 3917 4145 4540 4748
Size: 1376 459 405 1043 634 228 395 208
Cuts at: 4748 5589
Size: 841
Fragments arranged by size:
1376 1043 841 634 459 405 395 228
208
BbvCI CC'TCA_GC
Cuts at: 0 1831 5589
Size: 1831 3758
BciVI GTATCCnnnnn_n'
Cuts at: 0 503 2501 4185 5589
Size: 503 1998 1684 1404
Fragments arranged by size:
1998 1684 1404 503
BclI T'GATC_A
Cuts at: 0 89 5589
Size: 89 5500
BfrBI ATG'CAT
Cuts at: 0 3424 5589
Size: 3424 2165
BglI GCCn_nnn'nGGC
Cuts at: 0 1980 5589
Size: 1980 3609
BglII A'GATC_T
Cuts at: 0 1020 2244 2284 4668 5276 5589
Size: 1020 1224 40 2384 608 313
Fragments arranged by size:
2384 1224 1020 608 313 40
BlpI GC'TnA_GC
Cuts at: 0 936 3556 5589
Size: 936 2620 2033
Fragments arranged by size:
2620 2033 936
Bme1580I G_kGCm'C
Cuts at: 0 5312 5589
Size: 5312 277
BmrI ACTGGGnnnn_n'
Cuts at: 0 716 5154 5589
Size: 716 4438 435
Fragments arranged by size:
4438 716 435
BmtI G_CTAG'C
Cuts at: 0 3881 5589
Size: 3881 1708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 2048 3150 4204 5589
Size: 103 1945 1102 1054 1385
Fragments arranged by size:
1945 1385 1102 1054 103
Bpu10I CC'TnA_GC
Cuts at: 0 1831 3596 4146 5589
Size: 1831 1765 550 1443
Fragments arranged by size:
1831 1765 1443 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 3105 4336 4556 5589
Size: 611 2494 1231 220 1033
Fragments arranged by size:
2494 1231 1033 611 220
BsaI GGTCTCn'nnnn_
Cuts at: 0 1512 5589
Size: 1512 4077
BsaHI Gr'CG_yC
Cuts at: 0 526 5589
Size: 526 5063
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1442 1472 2252 2282 5204 5234 5589
Size: 1442 30 780 30 2922 30 355
Fragments arranged by size:
2922 1442 780 355 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1848 3784 4591 4618 5225 5589
Size: 1848 1936 807 27 607 364
Fragments arranged by size:
1936 1848 807 607 364 27
BseYI C'CCAG_C
Cuts at: 0 1000 4867 5589
Size: 1000 3867 722
Fragments arranged by size:
3867 1000 722
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 2435 3918 5449 5589
Size: 776 1659 1483 1531 140
Fragments arranged by size:
1659 1531 1483 776 140
BsiHKAI G_wGCw'C
Cuts at: 0 3073 4583 5589
Size: 3073 1510 1006
Fragments arranged by size:
3073 1510 1006
BsmI GAATG_Cn'
Cuts at: 0 1032 1133 3246 5589
Size: 1032 101 2113 2343
Fragments arranged by size:
2343 2113 1032 101
BsmBI CGTCTCn'nnnn_
Cuts at: 0 682 4137 5589
Size: 682 3455 1452
Fragments arranged by size:
3455 1452 682
Bsp1286I G_dGCh'C
Cuts at: 0 3073 3166 4583 4752 5312 5589
Size: 3073 93 1417 169 560 277
Fragments arranged by size:
3073 1417 560 277 169 93
BspHI T'CATG_A
Cuts at: 0 1308 1656 5589
Size: 1308 348 3933
Fragments arranged by size:
3933 1308 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2028 4331 5589
Size: 2028 2303 1258
Fragments arranged by size:
2303 2028 1258
BsrDI GCAATG_nn'
Cuts at: 0 4886 5589
Size: 4886 703
BsrGI T'GTAC_A
Cuts at: 0 2757 4994 5589
Size: 2757 2237 595
Fragments arranged by size:
2757 2237 595
BssSI C'ACGA_G
Cuts at: 0 4763 4990 5589
Size: 4763 227 599
Fragments arranged by size:
4763 599 227
BstAPI GCAn_nnn'nTGC
Cuts at: 0 137 1071 4828 5589
Size: 137 934 3757 761
Fragments arranged by size:
3757 934 761 137
BstEII G'GTnAC_C
Cuts at: 0 3601 5154 5496 5589
Size: 3601 1553 342 93
Fragments arranged by size:
3601 1553 342 93
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 3997 5441 5470 5589
Size: 81 3916 1444 29 119
Fragments arranged by size:
3916 1444 119 81 29
BstYI r'GATC_y
Cuts at: 0 547 1020 2244 2284 3939 4668 5276 5570
Size: 547 473 1224 40 1655 729 608 294
Cuts at: 5570 5589
Size: 19
Fragments arranged by size:
1655 1224 729 608 547 473 294 40
19
BstZ17I GTA'TAC
Cuts at: 0 2938 5589
Size: 2938 2651
Bsu36I CC'TnA_GG
Cuts at: 0 1541 3272 5589
Size: 1541 1731 2317
Fragments arranged by size:
2317 1731 1541
BtgI C'CryG_G
Cuts at: 0 4952 5589
Size: 4952 637
BtsI GCAGTG_nn'
Cuts at: 0 597 1944 2774 3906 5589
Size: 597 1347 830 1132 1683
Fragments arranged by size:
1683 1347 1132 830 597
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 109 144 4619 4654 5589
Size: 109 35 4475 35 935
Fragments arranged by size:
4475 935 109 35 35
DraI TTT'AAA
Cuts at: 0 3108 5589
Size: 3108 2481
DraIII CAC_nnn'GTG
Cuts at: 0 113 5589
Size: 113 5476
EaeI y'GGCC_r
Cuts at: 0 1295 5589
Size: 1295 4294
EarI CTCTTCn'nnn_
Cuts at: 0 20 245 1937 2032 2189 2630 3603 3632
Size: 20 225 1692 95 157 441 973 29
Cuts at: 3632 3656 4064 4311 4613 4714 5039 5589
Size: 24 408 247 302 101 325 550
Fragments arranged by size:
1692 973 550 441 408 325 302 247
225 157 101 95 29 24 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 5589
Size: 1008 4581
EcoICRI GAG'CTC
Cuts at: 0 4581 5589
Size: 4581 1008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 581 1121 1259 2048 2119 2252 3150
Size: 103 478 540 138 789 71 133 898
Cuts at: 3150 4157 4204 4534 4760 5063 5265 5589
Size: 1007 47 330 226 303 202 324
Fragments arranged by size:
1007 898 789 540 478 330 324 303
226 202 138 133 103 71 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1526 3856 5589
Size: 1526 2330 1733
Fragments arranged by size:
2330 1733 1526
EcoO109I rG'GnC_Cy
Cuts at: 0 169 2669 4320 5309 5589
Size: 169 2500 1651 989 280
Fragments arranged by size:
2500 1651 989 280 169
EcoRI G'AATT_C
Cuts at: 0 907 5589
Size: 907 4682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3571 3813 4322 5589
Size: 3571 242 509 1267
Fragments arranged by size:
3571 1267 509 242
HaeII r_GCGC'y
Cuts at: 0 2598 5589
Size: 2598 2991
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 501 533 1189 1195 1221 1227
Size: 222 32 247 32 656 6 26 6
Cuts at: 1227 3631 3663 3739 3771 4711 4743 5204 5236
Size: 2404 32 76 32 940 32 461 32
Cuts at: 5236 5371 5403 5589
Size: 135 32 186
Fragments arranged by size:
2404 940 656 461 247 222 186 135
76 32 32 32 32 32 32 32
26 6 6
HincII GTy'rAC
Cuts at: 0 241 2105 2511 4938 5589
Size: 241 1864 406 2427 651
Fragments arranged by size:
2427 1864 651 406 241
HpaI GTT'AAC
Cuts at: 0 2105 2511 5589
Size: 2105 406 3078
Fragments arranged by size:
3078 2105 406
KpnI G_GTAC'C
Cuts at: 0 2317 5589
Size: 2317 3272
MfeI C'AATT_G
Cuts at: 0 4810 5417 5468 5589
Size: 4810 607 51 121
Fragments arranged by size:
4810 607 121 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 3702 5495 5589
Size: 486 3216 1793 94
Fragments arranged by size:
3216 1793 486 94
MscI TGG'CCA
Cuts at: 0 1297 5589
Size: 1297 4292
MslI CAynn'nnrTG
Cuts at: 0 1859 2536 3854 4110 4957 5320 5589
Size: 1859 677 1318 256 847 363 269
Fragments arranged by size:
1859 1318 847 677 363 269 256
MspA1I CmG'CkG
Cuts at: 0 507 760 4614 4843 5355 5589
Size: 507 253 3854 229 512 234
Fragments arranged by size:
3854 512 507 253 234 229
NcoI C'CATG_G
Cuts at: 0 4952 5589
Size: 4952 637
NheI G'CTAG_C
Cuts at: 0 3877 5589
Size: 3877 1712
NsiI A_TGCA'T
Cuts at: 0 3426 5589
Size: 3426 2163
NspI r_CATG'y
Cuts at: 0 144 811 983 4530 5325 5589
Size: 144 667 172 3547 795 264
Fragments arranged by size:
3547 795 667 264 172 144
PciI A'CATG_T
Cuts at: 0 979 5589
Size: 979 4610
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 5547 5589
Size: 381 5166 42
Fragments arranged by size:
5166 381 42
PfoI T'CCnGG_A
Cuts at: 0 2622 3294 5589
Size: 2622 672 2295
Fragments arranged by size:
2622 2295 672
PpuMI rG'GwC_Cy
Cuts at: 0 2669 4320 5589
Size: 2669 1651 1269
Fragments arranged by size:
2669 1651 1269
PshAI GACnn'nnGTC
Cuts at: 0 529 5589
Size: 529 5060
PspOMI G'GGCC_C
Cuts at: 0 5308 5589
Size: 5308 281
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1226 1258 5589
Size: 1226 32 4331
Fragments arranged by size:
4331 1226 32
PstI C_TGCA'G
Cuts at: 0 2781 3811 4443 5589
Size: 2781 1030 632 1146
Fragments arranged by size:
2781 1146 1030 632
PvuII CAG'CTG
Cuts at: 0 760 4614 4843 5355 5589
Size: 760 3854 229 512 234
Fragments arranged by size:
3854 760 512 234 229
SacI G_AGCT'C
Cuts at: 0 4583 5589
Size: 4583 1006
SapI GCTCTTCn'nnn_
Cuts at: 0 20 245 3656 4064 5589
Size: 20 225 3411 408 1525
Fragments arranged by size:
3411 1525 408 225 20
ScaI AGT'ACT
Cuts at: 0 3150 5589
Size: 3150 2439
SexAI A'CCwGG_T
Cuts at: 0 2115 2316 3815 5589
Size: 2115 201 1499 1774
Fragments arranged by size:
2115 1774 1499 201
SfcI C'TryA_G
Cuts at: 0 387 412 2288 2777 3807 4439 5589
Size: 387 25 1876 489 1030 632 1150
Fragments arranged by size:
1876 1150 1030 632 489 387 25
SmlI C'TyrA_G
Cuts at: 0 626 1419 3120 4315 4571 5589
Size: 626 793 1701 1195 256 1018
Fragments arranged by size:
1701 1195 1018 793 626 256
SpeI A'CTAG_T
Cuts at: 0 1872 3487 5589
Size: 1872 1615 2102
Fragments arranged by size:
2102 1872 1615
SphI G_CATG'C
Cuts at: 0 144 5589
Size: 144 5445
SspI AAT'ATT
Cuts at: 0 1264 1365 1379 1641 3129 3824 5107 5589
Size: 1264 101 14 262 1488 695 1283 482
Fragments arranged by size:
1488 1283 1264 695 482 262 101 14
StuI AGG'CCT
Cuts at: 0 1532 4412 5589
Size: 1532 2880 1177
Fragments arranged by size:
2880 1532 1177
StyI C'CwwG_G
Cuts at: 0 474 1111 2432 4660 4952 5238 5397 5589
Size: 474 637 1321 2228 292 286 159 192
Fragments arranged by size:
2228 1321 637 474 292 286 192 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3595 3949 4100 5142 5429 5499 5589
Size: 3595 354 151 1042 287 70 90
Fragments arranged by size:
3595 1042 354 287 151 90 70
TatI w'GTAC_w
Cuts at: 0 30 1906 1961 2757 3061 3148 4994 5589
Size: 30 1876 55 796 304 87 1846 595
Fragments arranged by size:
1876 1846 796 595 304 87 55 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2653 2685 3924 3956 5589
Size: 2653 32 1239 32 1633
Fragments arranged by size:
2653 1633 1239 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5160 5589
Size: 5160 429
XbaI T'CTAG_A
Cuts at: 0 4671 5589
Size: 4671 918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5334 5589
Size: 5334 255
XmnI GAAnn'nnTTC
Cuts at: 0 1106 1337 2578 3200 3347 3665 5589
Size: 1106 231 1241 622 147 318 1924
Fragments arranged by size:
1924 1241 1106 622 318 231 147
ZraI GAC'GTC
Cuts at: 0 527 5589
Size: 527 5062
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AleI AlfI
AlwNI ApaI ApoI AseI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HpaI KpnI MfeI
MmeI MscI MslI MspA1I NcoI NheI NsiI NspI
PciI PflMI PfoI PpuMI PshAI PspOMI PsrI PstI
PvuII SacI SapI ScaI SexAI SfcI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AhdI AloI ApaLI AscI
AsiSI AvrII BbeI BcgI BmgBI BplI BsaAI BsaBI
BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstBI
BtgZI ClaI DrdI EagI EcoRV FseI FspI FspAI
HindIII KasI MluI NaeI NarI NdeI NgoMIV NotI
NruI PacI PasI PmeI PmlI PpiI PsiI PspXI
PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI XmaI