Restriction map for
pF1KB5590
(Linear) (Six-base) MAPSORT of: KIBB5590.seq Check: 5260 from: 1 to: 1047
KIBB5590.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AflIII A'CryG_T
Cuts at: 0 557 1047
Size: 557 490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 609 641 1047
Size: 609 32 406
Fragments arranged by size:
609 406 32
ApaI G_GGCC'C
Cuts at: 0 22 1047
Size: 22 1025
ApaLI G'TGCA_C
Cuts at: 0 201 282 369 1047
Size: 201 81 87 678
Fragments arranged by size:
678 201 87 81
ApoI r'AATT_y
Cuts at: 0 844 1047
Size: 844 203
AvaI C'yCGr_G
Cuts at: 0 450 469 656 1047
Size: 450 19 187 391
Fragments arranged by size:
450 391 187 19
BanI G'GyrC_C
Cuts at: 0 101 117 1047
Size: 101 16 930
Fragments arranged by size:
930 101 16
BanII G_rGCy'C
Cuts at: 0 22 506 1047
Size: 22 484 541
Fragments arranged by size:
541 484 22
BbeI G_GCGC'C
Cuts at: 0 121 1047
Size: 121 926
BbsI GAAGACnn'nnnn_
Cuts at: 0 560 835 1047
Size: 560 275 212
Fragments arranged by size:
560 275 212
BglI GCCn_nnn'nGGC
Cuts at: 0 134 538 926 1047
Size: 134 404 388 121
Fragments arranged by size:
404 388 134 121
Bme1580I G_kGCm'C
Cuts at: 0 22 106 205 286 373 922 1047
Size: 22 84 99 81 87 549 125
Fragments arranged by size:
549 125 99 87 84 81 22
BsaAI yAC'GTr
Cuts at: 0 374 1047
Size: 374 673
BsaBI GATnn'nnATC
Cuts at: 0 646 955 1047
Size: 646 309 92
Fragments arranged by size:
646 309 92
BsaHI Gr'CG_yC
Cuts at: 0 118 1047
Size: 118 929
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 478 508 609 639 661 691
Size: 238 30 210 30 101 30 22 30
Cuts at: 691 901 931 1047
Size: 210 30 116
Fragments arranged by size:
238 210 210 116 101 30 30 30
30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 468 499 1047
Size: 37 431 31 548
Fragments arranged by size:
548 431 37 31
BseYI C'CCAG_C
Cuts at: 0 189 430 1047
Size: 189 241 617
Fragments arranged by size:
617 241 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 801 861 1047
Size: 429 372 60 186
Fragments arranged by size:
429 372 186 60
BsiEI CG_ry'CG
Cuts at: 0 67 71 870 1047
Size: 67 4 799 177
Fragments arranged by size:
799 177 67 4
BsiHKAI G_wGCw'C
Cuts at: 0 205 286 303 373 1047
Size: 205 81 17 70 674
Fragments arranged by size:
674 205 81 70 17
Bsp1286I G_dGCh'C
Cuts at: 0 22 106 205 286 303 373 506 922
Size: 22 84 99 81 17 70 133 416
Cuts at: 922 1047
Size: 125
Fragments arranged by size:
416 133 125 99 84 81 70 22
17
BspHI T'CATG_A
Cuts at: 0 935 1047
Size: 935 112
BsrFI r'CCGG_y
Cuts at: 0 62 66 98 114 903 1047
Size: 62 4 32 16 789 144
Fragments arranged by size:
789 144 62 32 16 4
BssSI C'ACGA_G
Cuts at: 0 145 1047
Size: 145 902
BstAPI GCAn_nnn'nTGC
Cuts at: 0 764 1047
Size: 764 283
BstEII G'GTnAC_C
Cuts at: 0 660 1047
Size: 660 387
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 597 1047
Size: 189 408 450
Fragments arranged by size:
450 408 189
BtgI C'CryG_G
Cuts at: 0 518 1047
Size: 518 529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 618 653 1047
Size: 618 35 394
Fragments arranged by size:
618 394 35
DraIII CAC_nnn'GTG
Cuts at: 0 614 1047
Size: 614 433
DrdI GACnn_nn'nnGTC
Cuts at: 0 931 1047
Size: 931 116
EaeI y'GGCC_r
Cuts at: 0 64 68 75 260 867 1047
Size: 64 4 7 185 607 180
Fragments arranged by size:
607 185 180 64 7 4
EagI C'GGCC_G
Cuts at: 0 64 68 867 1047
Size: 64 4 799 180
Fragments arranged by size:
799 180 64 4
EarI CTCTTCn'nnn_
Cuts at: 0 244 734 1047
Size: 244 490 313
Fragments arranged by size:
490 313 244
EcoNI CCTnn'n_nnAGG
Cuts at: 0 450 681 1047
Size: 450 231 366
Fragments arranged by size:
450 366 231
EcoO109I rG'GnC_Cy
Cuts at: 0 19 234 1047
Size: 19 215 813
Fragments arranged by size:
813 215 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 1047
Size: 255 792
FseI GG_CCGG'CC
Cuts at: 0 70 1047
Size: 70 977
HaeII r_GCGC'y
Cuts at: 0 121 623 1047
Size: 121 502 424
Fragments arranged by size:
502 424 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 661 693 1047
Size: 238 32 391 32 354
Fragments arranged by size:
391 354 238 32 32
KasI G'GCGC_C
Cuts at: 0 117 1047
Size: 117 930
MslI CAynn'nnrTG
Cuts at: 0 823 1047
Size: 823 224
MspA1I CmG'CkG
Cuts at: 0 89 189 335 356 1047
Size: 89 100 146 21 691
Fragments arranged by size:
691 146 100 89 21
NaeI GCC'GGC
Cuts at: 0 64 68 116 1047
Size: 64 4 48 931
Fragments arranged by size:
931 64 48 4
NarI GG'CG_CC
Cuts at: 0 118 1047
Size: 118 929
NgoMIV G'CCGG_C
Cuts at: 0 62 66 114 1047
Size: 62 4 48 933
Fragments arranged by size:
933 62 48 4
NspI r_CATG'y
Cuts at: 0 402 1047
Size: 402 645
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 614 1047
Size: 519 95 433
Fragments arranged by size:
519 433 95
PfoI T'CCnGG_A
Cuts at: 0 987 1047
Size: 987 60
PmlI CAC'GTG
Cuts at: 0 374 1047
Size: 374 673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 609 641 1047
Size: 609 32 406
Fragments arranged by size:
609 406 32
PshAI GACnn'nnGTC
Cuts at: 0 422 1047
Size: 422 625
PspOMI G'GGCC_C
Cuts at: 0 18 1047
Size: 18 1029
PstI C_TGCA'G
Cuts at: 0 61 88 685 1047
Size: 61 27 597 362
Fragments arranged by size:
597 362 61 27
PvuII CAG'CTG
Cuts at: 0 189 356 1047
Size: 189 167 691
Fragments arranged by size:
691 189 167
SbfI CC_TGCA'GG
Cuts at: 0 685 1047
Size: 685 362
SfcI C'TryA_G
Cuts at: 0 57 84 681 1020 1047
Size: 57 27 597 339 27
Fragments arranged by size:
597 339 57 27 27
SfoI GGC'GCC
Cuts at: 0 119 1047
Size: 119 928
SmaI CCC'GGG
Cuts at: 0 471 658 1047
Size: 471 187 389
Fragments arranged by size:
471 389 187
SphI G_CATG'C
Cuts at: 0 402 1047
Size: 402 645
StuI AGG'CCT
Cuts at: 0 720 1047
Size: 720 327
StyI C'CwwG_G
Cuts at: 0 506 1047
Size: 506 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 480 1047
Size: 480 567
TatI w'GTAC_w
Cuts at: 0 1016 1047
Size: 1016 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 901 933 1047
Size: 478 32 391 32 114
Fragments arranged by size:
478 391 114 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 520 903 1047
Size: 520 383 144
Fragments arranged by size:
520 383 144
XmaI C'CCGG_G
Cuts at: 0 469 656 1047
Size: 469 187 391
Fragments arranged by size:
469 391 187
Enzymes that do cut:
AflIII AloI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BglI Bme1580I BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrFI
BssSI BstAPI BstEII BstXI BtgI CspCI DraIII DrdI
EaeI EagI EarI EcoNI EcoO109I FalI FseI HaeII
Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI
PflMI PfoI PmlI PpiI PshAI PspOMI PstI PvuII
SbfI SfcI SfoI SmaI SphI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI EciI EcoICRI Eco57MI EcoRI
EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI