(Linear) (Six-base) MAPSORT of: KIBB5576.seq  Check: 1521  from: 1  to: 492



KIBB5576.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     303     492 
   Size:        303     189
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     492 
   Size:        401      91
 
ApaI G_GGCC'C

Cuts at:      0     245     492 
   Size:        245     247
 
BanI G'GyrC_C

Cuts at:      0     286     492 
   Size:        286     206
 
BanII G_rGCy'C

Cuts at:      0     245     492 
   Size:        245     247
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     329     492 
   Size:        329     163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      85     119     492 
   Size:         85      34     373

  Fragments arranged by size: 

                 373      85      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      65     492 
   Size:         65     427
 
Bme1580I G_kGCm'C

Cuts at:      0     245     492 
   Size:        245     247
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      64     492 
   Size:         64     428
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     422     492 
   Size:        196     226      70

  Fragments arranged by size: 

                 226     196      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     366     492 
   Size:        366     126
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     284     492 
   Size:        284     208
 
BseYI C'CCAG_C

Cuts at:      0      13     492 
   Size:         13     479
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      84     492 
   Size:         84     408
 
Bsp1286I G_dGCh'C

Cuts at:      0     245     492 
   Size:        245     247
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     303     492 
   Size:        303     189
 
BsrFI r'CCGG_y

Cuts at:      0       9     464     492 
   Size:          9     455      28

  Fragments arranged by size: 

                 455      28       9
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      50     492 
   Size:         50     442
 
BtgI C'CryG_G

Cuts at:      0     236     492 
   Size:        236     256
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     156     492 
   Size:        156     336
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     492 
   Size:         67     425
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     401     422     492 
   Size:        196     205      21      70

  Fragments arranged by size: 

                 205     196      70      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     365     492 
   Size:        365     127
 
EcoO109I rG'GnC_Cy

Cuts at:      0     207     241     242     386     492 
   Size:        207      34       1     144     106

  Fragments arranged by size: 

                 207     144     106      34       1
 
MspA1I CmG'CkG

Cuts at:      0      87     492 
   Size:         87     405
 
NaeI GCC'GGC

Cuts at:      0     466     492 
   Size:        466      26
 
NgoMIV G'CCGG_C

Cuts at:      0     464     492 
   Size:        464      28
 
PfoI T'CCnGG_A

Cuts at:      0     125     492 
   Size:        125     367
 
PpuMI rG'GwC_Cy

Cuts at:      0     386     492 
   Size:        386     106
 
PspOMI G'GGCC_C

Cuts at:      0     241     492 
   Size:        241     251
 
PstI C_TGCA'G

Cuts at:      0     308     492 
   Size:        308     184
 
PvuII CAG'CTG

Cuts at:      0      87     492 
   Size:         87     405
 
SanDI GG'GwC_CC

Cuts at:      0     386     492 
   Size:        386     106
 
SfcI C'TryA_G

Cuts at:      0     304     492 
   Size:        304     188
 
StuI AGG'CCT

Cuts at:      0      49     492 
   Size:         49     443
 
StyI C'CwwG_G

Cuts at:      0     210     492 
   Size:        210     282
 
Tth111I GACn'n_nGTC

Cuts at:      0     157     492 
   Size:        157     335
 

 Enzymes that do cut:

     AarI     AcuI     ApaI     BanI    BanII     BbsI     BcgI     BglI
 Bme1580I     BmrI     BpmI   Bpu10I    BseRI    BseYI    BsmBI Bsp1286I
    BspMI    BsrFI   BstAPI     BtgI    BtgZI     EarI  Eco57MI    EcoNI
 EcoO109I   MspA1I     NaeI   NgoMIV     PfoI    PpuMI   PspOMI     PstI
    PvuII    SanDI     SfcI     StuI     StyI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI