Restriction map for
pF1KB5574
(Linear) (Six-base) MAPSORT of: KIBB5574.seq Check: 2133 from: 1 to: 636
KIBB5574.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 251 636
Size: 251 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 636
Size: 622 14
AlwNI CAG_nnn'CTG
Cuts at: 0 547 636
Size: 547 89
ApaLI G'TGCA_C
Cuts at: 0 115 636
Size: 115 521
AvaI C'yCGr_G
Cuts at: 0 157 177 636
Size: 157 20 459
Fragments arranged by size:
459 157 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 636
Size: 18 618
BbvCI CC'TCA_GC
Cuts at: 0 191 636
Size: 191 445
Bme1580I G_kGCm'C
Cuts at: 0 119 636
Size: 119 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 250 636
Size: 168 82 386
Fragments arranged by size:
386 168 82
Bpu10I CC'TnA_GC
Cuts at: 0 191 636
Size: 191 445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 636
Size: 508 128
BsaAI yAC'GTr
Cuts at: 0 114 356 636
Size: 114 242 280
Fragments arranged by size:
280 242 114
BsaHI Gr'CG_yC
Cuts at: 0 47 183 636
Size: 47 136 453
Fragments arranged by size:
453 136 47
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 9 636
Size: 9 627
BseYI C'CCAG_C
Cuts at: 0 99 244 422 636
Size: 99 145 178 214
Fragments arranged by size:
214 178 145 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 560 636
Size: 259 301 76
Fragments arranged by size:
301 259 76
BsiEI CG_ry'CG
Cuts at: 0 594 636
Size: 594 42
BsiHKAI G_wGCw'C
Cuts at: 0 119 636
Size: 119 517
BsmBI CGTCTCn'nnnn_
Cuts at: 0 492 636
Size: 492 144
Bsp1286I G_dGCh'C
Cuts at: 0 119 636
Size: 119 517
BsrBI CCG'CTC
Cuts at: 0 59 557 636
Size: 59 498 79
Fragments arranged by size:
498 79 59
BsrFI r'CCGG_y
Cuts at: 0 136 171 636
Size: 136 35 465
Fragments arranged by size:
465 136 35
BssHII G'CGCG_C
Cuts at: 0 132 152 636
Size: 132 20 484
Fragments arranged by size:
484 132 20
BssSI C'ACGA_G
Cuts at: 0 445 636
Size: 445 191
BstEII G'GTnAC_C
Cuts at: 0 303 636
Size: 303 333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 636
Size: 99 537
BtgI C'CryG_G
Cuts at: 0 323 389 401 431 636
Size: 323 66 12 30 205
Fragments arranged by size:
323 205 66 30 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 236 636
Size: 236 400
EaeI y'GGCC_r
Cuts at: 0 320 330 386 398 591 636
Size: 320 10 56 12 193 45
Fragments arranged by size:
320 193 56 45 12 10
EagI C'GGCC_G
Cuts at: 0 591 636
Size: 591 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 521 636
Size: 521 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 250 622 636
Size: 168 82 372 14
Fragments arranged by size:
372 168 82 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 636
Size: 190 446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 627 636
Size: 627 9
FspI TGC'GCA
Cuts at: 0 472 636
Size: 472 164
HaeII r_GCGC'y
Cuts at: 0 18 100 636
Size: 18 82 536
Fragments arranged by size:
536 82 18
MscI TGG'CCA
Cuts at: 0 332 636
Size: 332 304
MspA1I CmG'CkG
Cuts at: 0 273 391 636
Size: 273 118 245
Fragments arranged by size:
273 245 118
NaeI GCC'GGC
Cuts at: 0 138 636
Size: 138 498
NcoI C'CATG_G
Cuts at: 0 431 636
Size: 431 205
NgoMIV G'CCGG_C
Cuts at: 0 136 636
Size: 136 500
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 636
Size: 567 69
PfoI T'CCnGG_A
Cuts at: 0 145 636
Size: 145 491
PmlI CAC'GTG
Cuts at: 0 356 636
Size: 356 280
PshAI GACnn'nnGTC
Cuts at: 0 517 636
Size: 517 119
PstI C_TGCA'G
Cuts at: 0 290 348 586 636
Size: 290 58 238 50
Fragments arranged by size:
290 238 58 50
PvuII CAG'CTG
Cuts at: 0 273 636
Size: 273 363
SacII CC_GC'GG
Cuts at: 0 392 636
Size: 392 244
SfcI C'TryA_G
Cuts at: 0 286 344 582 636
Size: 286 58 238 54
Fragments arranged by size:
286 238 58 54
SmaI CCC'GGG
Cuts at: 0 179 636
Size: 179 457
SmlI C'TyrA_G
Cuts at: 0 523 636
Size: 523 113
StyI C'CwwG_G
Cuts at: 0 92 431 636
Size: 92 339 205
Fragments arranged by size:
339 205 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 322 636
Size: 12 310 314
Fragments arranged by size:
314 310 12
XmaI C'CCGG_G
Cuts at: 0 177 636
Size: 177 459
Enzymes that do cut:
AccI AcuI AlwNI ApaLI AvaI BbsI BbvCI Bme1580I
BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI
BstEII BstXI BtgI BtgZI EaeI EagI EciI Eco57MI
EcoNI FalI FspI HaeII MscI MspA1I NaeI NcoI
NgoMIV PflMI PfoI PmlI PshAI PstI PvuII SacII
SfcI SmaI SmlI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoO109I EcoRI EcoRV FseI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI