Restriction map for
pF1KB5573
(Linear) (Six-base) MAPSORT of: KIBB5573.seq Check: 9620 from: 1 to: 3615
KIBB5573.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 222 1578 3615
Size: 222 1356 2037
Fragments arranged by size:
2037 1356 222
AccI GT'mk_AC
Cuts at: 0 168 3615
Size: 168 3447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1657 2398 2423 3039 3181 3190 3615
Size: 715 942 741 25 616 142 9 425
Fragments arranged by size:
942 741 715 616 425 142 25 9
AhdI GACnn_n'nnGTC
Cuts at: 0 2503 3615
Size: 2503 1112
AleI CACnn'nnGTG
Cuts at: 0 2290 2899 3615
Size: 2290 609 716
Fragments arranged by size:
2290 716 609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1367 3580 3615
Size: 1367 2213 35
Fragments arranged by size:
2213 1367 35
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 3615
Size: 550 32 3033
Fragments arranged by size:
3033 550 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3087 3615
Size: 3087 528
ApoI r'AATT_y
Cuts at: 0 2176 2818 3037 3403 3615
Size: 2176 642 219 366 212
Fragments arranged by size:
2176 642 366 219 212
AseI AT'TA_AT
Cuts at: 0 131 3615
Size: 131 3484
AvaI C'yCGr_G
Cuts at: 0 1672 2108 3615
Size: 1672 436 1507
Fragments arranged by size:
1672 1507 436
BanI G'GyrC_C
Cuts at: 0 1029 1774 2031 2705 2847 3187 3615
Size: 1029 745 257 674 142 340 428
Fragments arranged by size:
1029 745 674 428 340 257 142
BanII G_rGCy'C
Cuts at: 0 712 1886 1949 2681 3615
Size: 712 1174 63 732 934
Fragments arranged by size:
1174 934 732 712 63
BbeI G_GCGC'C
Cuts at: 0 1778 2709 3615
Size: 1778 931 906
Fragments arranged by size:
1778 931 906
BbsI GAAGACnn'nnnn_
Cuts at: 0 1476 3615
Size: 1476 2139
BbvCI CC'TCA_GC
Cuts at: 0 3070 3615
Size: 3070 545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1860 1894 3615
Size: 1860 34 1721
Fragments arranged by size:
1860 1721 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3307 3615
Size: 3307 308
BclI T'GATC_A
Cuts at: 0 476 2639 3615
Size: 476 2163 976
Fragments arranged by size:
2163 976 476
BfrBI ATG'CAT
Cuts at: 0 2770 3615
Size: 2770 845
BglI GCCn_nnn'nGGC
Cuts at: 0 1269 2714 3615
Size: 1269 1445 901
Fragments arranged by size:
1445 1269 901
BglII A'GATC_T
Cuts at: 0 213 1632 2234 3615
Size: 213 1419 602 1381
Fragments arranged by size:
1419 1381 602 213
Bme1580I G_kGCm'C
Cuts at: 0 1673 3010 3615
Size: 1673 1337 605
Fragments arranged by size:
1673 1337 605
BmrI ACTGGGnnnn_n'
Cuts at: 0 3570 3615
Size: 3570 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 346 1544 2600 3615
Size: 238 108 1198 1056 1015
Fragments arranged by size:
1198 1056 1015 238 108
Bpu10I CC'TnA_GC
Cuts at: 0 2243 3070 3615
Size: 2243 827 545
Fragments arranged by size:
2243 827 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 1354 3615
Size: 1354 2261
BsaBI GATnn'nnATC
Cuts at: 0 3028 3615
Size: 3028 587
BsaHI Gr'CG_yC
Cuts at: 0 1775 2706 3615
Size: 1775 931 909
Fragments arranged by size:
1775 931 909
BsaWI w'CCGG_w
Cuts at: 0 1817 1904 3615
Size: 1817 87 1711
Fragments arranged by size:
1817 1711 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1166 1196 2503 2533 3615
Size: 550 30 586 30 1307 30 1082
Fragments arranged by size:
1307 1082 586 550 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1690 3214 3430 3433 3469 3615
Size: 1690 1524 216 3 36 146
Fragments arranged by size:
1690 1524 216 146 36 3
BseYI C'CCAG_C
Cuts at: 0 2866 3178 3615
Size: 2866 312 437
Fragments arranged by size:
2866 437 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 2638 3586 3615
Size: 54 2584 948 29
Fragments arranged by size:
2584 948 54 29
BsiHKAI G_wGCw'C
Cuts at: 0 712 1614 1729 3500 3615
Size: 712 902 115 1771 115
Fragments arranged by size:
1771 902 712 115 115
BsmI GAATG_Cn'
Cuts at: 0 2132 2770 3350 3615
Size: 2132 638 580 265
Fragments arranged by size:
2132 638 580 265
Bsp1286I G_dGCh'C
Cuts at: 0 712 1614 1673 1729 1886 1949 2681 3010
Size: 712 902 59 56 157 63 732 329
Cuts at: 3010 3500 3615
Size: 490 115
Fragments arranged by size:
902 732 712 490 329 157 115 63
59 56
BspEI T'CCGG_A
Cuts at: 0 1904 3615
Size: 1904 1711
BspHI T'CATG_A
Cuts at: 0 3389 3615
Size: 3389 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 222 1578 2322 3615
Size: 222 1356 744 1293
Fragments arranged by size:
1356 1293 744 222
BsrDI GCAATG_nn'
Cuts at: 0 2114 3615
Size: 2114 1501
BsrFI r'CCGG_y
Cuts at: 0 1096 2183 2327 3615
Size: 1096 1087 144 1288
Fragments arranged by size:
1288 1096 1087 144
BsrGI T'GTAC_A
Cuts at: 0 150 1694 1763 3061 3615
Size: 150 1544 69 1298 554
Fragments arranged by size:
1544 1298 554 150 69
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 1296 1500 3615
Size: 39 1257 204 2115
Fragments arranged by size:
2115 1257 204 39
BstYI r'GATC_y
Cuts at: 0 213 1632 1970 2234 2939 3269 3615
Size: 213 1419 338 264 705 330 346
Fragments arranged by size:
1419 705 346 338 330 264 213
BtgI C'CryG_G
Cuts at: 0 1177 1364 1886 2402 2943 3615
Size: 1177 187 522 516 541 672
Fragments arranged by size:
1177 672 541 522 516 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2394 3615
Size: 2394 1221
BtsI GCAGTG_nn'
Cuts at: 0 547 1838 2492 3615
Size: 547 1291 654 1123
Fragments arranged by size:
1291 1123 654 547
DraIII CAC_nnn'GTG
Cuts at: 0 249 3615
Size: 249 3366
DrdI GACnn_nn'nnGTC
Cuts at: 0 1510 3615
Size: 1510 2105
EaeI y'GGCC_r
Cuts at: 0 41 798 2558 2669 2715 3615
Size: 41 757 1760 111 46 900
Fragments arranged by size:
1760 900 757 111 46 41
EarI CTCTTCn'nnn_
Cuts at: 0 305 2920 3103 3446 3615
Size: 305 2615 183 343 169
Fragments arranged by size:
2615 343 305 183 169
EcoICRI GAG'CTC
Cuts at: 0 710 3615
Size: 710 2905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 346 715 1544 1657 2398 2423 2600
Size: 238 108 369 829 113 741 25 177
Cuts at: 2600 3039 3181 3190 3615
Size: 439 142 9 425
Fragments arranged by size:
829 741 439 425 369 238 177 142
113 108 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 188 3172 3445 3615
Size: 188 2984 273 170
Fragments arranged by size:
2984 273 188 170
EcoO109I rG'GnC_Cy
Cuts at: 0 776 2013 2253 3285 3615
Size: 776 1237 240 1032 330
Fragments arranged by size:
1237 1032 776 330 240
EcoRI G'AATT_C
Cuts at: 0 2176 3403 3615
Size: 2176 1227 212
Fragments arranged by size:
2176 1227 212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 2775 2976 3615
Size: 294 2481 201 639
Fragments arranged by size:
2481 639 294 201
FspI TGC'GCA
Cuts at: 0 1714 1795 3615
Size: 1714 81 1820
Fragments arranged by size:
1820 1714 81
HaeII r_GCGC'y
Cuts at: 0 1778 2709 3615
Size: 1778 931 906
Fragments arranged by size:
1778 931 906
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1166 1198 1542 1574 2489 2503
Size: 694 32 440 32 344 32 915 14
Cuts at: 2503 2521 2535 2573 2605 3254 3286 3615
Size: 18 14 38 32 649 32 329
Fragments arranged by size:
915 694 649 440 344 329 38 32
32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 0 589 828 1409 3615
Size: 589 239 581 2206
Fragments arranged by size:
2206 589 581 239
HindIII A'AGCT_T
Cuts at: 0 1436 2245 3615
Size: 1436 809 1370
Fragments arranged by size:
1436 1370 809
KasI G'GCGC_C
Cuts at: 0 1774 2705 3615
Size: 1774 931 910
Fragments arranged by size:
1774 931 910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3485 3560 3615
Size: 3485 75 55
Fragments arranged by size:
3485 75 55
MscI TGG'CCA
Cuts at: 0 2671 3615
Size: 2671 944
MslI CAynn'nnrTG
Cuts at: 0 76 484 1349 1426 2290 2746 2899 3615
Size: 76 408 865 77 864 456 153 716
Fragments arranged by size:
865 864 716 456 408 153 77 76
MspA1I CmG'CkG
Cuts at: 0 817 3615
Size: 817 2798
NarI GG'CG_CC
Cuts at: 0 1775 2706 3615
Size: 1775 931 909
Fragments arranged by size:
1775 931 909
NcoI C'CATG_G
Cuts at: 0 1177 1364 1886 2402 2943 3615
Size: 1177 187 522 516 541 672
Fragments arranged by size:
1177 672 541 522 516 187
NdeI CA'TA_TG
Cuts at: 0 3525 3615
Size: 3525 90
NsiI A_TGCA'T
Cuts at: 0 2772 3615
Size: 2772 843
PflMI CCAn_nnn'nTGG
Cuts at: 0 786 3615
Size: 786 2829
PfoI T'CCnGG_A
Cuts at: 0 323 1499 1811 3440 3615
Size: 323 1176 312 1629 175
Fragments arranged by size:
1629 1176 323 312 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 3615
Size: 550 32 3033
Fragments arranged by size:
3033 550 32
PpuMI rG'GwC_Cy
Cuts at: 0 776 2013 2253 3615
Size: 776 1237 240 1362
Fragments arranged by size:
1362 1237 776 240
PshAI GACnn'nnGTC
Cuts at: 0 781 3615
Size: 781 2834
PstI C_TGCA'G
Cuts at: 0 166 213 542 666 1058 2534 3615
Size: 166 47 329 124 392 1476 1081
Fragments arranged by size:
1476 1081 392 329 166 124 47
PvuII CAG'CTG
Cuts at: 0 817 3615
Size: 817 2798
SacI G_AGCT'C
Cuts at: 0 712 3615
Size: 712 2903
SanDI GG'GwC_CC
Cuts at: 0 776 3615
Size: 776 2839
SapI GCTCTTCn'nnn_
Cuts at: 0 305 2920 3615
Size: 305 2615 695
Fragments arranged by size:
2615 695 305
ScaI AGT'ACT
Cuts at: 0 1213 3615
Size: 1213 2402
SfcI C'TryA_G
Cuts at: 0 162 209 538 662 1054 2530 2996 3592
Size: 162 47 329 124 392 1476 466 596
Cuts at: 3592 3615
Size: 23
Fragments arranged by size:
1476 596 466 392 329 162 124 47
23
SfoI GGC'GCC
Cuts at: 0 1776 2707 3615
Size: 1776 931 908
Fragments arranged by size:
1776 931 908
SmaI CCC'GGG
Cuts at: 0 2110 3615
Size: 2110 1505
SrfI GCCC'GGGC
Cuts at: 0 2110 3615
Size: 2110 1505
StuI AGG'CCT
Cuts at: 0 1577 3615
Size: 1577 2038
StyI C'CwwG_G
Cuts at: 0 681 1177 1364 1886 2402 2943 3209 3615
Size: 681 496 187 522 516 541 266 406
Fragments arranged by size:
681 541 522 516 496 406 266 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1618 2880 3615
Size: 1618 1262 735
Fragments arranged by size:
1618 1262 735
TatI w'GTAC_w
Cuts at: 0 150 1192 1211 1245 1694 1763 3061 3615
Size: 150 1042 19 34 449 69 1298 554
Fragments arranged by size:
1298 1042 554 449 150 69 34 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 3615
Size: 233 32 3350
Fragments arranged by size:
3350 233 32
Tth111I GACn'n_nGTC
Cuts at: 0 19 733 3615
Size: 19 714 2882
Fragments arranged by size:
2882 714 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 3615
Size: 345 3270
XmaI C'CCGG_G
Cuts at: 0 2108 3615
Size: 2108 1507
XmnI GAAnn'nnTTC
Cuts at: 0 299 526 3350 3615
Size: 299 227 2824 265
Fragments arranged by size:
2824 299 265 227
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AloI AlwNI
ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI
PflMI PfoI PpiI PpuMI PshAI PstI PvuII SacI
SanDI SapI ScaI SfcI SfoI SmaI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BlpI BmgBI
BmtI BplI BpuEI BsaAI BsiEI BsiWI BsmBI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI EagI EciI EcoRV FseI FspAI HpaI
KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
SwaI XbaI XhoI ZraI