Restriction map for
pF1KB5569
(Linear) (Six-base) MAPSORT of: KIBB5569.seq Check: 2263 from: 1 to: 1407
KIBB5569.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 271 708 1407
Size: 151 120 437 699
Fragments arranged by size:
699 437 151 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1148 1407
Size: 1148 259
AlwNI CAG_nnn'CTG
Cuts at: 0 241 1407
Size: 241 1166
ApoI r'AATT_y
Cuts at: 0 205 997 1336 1358 1407
Size: 205 792 339 22 49
Fragments arranged by size:
792 339 205 49 22
BamHI G'GATC_C
Cuts at: 0 1221 1407
Size: 1221 186
BanII G_rGCy'C
Cuts at: 0 941 1407
Size: 941 466
BciVI GTATCCnnnnn_n'
Cuts at: 0 1069 1407
Size: 1069 338
BglI GCCn_nnn'nGGC
Cuts at: 0 978 1407
Size: 978 429
BmrI ACTGGGnnnn_n'
Cuts at: 0 169 236 1053 1407
Size: 169 67 817 354
Fragments arranged by size:
817 354 169 67
BmtI G_CTAG'C
Cuts at: 0 745 1407
Size: 745 662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 551 1212 1354 1407
Size: 441 110 661 142 53
Fragments arranged by size:
661 441 142 110 53
Bpu10I CC'TnA_GC
Cuts at: 0 746 1407
Size: 746 661
BsaI GGTCTCn'nnnn_
Cuts at: 0 459 1407
Size: 459 948
BsaWI w'CCGG_w
Cuts at: 0 1019 1407
Size: 1019 388
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 153 183 1407
Size: 153 30 1224
Fragments arranged by size:
1224 153 30
BseYI C'CCAG_C
Cuts at: 0 60 323 1407
Size: 60 263 1084
Fragments arranged by size:
1084 263 60
BsiHKAI G_wGCw'C
Cuts at: 0 941 1407
Size: 941 466
Bsp1286I G_dGCh'C
Cuts at: 0 941 1407
Size: 941 466
BspHI T'CATG_A
Cuts at: 0 476 651 1289 1407
Size: 476 175 638 118
Fragments arranged by size:
638 476 175 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 670 1407
Size: 670 737
BstYI r'GATC_y
Cuts at: 0 1221 1407
Size: 1221 186
BtsI GCAGTG_nn'
Cuts at: 0 16 1407
Size: 16 1391
ClaI AT'CG_AT
Cuts at: 0 1130 1407
Size: 1130 277
DraI TTT'AAA
Cuts at: 0 891 1407
Size: 891 516
EaeI y'GGCC_r
Cuts at: 0 660 961 1266 1407
Size: 660 301 305 141
Fragments arranged by size:
660 305 301 141
EarI CTCTTCn'nnn_
Cuts at: 0 1348 1407
Size: 1348 59
EcoICRI GAG'CTC
Cuts at: 0 939 1407
Size: 939 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 271 441 551 708 1212 1354 1407
Size: 151 120 170 110 157 504 142 53
Fragments arranged by size:
504 170 157 151 142 120 110 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 1407
Size: 307 1100
EcoRI G'AATT_C
Cuts at: 0 205 1407
Size: 205 1202
EcoRV GAT'ATC
Cuts at: 0 482 693 1407
Size: 482 211 714
Fragments arranged by size:
714 482 211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 864 1407
Size: 864 543
FspI TGC'GCA
Cuts at: 0 910 1407
Size: 910 497
FspAI rTGC'GCAy
Cuts at: 0 910 1407
Size: 910 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1407
Size: 1001 32 374
Fragments arranged by size:
1001 374 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1407
Size: 438 969
MscI TGG'CCA
Cuts at: 0 963 1268 1407
Size: 963 305 139
Fragments arranged by size:
963 305 139
MslI CAynn'nnrTG
Cuts at: 0 590 1130 1407
Size: 590 540 277
Fragments arranged by size:
590 540 277
NheI G'CTAG_C
Cuts at: 0 741 1407
Size: 741 666
NspI r_CATG'y
Cuts at: 0 909 1407
Size: 909 498
PflMI CCAn_nnn'nTGG
Cuts at: 0 571 1407
Size: 571 836
PfoI T'CCnGG_A
Cuts at: 0 1257 1407
Size: 1257 150
PstI C_TGCA'G
Cuts at: 0 1399 1407
Size: 1399 8
SacI G_AGCT'C
Cuts at: 0 941 1407
Size: 941 466
ScaI AGT'ACT
Cuts at: 0 1042 1407
Size: 1042 365
SfcI C'TryA_G
Cuts at: 0 345 1092 1395 1407
Size: 345 747 303 12
Fragments arranged by size:
747 345 303 12
StyI C'CwwG_G
Cuts at: 0 449 1082 1407
Size: 449 633 325
Fragments arranged by size:
633 449 325
TatI w'GTAC_w
Cuts at: 0 626 1040 1407
Size: 626 414 367
Fragments arranged by size:
626 414 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 153 185 1049 1081 1407
Size: 153 32 864 32 326
Fragments arranged by size:
864 326 153 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 97 1407
Size: 97 1310
XbaI T'CTAG_A
Cuts at: 0 69 1407
Size: 69 1338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 775 1407
Size: 775 632
XmnI GAAnn'nnTTC
Cuts at: 0 409 1407
Size: 409 998
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BamHI BanII BciVI BglI
BmrI BmtI BpmI Bpu10I BsaI BsaWI BsaXI BseYI
BsiHKAI Bsp1286I BspHI BstXI BstYI BtsI ClaI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FspI FspAI Hin4I MmeI MscI MslI NheI NspI
PflMI PfoI PstI SacI ScaI SfcI StyI TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BplI
BpuEI BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI CspCI DraIII DrdI EagI EciI EcoO109I FseI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XhoI XmaI ZraI