Restriction map for
pF1KB5545
(Linear) (Six-base) MAPSORT of: KIBB5545.seq Check: 2388 from: 1 to: 1872
KIBB5545.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1108 1872
Size: 1108 764
AccI GT'mk_AC
Cuts at: 0 1536 1872
Size: 1536 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 608 884 1872
Size: 248 360 276 988
Fragments arranged by size:
988 360 276 248
AlwNI CAG_nnn'CTG
Cuts at: 0 865 1872
Size: 865 1007
ApaI G_GGCC'C
Cuts at: 0 1548 1872
Size: 1548 324
ApoI r'AATT_y
Cuts at: 0 450 579 710 1148 1809 1872
Size: 450 129 131 438 661 63
Fragments arranged by size:
661 450 438 131 129 63
BanI G'GyrC_C
Cuts at: 0 160 797 1519 1872
Size: 160 637 722 353
Fragments arranged by size:
722 637 353 160
BanII G_rGCy'C
Cuts at: 0 646 1548 1872
Size: 646 902 324
Fragments arranged by size:
902 646 324
BclI T'GATC_A
Cuts at: 0 36 1872
Size: 36 1836
BfrBI ATG'CAT
Cuts at: 0 1696 1872
Size: 1696 176
BglII A'GATC_T
Cuts at: 0 721 1872
Size: 721 1151
Bme1580I G_kGCm'C
Cuts at: 0 1548 1872
Size: 1548 324
BmrI ACTGGGnnnn_n'
Cuts at: 0 1543 1872
Size: 1543 329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1872
Size: 1010 862
Bpu10I CC'TnA_GC
Cuts at: 0 1585 1872
Size: 1585 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1872
Size: 869 1003
BsaI GGTCTCn'nnnn_
Cuts at: 0 936 1043 1872
Size: 936 107 829
Fragments arranged by size:
936 829 107
BsaBI GATnn'nnATC
Cuts at: 0 543 1872
Size: 543 1329
BsaWI w'CCGG_w
Cuts at: 0 1441 1872
Size: 1441 431
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 485 515 1872
Size: 485 30 1357
Fragments arranged by size:
1357 485 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 269 639 1094 1352 1872
Size: 266 3 370 455 258 520
Fragments arranged by size:
520 455 370 266 258 3
BsiEI CG_ry'CG
Cuts at: 0 1794 1872
Size: 1794 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 189 766 1872
Size: 189 577 1106
Fragments arranged by size:
1106 577 189
Bsp1286I G_dGCh'C
Cuts at: 0 646 1548 1872
Size: 646 902 324
Fragments arranged by size:
902 646 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 883 1108 1872
Size: 883 225 764
Fragments arranged by size:
883 764 225
BsrBI CCG'CTC
Cuts at: 0 1113 1872
Size: 1113 759
BsrDI GCAATG_nn'
Cuts at: 0 1676 1872
Size: 1676 196
BsrGI T'GTAC_A
Cuts at: 0 1259 1872
Size: 1259 613
BstEII G'GTnAC_C
Cuts at: 0 686 1872
Size: 686 1186
BstYI r'GATC_y
Cuts at: 0 721 1872
Size: 721 1151
BtsI GCAGTG_nn'
Cuts at: 0 377 1782 1872
Size: 377 1405 90
Fragments arranged by size:
1405 377 90
ClaI AT'CG_AT
Cuts at: 0 1790 1872
Size: 1790 82
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 12 47 1872
Size: 12 35 1825
Fragments arranged by size:
1825 35 12
DraIII CAC_nnn'GTG
Cuts at: 0 804 1330 1872
Size: 804 526 542
Fragments arranged by size:
804 542 526
EarI CTCTTCn'nnn_
Cuts at: 0 179 267 972 1329 1872
Size: 179 88 705 357 543
Fragments arranged by size:
705 543 357 179 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 608 884 1010 1872
Size: 248 360 276 126 862
Fragments arranged by size:
862 360 276 248 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 614 1872
Size: 614 1258
EcoO109I rG'GnC_Cy
Cuts at: 0 603 1544 1545 1872
Size: 603 941 1 327
Fragments arranged by size:
941 603 327 1
EcoRI G'AATT_C
Cuts at: 0 1148 1809 1872
Size: 1148 661 63
Fragments arranged by size:
1148 661 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 896 1589 1872
Size: 896 693 283
Fragments arranged by size:
896 693 283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 1177 1209 1258 1290 1872
Size: 259 32 886 32 49 32 582
Fragments arranged by size:
886 582 259 49 32 32 32
HincII GTy'rAC
Cuts at: 0 429 596 1537 1872
Size: 429 167 941 335
Fragments arranged by size:
941 429 335 167
HindIII A'AGCT_T
Cuts at: 0 89 1872
Size: 89 1783
HpaI GTT'AAC
Cuts at: 0 429 596 1872
Size: 429 167 1276
Fragments arranged by size:
1276 429 167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 973 1872
Size: 358 615 899
Fragments arranged by size:
899 615 358
MspA1I CmG'CkG
Cuts at: 0 910 1872
Size: 910 962
NsiI A_TGCA'T
Cuts at: 0 1698 1872
Size: 1698 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 394 1872
Size: 394 1478
PfoI T'CCnGG_A
Cuts at: 0 987 1872
Size: 987 885
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1368 1386 1400 1418 1872
Size: 1368 18 14 18 454
Fragments arranged by size:
1368 454 18 18 14
PpuMI rG'GwC_Cy
Cuts at: 0 603 1872
Size: 603 1269
PspOMI G'GGCC_C
Cuts at: 0 1544 1872
Size: 1544 328
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 965 997 1350 1382 1872
Size: 965 32 353 32 490
Fragments arranged by size:
965 490 353 32 32
PstI C_TGCA'G
Cuts at: 0 48 1872
Size: 48 1824
PvuI CG_AT'CG
Cuts at: 0 1794 1872
Size: 1794 78
PvuII CAG'CTG
Cuts at: 0 910 1872
Size: 910 962
SalI G'TCGA_C
Cuts at: 0 1535 1872
Size: 1535 337
ScaI AGT'ACT
Cuts at: 0 177 1872
Size: 177 1695
SfcI C'TryA_G
Cuts at: 0 44 202 926 1707 1872
Size: 44 158 724 781 165
Fragments arranged by size:
781 724 165 158 44
SmlI C'TyrA_G
Cuts at: 0 884 1872
Size: 884 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 1019 1872
Size: 86 933 853
Fragments arranged by size:
933 853 86
TatI w'GTAC_w
Cuts at: 0 175 1259 1872
Size: 175 1084 613
Fragments arranged by size:
1084 613 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 1872
Size: 485 32 1355
Fragments arranged by size:
1355 485 32
XbaI T'CTAG_A
Cuts at: 0 565 787 1872
Size: 565 222 1085
Fragments arranged by size:
1085 565 222
XmnI GAAnn'nnTTC
Cuts at: 0 879 1872
Size: 879 993
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI BanI BanII
BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrGI BstEII BstYI BtsI ClaI
CspCI DraIII EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HincII HindIII HpaI MmeI MspA1I NsiI PflMI
PfoI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
SalI ScaI SfcI SmlI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BglI BlpI BmgBI BmtI BplI BsaAI BsaHI BseYI
BsgI BsiHKAI BsiWI BsmI BspEI BspHI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
DraI DrdI EaeI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PmlI PshAI
PsiI PspXI RsrII SacI SacII SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI Tth111I XcmI
XhoI XmaI ZraI