Restriction map for
pF1KB5529
(Linear) (Six-base) MAPSORT of: KIBB5529.seq Check: 8084 from: 1 to: 900
KIBB5529.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 816 900
Size: 816 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 900
Size: 352 548
AhdI GACnn_n'nnGTC
Cuts at: 0 126 900
Size: 126 774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 239 337 652 817 900
Size: 239 98 315 165 83
Fragments arranged by size:
315 239 165 98 83
AlwNI CAG_nnn'CTG
Cuts at: 0 384 387 900
Size: 384 3 513
Fragments arranged by size:
513 384 3
ApaI G_GGCC'C
Cuts at: 0 183 308 600 900
Size: 183 125 292 300
Fragments arranged by size:
300 292 183 125
ApaLI G'TGCA_C
Cuts at: 0 322 900
Size: 322 578
AvaI C'yCGr_G
Cuts at: 0 48 159 301 900
Size: 48 111 142 599
Fragments arranged by size:
599 142 111 48
AvrII C'CTAG_G
Cuts at: 0 753 900
Size: 753 147
BamHI G'GATC_C
Cuts at: 0 402 900
Size: 402 498
BanI G'GyrC_C
Cuts at: 0 75 900
Size: 75 825
BanII G_rGCy'C
Cuts at: 0 55 183 308 600 900
Size: 55 128 125 292 300
Fragments arranged by size:
300 292 128 125 55
BbeI G_GCGC'C
Cuts at: 0 79 900
Size: 79 821
BbvCI CC'TCA_GC
Cuts at: 0 98 271 733 900
Size: 98 173 462 167
Fragments arranged by size:
462 173 167 98
BglI GCCn_nnn'nGGC
Cuts at: 0 161 294 900
Size: 161 133 606
Fragments arranged by size:
606 161 133
Bme1580I G_kGCm'C
Cuts at: 0 183 308 326 600 900
Size: 183 125 18 274 300
Fragments arranged by size:
300 274 183 125 18
BmgBI CAC'GTC
Cuts at: 0 207 900
Size: 207 693
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 678 900
Size: 177 501 222
Fragments arranged by size:
501 222 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 54 900
Size: 54 846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 766 900
Size: 727 39 134
Fragments arranged by size:
727 134 39
Bpu10I CC'TnA_GC
Cuts at: 0 39 98 271 733 900
Size: 39 59 173 462 167
Fragments arranged by size:
462 173 167 59 39
BsaHI Gr'CG_yC
Cuts at: 0 76 169 900
Size: 76 93 731
Fragments arranged by size:
731 93 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 900
Size: 49 30 821
Fragments arranged by size:
821 49 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 605 900
Size: 605 295
BseYI C'CCAG_C
Cuts at: 0 135 522 900
Size: 135 387 378
Fragments arranged by size:
387 378 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 431 496 523 537 622 777 900
Size: 397 34 65 27 14 85 155 123
Fragments arranged by size:
397 155 123 85 65 34 27 14
BsiHKAI G_wGCw'C
Cuts at: 0 55 326 900
Size: 55 271 574
Fragments arranged by size:
574 271 55
Bsp1286I G_dGCh'C
Cuts at: 0 55 183 308 326 600 900
Size: 55 128 125 18 274 300
Fragments arranged by size:
300 274 128 125 55 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 816 900
Size: 816 84
BsrDI GCAATG_nn'
Cuts at: 0 462 468 900
Size: 462 6 432
Fragments arranged by size:
462 432 6
BsrFI r'CCGG_y
Cuts at: 0 266 293 900
Size: 266 27 607
Fragments arranged by size:
607 266 27
BssHII G'CGCG_C
Cuts at: 0 276 900
Size: 276 624
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 900
Size: 384 516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 517 900
Size: 366 151 383
Fragments arranged by size:
383 366 151
BstYI r'GATC_y
Cuts at: 0 402 900
Size: 402 498
BtgI C'CryG_G
Cuts at: 0 308 492 900
Size: 308 184 408
Fragments arranged by size:
408 308 184
BtsI GCAGTG_nn'
Cuts at: 0 535 580 900
Size: 535 45 320
Fragments arranged by size:
535 320 45
EaeI y'GGCC_r
Cuts at: 0 264 350 644 851 900
Size: 264 86 294 207 49
Fragments arranged by size:
294 264 207 86 49
EarI CTCTTCn'nnn_
Cuts at: 0 112 328 889 900
Size: 112 216 561 11
Fragments arranged by size:
561 216 112 11
EcoICRI GAG'CTC
Cuts at: 0 53 900
Size: 53 847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 727 766 900
Size: 352 375 39 134
Fragments arranged by size:
375 352 134 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 670 900
Size: 670 230
EcoO109I rG'GnC_Cy
Cuts at: 0 102 179 287 596 900
Size: 102 77 108 309 304
Fragments arranged by size:
309 304 108 102 77
HaeII r_GCGC'y
Cuts at: 0 79 753 900
Size: 79 674 147
Fragments arranged by size:
674 147 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 726 758 900
Size: 112 32 582 32 142
Fragments arranged by size:
582 142 112 32 32
KasI G'GCGC_C
Cuts at: 0 75 900
Size: 75 825
MscI TGG'CCA
Cuts at: 0 646 853 900
Size: 646 207 47
Fragments arranged by size:
646 207 47
MslI CAynn'nnrTG
Cuts at: 0 727 900
Size: 727 173
MspA1I CmG'CkG
Cuts at: 0 87 192 262 384 415 480 900
Size: 87 105 70 122 31 65 420
Fragments arranged by size:
420 122 105 87 70 65 31
NaeI GCC'GGC
Cuts at: 0 268 900
Size: 268 632
NarI GG'CG_CC
Cuts at: 0 76 900
Size: 76 824
NcoI C'CATG_G
Cuts at: 0 492 900
Size: 492 408
NdeI CA'TA_TG
Cuts at: 0 728 900
Size: 728 172
NgoMIV G'CCGG_C
Cuts at: 0 266 900
Size: 266 634
PfoI T'CCnGG_A
Cuts at: 0 485 900
Size: 485 415
PspOMI G'GGCC_C
Cuts at: 0 179 304 596 900
Size: 179 125 292 304
Fragments arranged by size:
304 292 179 125
PspXI vC'TCGA_Gb
Cuts at: 0 48 900
Size: 48 852
PstI C_TGCA'G
Cuts at: 0 671 806 878 900
Size: 671 135 72 22
Fragments arranged by size:
671 135 72 22
PvuII CAG'CTG
Cuts at: 0 384 415 480 900
Size: 384 31 65 420
Fragments arranged by size:
420 384 65 31
SacI G_AGCT'C
Cuts at: 0 55 900
Size: 55 845
SbfI CC_TGCA'GG
Cuts at: 0 671 900
Size: 671 229
SfcI C'TryA_G
Cuts at: 0 667 802 874 900
Size: 667 135 72 26
Fragments arranged by size:
667 135 72 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 161 900
Size: 161 739
SfoI GGC'GCC
Cuts at: 0 77 900
Size: 77 823
SgrAI Cr'CCGG_yG
Cuts at: 0 293 900
Size: 293 607
SmlI C'TyrA_G
Cuts at: 0 48 900
Size: 48 852
StyI C'CwwG_G
Cuts at: 0 492 753 900
Size: 492 261 147
Fragments arranged by size:
492 261 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 632 900
Size: 632 268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 900
Size: 49 32 819
Fragments arranged by size:
819 49 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 825 900
Size: 825 75
XhoI C'TCGA_G
Cuts at: 0 48 900
Size: 48 852
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApaI ApaLI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII
BstAPI BstXI BstYI BtgI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV PfoI PspOMI
PspXI PstI PvuII SacI SbfI SfcI SfiI SfoI
SgrAI SmlI StyI TaqII TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApoI AscI AseI AsiSI BaeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI BmtI BpuEI
BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XmaI XmnI ZraI