Restriction map for
pF1KB5527
(Linear) (Six-base) MAPSORT of: KIBB5527.seq Check: 8432 from: 1 to: 2268
KIBB5527.orf
With 182 enzymes: SgfI *
July 31, 2008 16:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1220 2268
Size: 1220 1048
AccI GT'mk_AC
Cuts at: 0 881 2268
Size: 881 1387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 106 526 537 843 998 1312 1330
Size: 88 18 420 11 306 155 314 18
Cuts at: 1330 1828 2268
Size: 498 440
Fragments arranged by size:
498 440 420 314 306 155 88 18
18 11
AfeI AGC'GCT
Cuts at: 0 118 769 2268
Size: 118 651 1499
Fragments arranged by size:
1499 651 118
AflIII A'CryG_T
Cuts at: 0 1760 2268
Size: 1760 508
AleI CACnn'nnGTG
Cuts at: 0 875 1372 2268
Size: 875 497 896
Fragments arranged by size:
896 875 497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 1232 2268
Size: 190 1042 1036
Fragments arranged by size:
1042 1036 190
AlwNI CAG_nnn'CTG
Cuts at: 0 63 84 742 1920 2268
Size: 63 21 658 1178 348
Fragments arranged by size:
1178 658 348 63 21
ApaI G_GGCC'C
Cuts at: 0 968 1043 1231 1871 2268
Size: 968 75 188 640 397
Fragments arranged by size:
968 640 397 188 75
ApoI r'AATT_y
Cuts at: 0 1731 1944 2268
Size: 1731 213 324
Fragments arranged by size:
1731 324 213
AvaI C'yCGr_G
Cuts at: 0 6 274 1324 2268
Size: 6 268 1050 944
Fragments arranged by size:
1050 944 268 6
BanI G'GyrC_C
Cuts at: 0 233 1208 1531 1634 1837 2268
Size: 233 975 323 103 203 431
Fragments arranged by size:
975 431 323 233 203 103
BanII G_rGCy'C
Cuts at: 0 515 670 779 968 1043 1231 1334 1475
Size: 515 155 109 189 75 188 103 141
Cuts at: 1475 1871 2268
Size: 396 397
Fragments arranged by size:
515 397 396 189 188 155 141 109
103 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 291 1391 2268
Size: 291 1100 877
Fragments arranged by size:
1100 877 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 554 2268
Size: 520 34 1714
Fragments arranged by size:
1714 520 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2179 2268
Size: 2179 89
BglI GCCn_nnn'nGGC
Cuts at: 0 1135 2268
Size: 1135 1133
BglII A'GATC_T
Cuts at: 0 548 2252 2268
Size: 548 1704 16
Fragments arranged by size:
1704 548 16
BlpI GC'TnA_GC
Cuts at: 0 904 2268
Size: 904 1364
Bme1580I G_kGCm'C
Cuts at: 0 236 275 968 996 1043 1231 1584 1637
Size: 236 39 693 28 47 188 353 53
Cuts at: 1637 1871 1881 2268
Size: 234 10 387
Fragments arranged by size:
693 387 353 236 234 188 53 47
39 28 10
BmgBI CAC'GTC
Cuts at: 0 1761 1998 2268
Size: 1761 237 270
Fragments arranged by size:
1761 270 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 2268
Size: 369 1899
BmtI G_CTAG'C
Cuts at: 0 961 1465 2268
Size: 961 504 803
Fragments arranged by size:
961 803 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 607 967 1039 1186 1204 1364 1506
Size: 271 336 360 72 147 18 160 142
Cuts at: 1506 2245 2268
Size: 739 23
Fragments arranged by size:
739 360 336 271 160 147 142 72
23 18
Bpu10I CC'TnA_GC
Cuts at: 0 669 1108 1283 1456 1639 1862 2268
Size: 669 439 175 173 183 223 406
Fragments arranged by size:
669 439 406 223 183 175 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2161 2268
Size: 2161 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 1820 2268
Size: 1820 448
BsaAI yAC'GTr
Cuts at: 0 1803 2268
Size: 1803 465
BsaBI GATnn'nnATC
Cuts at: 0 50 2268
Size: 50 2218
BsaHI Gr'CG_yC
Cuts at: 0 1138 1561 2268
Size: 1138 423 707
Fragments arranged by size:
1138 707 423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 667 697 2050 2080 2268
Size: 382 30 255 30 1353 30 188
Fragments arranged by size:
1353 382 255 188 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 610 742 1434 1665 2118 2268
Size: 123 487 132 692 231 453 150
Fragments arranged by size:
692 487 453 231 150 132 123
BseYI C'CCAG_C
Cuts at: 0 191 353 366 626 778 967 1031 2268
Size: 191 162 13 260 152 189 64 1237
Fragments arranged by size:
1237 260 191 189 162 152 64 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 358 1161 1450 2268
Size: 13 345 803 289 818
Fragments arranged by size:
818 803 345 289 13
BsiEI CG_ry'CG
Cuts at: 0 645 2268
Size: 645 1623
BsiHKAI G_wGCw'C
Cuts at: 0 515 1109 1475 2268
Size: 515 594 366 793
Fragments arranged by size:
793 594 515 366
Bsp1286I G_dGCh'C
Cuts at: 0 236 275 515 670 779 968 996 1043
Size: 236 39 240 155 109 189 28 47
Cuts at: 1043 1109 1231 1334 1475 1584 1637 1871 1881
Size: 66 122 103 141 109 53 234 10
Cuts at: 1881 2268
Size: 387
Fragments arranged by size:
387 240 236 234 189 155 141 122
109 109 103 66 53 47 39 28
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 950 1220 2268
Size: 950 270 1048
Fragments arranged by size:
1048 950 270
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 742 1007 1920 2268
Size: 363 379 265 913 348
Fragments arranged by size:
913 379 363 348 265
BstEII G'GTnAC_C
Cuts at: 0 1260 2268
Size: 1260 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1744 2268
Size: 1744 524
BstYI r'GATC_y
Cuts at: 0 51 548 1088 1301 1976 2252 2268
Size: 51 497 540 213 675 276 16
Fragments arranged by size:
675 540 497 276 213 51 16
Bsu36I CC'TnA_GG
Cuts at: 0 1361 2268
Size: 1361 907
BtgI C'CryG_G
Cuts at: 0 401 662 1065 1691 1980 2044 2268
Size: 401 261 403 626 289 64 224
Fragments arranged by size:
626 403 401 289 261 224 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 323 1115 2268
Size: 315 8 792 1153
Fragments arranged by size:
1153 792 315 8
BtsI GCAGTG_nn'
Cuts at: 0 1173 1421 2268
Size: 1173 248 847
Fragments arranged by size:
1173 847 248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2226 2261 2268
Size: 2226 35 7
Fragments arranged by size:
2226 35 7
DraIII CAC_nnn'GTG
Cuts at: 0 363 1629 2268
Size: 363 1266 639
Fragments arranged by size:
1266 639 363
DrdI GACnn_nn'nnGTC
Cuts at: 0 186 2268
Size: 186 2082
EaeI y'GGCC_r
Cuts at: 0 2000 2148 2268
Size: 2000 148 120
Fragments arranged by size:
2000 148 120
EarI CTCTTCn'nnn_
Cuts at: 0 930 1099 2268
Size: 930 169 1169
Fragments arranged by size:
1169 930 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1609 2268
Size: 1609 659
EcoICRI GAG'CTC
Cuts at: 0 513 1473 2268
Size: 513 960 795
Fragments arranged by size:
960 795 513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 106 271 526 537 607 843 967
Size: 88 18 165 255 11 70 236 124
Cuts at: 967 998 1039 1186 1204 1312 1330 1364 1506
Size: 31 41 147 18 108 18 34 142
Cuts at: 1506 1828 2245 2268
Size: 322 417 23
Fragments arranged by size:
417 322 255 236 165 147 142 124
108 88 70 41 34 31 23 18
18 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 1309 1861 2268
Size: 946 363 552 407
Fragments arranged by size:
946 552 407 363
EcoO109I rG'GnC_Cy
Cuts at: 0 739 1228 1790 1867 1868 2268
Size: 739 489 562 77 1 400
Fragments arranged by size:
739 562 489 400 77 1
EcoRI G'AATT_C
Cuts at: 0 1944 2268
Size: 1944 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 498 837 1110 1833 2157 2268
Size: 498 339 273 723 324 111
Fragments arranged by size:
723 498 339 324 273 111
HaeII r_GCGC'y
Cuts at: 0 64 120 771 857 2268
Size: 64 56 651 86 1411
Fragments arranged by size:
1411 651 86 64 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 1546 1578 2113 2145 2268
Size: 667 32 847 32 535 32 123
Fragments arranged by size:
847 667 535 123 32 32 32
HincII GTy'rAC
Cuts at: 0 1932 2268
Size: 1932 336
MfeI C'AATT_G
Cuts at: 0 1949 2268
Size: 1949 319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1703 2268
Size: 1703 565
MscI TGG'CCA
Cuts at: 0 2002 2150 2268
Size: 2002 148 118
Fragments arranged by size:
2002 148 118
MslI CAynn'nnrTG
Cuts at: 0 523 875 1214 1372 2268
Size: 523 352 339 158 896
Fragments arranged by size:
896 523 352 339 158
MspA1I CmG'CkG
Cuts at: 0 195 284 346 630 664 723 801 1920
Size: 195 89 62 284 34 59 78 1119
Cuts at: 1920 2268
Size: 348
Fragments arranged by size:
1119 348 284 195 89 78 62 59
34
NcoI C'CATG_G
Cuts at: 0 401 1980 2044 2268
Size: 401 1579 64 224
Fragments arranged by size:
1579 401 224 64
NheI G'CTAG_C
Cuts at: 0 957 1461 2268
Size: 957 504 807
Fragments arranged by size:
957 807 504
NspI r_CATG'y
Cuts at: 0 1219 2268
Size: 1219 1049
PflMI CCAn_nnn'nTGG
Cuts at: 0 104 633 891 915 1255 1629 1722 2268
Size: 104 529 258 24 340 374 93 546
Fragments arranged by size:
546 529 374 340 258 104 93 24
PfoI T'CCnGG_A
Cuts at: 0 1118 1778 2261 2268
Size: 1118 660 483 7
Fragments arranged by size:
1118 660 483 7
PshAI GACnn'nnGTC
Cuts at: 0 410 2017 2268
Size: 410 1607 251
Fragments arranged by size:
1607 410 251
PspOMI G'GGCC_C
Cuts at: 0 964 1039 1227 1867 2268
Size: 964 75 188 640 401
Fragments arranged by size:
964 640 401 188 75
PstI C_TGCA'G
Cuts at: 0 497 713 2268
Size: 497 216 1555
Fragments arranged by size:
1555 497 216
PvuII CAG'CTG
Cuts at: 0 195 346 1920 2268
Size: 195 151 1574 348
Fragments arranged by size:
1574 348 195 151
SacI G_AGCT'C
Cuts at: 0 515 1475 2268
Size: 515 960 793
Fragments arranged by size:
960 793 515
SacII CC_GC'GG
Cuts at: 0 665 2268
Size: 665 1603
SexAI A'CCwGG_T
Cuts at: 0 914 2268
Size: 914 1354
SfcI C'TryA_G
Cuts at: 0 37 55 421 493 709 1683 2268
Size: 37 18 366 72 216 974 585
Fragments arranged by size:
974 585 366 216 72 37 18
SmlI C'TyrA_G
Cuts at: 0 2176 2268
Size: 2176 92
SphI G_CATG'C
Cuts at: 0 1219 2268
Size: 1219 1049
StuI AGG'CCT
Cuts at: 0 607 1546 2268
Size: 607 939 722
Fragments arranged by size:
939 722 607
StyI C'CwwG_G
Cuts at: 0 401 791 812 1442 1980 2044 2268
Size: 401 390 21 630 538 64 224
Fragments arranged by size:
630 538 401 390 224 64 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 351 2268
Size: 351 1917
TatI w'GTAC_w
Cuts at: 0 830 2268
Size: 830 1438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 2268
Size: 382 32 1854
Fragments arranged by size:
1854 382 32
Tth111I GACn'n_nGTC
Cuts at: 0 681 1483 2268
Size: 681 802 785
Fragments arranged by size:
802 785 681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1968 2268
Size: 1968 300
XmnI GAAnn'nnTTC
Cuts at: 0 550 2140 2268
Size: 550 1590 128
Fragments arranged by size:
1590 550 128
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NheI NspI PflMI PfoI PshAI
PspOMI PstI PvuII SacI SacII SexAI SfcI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BclI BfrBI BplI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstZ17I ClaI DraI EagI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI ZraI