Restriction map for
pF1KB5520
(Linear) (Six-base) MAPSORT of: KIBB5520.seq Check: 6394 from: 1 to: 1002
KIBB5520.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 1002
Size: 886 116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 282 1002
Size: 103 179 720
Fragments arranged by size:
720 179 103
AleI CACnn'nnGTG
Cuts at: 0 84 1002
Size: 84 918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 1002
Size: 53 949
ApaI G_GGCC'C
Cuts at: 0 872 1002
Size: 872 130
ApoI r'AATT_y
Cuts at: 0 133 262 304 1002
Size: 133 129 42 698
Fragments arranged by size:
698 133 129 42
AvaI C'yCGr_G
Cuts at: 0 46 386 857 865 1002
Size: 46 340 471 8 137
Fragments arranged by size:
471 340 137 46 8
BanI G'GyrC_C
Cuts at: 0 861 949 1002
Size: 861 88 53
Fragments arranged by size:
861 88 53
BanII G_rGCy'C
Cuts at: 0 872 1002
Size: 872 130
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 353 1002
Size: 288 65 649
Fragments arranged by size:
649 288 65
BbvCI CC'TCA_GC
Cuts at: 0 716 1002
Size: 716 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 722 1002
Size: 688 34 280
Fragments arranged by size:
688 280 34
BglI GCCn_nnn'nGGC
Cuts at: 0 789 1002
Size: 789 213
Bme1580I G_kGCm'C
Cuts at: 0 864 872 1002
Size: 864 8 130
Fragments arranged by size:
864 130 8
BmgBI CAC'GTC
Cuts at: 0 533 1002
Size: 533 469
Bpu10I CC'TnA_GC
Cuts at: 0 716 1002
Size: 716 286
BsaBI GATnn'nnATC
Cuts at: 0 101 1002
Size: 101 901
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 242 245 733 847 850 1002
Size: 242 3 488 114 3 152
Fragments arranged by size:
488 242 152 114 3 3
BseYI C'CCAG_C
Cuts at: 0 755 1002
Size: 755 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1002
Size: 49 953
Bsp1286I G_dGCh'C
Cuts at: 0 864 872 1002
Size: 864 8 130
Fragments arranged by size:
864 130 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 1002
Size: 886 116
BsrFI r'CCGG_y
Cuts at: 0 763 1002
Size: 763 239
BtgI C'CryG_G
Cuts at: 0 917 1002
Size: 917 85
ClaI AT'CG_AT
Cuts at: 0 568 1002
Size: 568 434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 519 554 1002
Size: 519 35 448
Fragments arranged by size:
519 448 35
EaeI y'GGCC_r
Cuts at: 0 663 695 1002
Size: 663 32 307
Fragments arranged by size:
663 307 32
EarI CTCTTCn'nnn_
Cuts at: 0 531 1002
Size: 531 471
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 1002
Size: 760 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 282 1002
Size: 103 179 720
Fragments arranged by size:
720 179 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 715 1002
Size: 715 287
EcoO109I rG'GnC_Cy
Cuts at: 0 321 382 510 869 876 1002
Size: 321 61 128 359 7 126
Fragments arranged by size:
359 321 128 126 61 7
EcoRV GAT'ATC
Cuts at: 0 572 1002
Size: 572 430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1002
Size: 149 853
MscI TGG'CCA
Cuts at: 0 665 1002
Size: 665 337
MslI CAynn'nnrTG
Cuts at: 0 84 1002
Size: 84 918
NaeI GCC'GGC
Cuts at: 0 765 1002
Size: 765 237
NdeI CA'TA_TG
Cuts at: 0 156 1002
Size: 156 846
NgoMIV G'CCGG_C
Cuts at: 0 763 1002
Size: 763 239
NspI r_CATG'y
Cuts at: 0 40 729 1002
Size: 40 689 273
Fragments arranged by size:
689 273 40
PasI CC'CwG_GG
Cuts at: 0 872 1002
Size: 872 130
PpuMI rG'GwC_Cy
Cuts at: 0 321 382 876 1002
Size: 321 61 494 126
Fragments arranged by size:
494 321 126 61
PspOMI G'GGCC_C
Cuts at: 0 868 1002
Size: 868 134
SanDI GG'GwC_CC
Cuts at: 0 321 382 876 1002
Size: 321 61 494 126
Fragments arranged by size:
494 321 126 61
SmaI CCC'GGG
Cuts at: 0 867 1002
Size: 867 135
SphI G_CATG'C
Cuts at: 0 729 1002
Size: 729 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 866 937 1002
Size: 866 71 65
Fragments arranged by size:
866 71 65
Tth111I GACn'n_nGTC
Cuts at: 0 411 1002
Size: 411 591
XmaI C'CCGG_G
Cuts at: 0 865 1002
Size: 865 137
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI Bpu10I
BsaBI BseRI BseYI BsgI Bsp1286I BspMI BsrFI BtgI
ClaI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV MmeI MscI MslI NaeI NdeI NgoMIV NspI
PasI PpuMI PspOMI SanDI SmaI SphI TaqII Tth111I
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI DraI
DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NarI NcoI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI TstI XbaI XcmI
XhoI XmnI ZraI