Restriction map for
pF1KB5484
(Linear) (Six-base) MAPSORT of: KIBB5484.seq Check: 5614 from: 1 to: 1950
KIBB5484.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1080 1950
Size: 1080 870
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 809 1950
Size: 479 330 1141
Fragments arranged by size:
1141 479 330
AfeI AGC'GCT
Cuts at: 0 238 352 1950
Size: 238 114 1598
Fragments arranged by size:
1598 238 114
AflIII A'CryG_T
Cuts at: 0 315 1950
Size: 315 1635
AhdI GACnn_n'nnGTC
Cuts at: 0 1011 1950
Size: 1011 939
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1080 1113 1389 1428 1950
Size: 1047 33 33 276 39 522
Fragments arranged by size:
1047 522 276 39 33 33
ApaI G_GGCC'C
Cuts at: 0 8 1950
Size: 8 1942
ApoI r'AATT_y
Cuts at: 0 430 1950
Size: 430 1520
AseI AT'TA_AT
Cuts at: 0 974 1950
Size: 974 976
AvaI C'yCGr_G
Cuts at: 0 42 150 570 696 861 1315 1950
Size: 42 108 420 126 165 454 635
Fragments arranged by size:
635 454 420 165 126 108 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 277 310 1950
Size: 277 33 1640
Fragments arranged by size:
1640 277 33
BamHI G'GATC_C
Cuts at: 0 1311 1950
Size: 1311 639
BanI G'GyrC_C
Cuts at: 0 182 453 1710 1950
Size: 182 271 1257 240
Fragments arranged by size:
1257 271 240 182
BanII G_rGCy'C
Cuts at: 0 8 133 1950
Size: 8 125 1817
Fragments arranged by size:
1817 125 8
BbeI G_GCGC'C
Cuts at: 0 186 1950
Size: 186 1764
BbvCI CC'TCA_GC
Cuts at: 0 719 1950
Size: 719 1231
BclI T'GATC_A
Cuts at: 0 1757 1950
Size: 1757 193
BglI GCCn_nnn'nGGC
Cuts at: 0 44 166 1198 1950
Size: 44 122 1032 752
Fragments arranged by size:
1032 752 122 44
Bme1580I G_kGCm'C
Cuts at: 0 8 410 1950
Size: 8 402 1540
Fragments arranged by size:
1540 402 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1469 1950
Size: 1469 481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1798 1950
Size: 1798 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 620 970 1102 1520 1894 1950
Size: 295 325 350 132 418 374 56
Fragments arranged by size:
418 374 350 325 295 132 56
Bpu10I CC'TnA_GC
Cuts at: 0 719 1421 1950
Size: 719 702 529
Fragments arranged by size:
719 702 529
BsaAI yAC'GTr
Cuts at: 0 316 1950
Size: 316 1634
BsaHI Gr'CG_yC
Cuts at: 0 183 1950
Size: 183 1767
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 236 266 340 370 607 637 1078 1108
Size: 236 30 74 30 237 30 441 30
Cuts at: 1108 1950
Size: 842
Fragments arranged by size:
842 441 237 236 74 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 748 967 1099 1384 1441 1891 1950
Size: 748 219 132 285 57 450 59
Fragments arranged by size:
748 450 285 219 132 59 57
BseYI C'CCAG_C
Cuts at: 0 218 1717 1950
Size: 218 1499 233
Fragments arranged by size:
1499 233 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1950
Size: 547 1403
BsiEI CG_ry'CG
Cuts at: 0 143 216 1950
Size: 143 73 1734
Fragments arranged by size:
1734 143 73
BsiHKAI G_wGCw'C
Cuts at: 0 920 1058 1950
Size: 920 138 892
Fragments arranged by size:
920 892 138
BsmI GAATG_Cn'
Cuts at: 0 1656 1950
Size: 1656 294
Bsp1286I G_dGCh'C
Cuts at: 0 8 133 410 920 1058 1950
Size: 8 125 277 510 138 892
Fragments arranged by size:
892 510 277 138 125 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 1031 1080 1256 1950
Size: 212 819 49 176 694
Fragments arranged by size:
819 694 212 176 49
BsrBI CCG'CTC
Cuts at: 0 374 1768 1950
Size: 374 1394 182
Fragments arranged by size:
1394 374 182
BsrFI r'CCGG_y
Cuts at: 0 121 211 467 1950
Size: 121 90 256 1483
Fragments arranged by size:
1483 256 121 90
BssHII G'CGCG_C
Cuts at: 0 93 95 1950
Size: 93 2 1855
Fragments arranged by size:
1855 93 2
BssSI C'ACGA_G
Cuts at: 0 919 1950
Size: 919 1031
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 1281 1950
Size: 270 1011 669
Fragments arranged by size:
1011 669 270
BstEII G'GTnAC_C
Cuts at: 0 670 1950
Size: 670 1280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 345 1482 1950
Size: 169 176 1137 468
Fragments arranged by size:
1137 468 176 169
BstYI r'GATC_y
Cuts at: 0 1203 1311 1950
Size: 1203 108 639
Fragments arranged by size:
1203 639 108
Bsu36I CC'TnA_GG
Cuts at: 0 931 1211 1950
Size: 931 280 739
Fragments arranged by size:
931 739 280
BtgI C'CryG_G
Cuts at: 0 344 606 998 1307 1839 1950
Size: 344 262 392 309 532 111
Fragments arranged by size:
532 392 344 309 262 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1125 1950
Size: 1125 825
BtsI GCAGTG_nn'
Cuts at: 0 842 1950
Size: 842 1108
DrdI GACnn_nn'nnGTC
Cuts at: 0 1728 1950
Size: 1728 222
EaeI y'GGCC_r
Cuts at: 0 119 140 213 1301 1950
Size: 119 21 73 1088 649
Fragments arranged by size:
1088 649 119 73 21
EagI C'GGCC_G
Cuts at: 0 140 213 1950
Size: 140 73 1737
Fragments arranged by size:
1737 140 73
EarI CTCTTCn'nnn_
Cuts at: 0 725 785 791 1950
Size: 725 60 6 1159
Fragments arranged by size:
1159 725 60 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1196 1231 1787 1950
Size: 1196 35 556 163
Fragments arranged by size:
1196 556 163 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 479 620 809 970 1102 1520 1894
Size: 295 184 141 189 161 132 418 374
Cuts at: 1894 1950
Size: 56
Fragments arranged by size:
418 374 295 189 184 161 141 132
56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1420 1950
Size: 1420 530
EcoO109I rG'GnC_Cy
Cuts at: 0 4 147 201 258 444 552 641 1044
Size: 4 143 54 57 186 108 89 403
Cuts at: 1044 1471 1950
Size: 427 479
Fragments arranged by size:
479 427 403 186 143 108 89 57
54 4
FspI TGC'GCA
Cuts at: 0 208 1810 1950
Size: 208 1602 140
Fragments arranged by size:
1602 208 140
HaeII r_GCGC'y
Cuts at: 0 186 240 354 1113 1164 1245 1950
Size: 186 54 114 759 51 81 705
Fragments arranged by size:
759 705 186 114 81 54 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 724 756 997 1029 1078 1110
Size: 236 32 456 32 241 32 49 32
Cuts at: 1110 1365 1397 1582 1614 1729 1761 1950
Size: 255 32 185 32 115 32 189
Fragments arranged by size:
456 255 241 236 189 185 115 49
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 182 1950
Size: 182 1768
MslI CAynn'nnrTG
Cuts at: 0 343 1558 1910 1950
Size: 343 1215 352 40
Fragments arranged by size:
1215 352 343 40
MspA1I CmG'CkG
Cuts at: 0 19 57 63 218 380 416 1626 1717
Size: 19 38 6 155 162 36 1210 91
Cuts at: 1717 1902 1950
Size: 185 48
Fragments arranged by size:
1210 185 162 155 91 48 38 36
19 6
NarI GG'CG_CC
Cuts at: 0 183 1950
Size: 183 1767
NcoI C'CATG_G
Cuts at: 0 344 998 1839 1950
Size: 344 654 841 111
Fragments arranged by size:
841 654 344 111
NspI r_CATG'y
Cuts at: 0 579 900 1300 1950
Size: 579 321 400 650
Fragments arranged by size:
650 579 400 321
PasI CC'CwG_GG
Cuts at: 0 549 1498 1950
Size: 549 949 452
Fragments arranged by size:
949 549 452
PflMI CCAn_nnn'nTGG
Cuts at: 0 1299 1845 1950
Size: 1299 546 105
Fragments arranged by size:
1299 546 105
PfoI T'CCnGG_A
Cuts at: 0 254 367 1442 1934 1950
Size: 254 113 1075 492 16
Fragments arranged by size:
1075 492 254 113 16
PmlI CAC'GTG
Cuts at: 0 316 1950
Size: 316 1634
PpuMI rG'GwC_Cy
Cuts at: 0 201 258 641 1471 1950
Size: 201 57 383 830 479
Fragments arranged by size:
830 479 383 201 57
PspOMI G'GGCC_C
Cuts at: 0 4 1950
Size: 4 1946
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1909 1941 1950
Size: 1909 32 9
Fragments arranged by size:
1909 32 9
PstI C_TGCA'G
Cuts at: 0 286 308 544 1076 1199 1532 1901 1950
Size: 286 22 236 532 123 333 369 49
Fragments arranged by size:
532 369 333 286 236 123 49 22
PvuII CAG'CTG
Cuts at: 0 380 1717 1950
Size: 380 1337 233
Fragments arranged by size:
1337 380 233
SanDI GG'GwC_CC
Cuts at: 0 258 641 1471 1950
Size: 258 383 830 479
Fragments arranged by size:
830 479 383 258
SbfI CC_TGCA'GG
Cuts at: 0 1199 1950
Size: 1199 751
ScaI AGT'ACT
Cuts at: 0 280 1950
Size: 280 1670
SexAI A'CCwGG_T
Cuts at: 0 398 1950
Size: 398 1552
SfcI C'TryA_G
Cuts at: 0 282 304 540 1072 1195 1528 1897 1950
Size: 282 22 236 532 123 333 369 53
Fragments arranged by size:
532 369 333 282 236 123 53 22
SfoI GGC'GCC
Cuts at: 0 184 1950
Size: 184 1766
SmaI CCC'GGG
Cuts at: 0 44 152 698 1950
Size: 44 108 546 1252
Fragments arranged by size:
1252 546 108 44
SphI G_CATG'C
Cuts at: 0 579 900 1950
Size: 579 321 1050
Fragments arranged by size:
1050 579 321
SrfI GCCC'GGGC
Cuts at: 0 44 1950
Size: 44 1906
StuI AGG'CCT
Cuts at: 0 538 1950
Size: 538 1412
StyI C'CwwG_G
Cuts at: 0 344 998 1172 1544 1663 1839 1950
Size: 344 654 174 372 119 176 111
Fragments arranged by size:
654 372 344 176 174 119 111
TatI w'GTAC_w
Cuts at: 0 278 1950
Size: 278 1672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 607 639 1048 1080 1950
Size: 340 32 235 32 409 32 870
Fragments arranged by size:
870 409 340 235 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 248 1950
Size: 248 1702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 876 945 1869 1950
Size: 114 762 69 924 81
Fragments arranged by size:
924 762 114 81 69
XmaI C'CCGG_G
Cuts at: 0 42 150 696 1950
Size: 42 108 546 1254
Fragments arranged by size:
1254 546 108 42
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AlwNI ApaI ApoI
AseI AvaI BaeI BamHI BanI BanII BbeI BbvCI
BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII
SanDI SbfI ScaI SexAI SfcI SfoI SmaI SphI
SrfI StuI StyI TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AlfI
AloI ApaLI AscI AsiSI AvrII BbsI BcgI BciVI
BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI
BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BstBI
BstZ17I ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SapI SfiI
SgfI SgrAI SmlI SnaBI SpeI SspI SwaI TaqII
XbaI XhoI XmnI ZraI