(Linear) (Six-base) MAPSORT of: KIBB5482.seq  Check: 5023  from: 1  to: 735



KIBB5482.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AflII C'TTAA_G

Cuts at:      0     196     735 
   Size:        196     539
 
AleI CACnn'nnGTG

Cuts at:      0     417     735 
   Size:        417     318
 
ApoI r'AATT_y

Cuts at:      0     424     735 
   Size:        424     311
 
AvaI C'yCGr_G

Cuts at:      0     140     735 
   Size:        140     595
 
BanI G'GyrC_C

Cuts at:      0     271     735 
   Size:        271     464
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     186     735 
   Size:        186     549
 
BbvCI CC'TCA_GC

Cuts at:      0     191     735 
   Size:        191     544
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     379     735 
   Size:        379     356
 
BglI GCCn_nnn'nGGC

Cuts at:      0     230     735 
   Size:        230     505
 
Bpu10I CC'TnA_GC

Cuts at:      0     191     735 
   Size:        191     544
 
BseYI C'CCAG_C

Cuts at:      0      91     735 
   Size:         91     644
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610     735 
   Size:        610     125
 
BspHI T'CATG_A

Cuts at:      0     536     735 
   Size:        536     199
 
BsrDI GCAATG_nn'

Cuts at:      0     377     735 
   Size:        377     358
 
BsrGI T'GTAC_A

Cuts at:      0     392     608     735 
   Size:        392     216     127

  Fragments arranged by size: 

                 392     216     127
 
BtsI GCAGTG_nn'

Cuts at:      0     622     735 
   Size:        622     113
 
EaeI y'GGCC_r

Cuts at:      0     602     735 
   Size:        602     133
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     157     160     735 
   Size:         31     126       3     575

  Fragments arranged by size: 

                 575     126      31       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     268     735 
   Size:        268     467
 
EcoO109I rG'GnC_Cy

Cuts at:      0     662     735 
   Size:        662      73
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     381     735 
   Size:        381     354
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     465     497     735 
   Size:        465      32     238

  Fragments arranged by size: 

                 465     238      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     735 
   Size:         89     646
 
MscI TGG'CCA

Cuts at:      0     604     735 
   Size:        604     131
 
MslI CAynn'nnrTG

Cuts at:      0     316     417     735 
   Size:        316     101     318

  Fragments arranged by size: 

                 318     316     101
 
MspA1I CmG'CkG

Cuts at:      0     110     246     735 
   Size:        110     136     489

  Fragments arranged by size: 

                 489     136     110
 
PpuMI rG'GwC_Cy

Cuts at:      0     662     735 
   Size:        662      73
 
PstI C_TGCA'G

Cuts at:      0     251     573     735 
   Size:        251     322     162

  Fragments arranged by size: 

                 322     251     162
 
PvuII CAG'CTG

Cuts at:      0     110     246     735 
   Size:        110     136     489

  Fragments arranged by size: 

                 489     136     110
 
SanDI GG'GwC_CC

Cuts at:      0     662     735 
   Size:        662      73
 
SfcI C'TryA_G

Cuts at:      0     247     569     735 
   Size:        247     322     166

  Fragments arranged by size: 

                 322     247     166
 
SmaI CCC'GGG

Cuts at:      0     142     735 
   Size:        142     593
 
SmlI C'TyrA_G

Cuts at:      0     196     735 
   Size:        196     539
 
StyI C'CwwG_G

Cuts at:      0     342     735 
   Size:        342     393
 
TatI w'GTAC_w

Cuts at:      0     362     392     608     735 
   Size:        362      30     216     127

  Fragments arranged by size: 

                 362     216     127      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     363     735 
   Size:        363     372
 
XmaI C'CCGG_G

Cuts at:      0     140     735 
   Size:        140     595
 

 Enzymes that do cut:

    AflII     AleI     ApoI     AvaI     BanI     BbsI    BbvCI    BciVI
     BglI   Bpu10I    BseYI     BsgI    BspHI    BsrDI    BsrGI     BtsI
     EaeI     EarI     EciI EcoO109I     FalI    Hin4I     MmeI     MscI
     MslI   MspA1I    PpuMI     PstI    PvuII    SanDI     SfcI     SmaI
     SmlI     StyI     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI