Restriction map for
pF1KB5479
(Linear) (Six-base) MAPSORT of: KIBB5479.seq Check: 8962 from: 1 to: 1458
KIBB5479.orf
With 182 enzymes: SgfI *
March 31, 2008 15:57 ..
Acc65I G'GTAC_C
Cuts at: 0 384 943 1458
Size: 384 559 515
Fragments arranged by size:
559 515 384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 541 833 1220 1458
Size: 410 131 292 387 238
Fragments arranged by size:
410 387 292 238 131
AleI CACnn'nnGTG
Cuts at: 0 593 1458
Size: 593 865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 940 1458
Size: 940 518
AlwNI CAG_nnn'CTG
Cuts at: 0 447 582 1458
Size: 447 135 876
Fragments arranged by size:
876 447 135
ApaLI G'TGCA_C
Cuts at: 0 586 1458
Size: 586 872
AvaI C'yCGr_G
Cuts at: 0 1259 1458
Size: 1259 199
BanI G'GyrC_C
Cuts at: 0 384 402 484 943 1172 1276 1458
Size: 384 18 82 459 229 104 182
Fragments arranged by size:
459 384 229 182 104 82 18
BanII G_rGCy'C
Cuts at: 0 616 1458
Size: 616 842
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 1398 1458
Size: 821 577 60
Fragments arranged by size:
821 577 60
BciVI GTATCCnnnnn_n'
Cuts at: 0 448 1458
Size: 448 1010
BglI GCCn_nnn'nGGC
Cuts at: 0 1062 1458
Size: 1062 396
BglII A'GATC_T
Cuts at: 0 746 1458
Size: 746 712
Bme1580I G_kGCm'C
Cuts at: 0 590 726 744 1072 1281 1293 1458
Size: 590 136 18 328 209 12 165
Fragments arranged by size:
590 328 209 165 136 18 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 156 1458
Size: 156 1302
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 311 491 1458
Size: 311 180 967
Fragments arranged by size:
967 311 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 123 246 289 461 1458
Size: 94 29 123 43 172 997
Fragments arranged by size:
997 172 123 94 43 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1288 1458
Size: 658 630 170
Fragments arranged by size:
658 630 170
BsaWI w'CCGG_w
Cuts at: 0 284 965 1458
Size: 284 681 493
Fragments arranged by size:
681 493 284
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1075 1458
Size: 1045 30 383
Fragments arranged by size:
1045 383 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1063 1066 1458
Size: 1063 3 392
Fragments arranged by size:
1063 392 3
BseYI C'CCAG_C
Cuts at: 0 3 148 374 1458
Size: 3 145 226 1084
Fragments arranged by size:
1084 226 145 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1228 1397 1458
Size: 1001 227 169 61
Fragments arranged by size:
1001 227 169 61
BsiHKAI G_wGCw'C
Cuts at: 0 590 616 1458
Size: 590 26 842
Fragments arranged by size:
842 590 26
BsmI GAATG_Cn'
Cuts at: 0 975 1458
Size: 975 483
Bsp1286I G_dGCh'C
Cuts at: 0 590 616 726 744 1072 1281 1293 1458
Size: 590 26 110 18 328 209 12 165
Fragments arranged by size:
590 328 209 165 110 26 18 12
BspHI T'CATG_A
Cuts at: 0 1010 1458
Size: 1010 448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 827 1458
Size: 115 712 631
Fragments arranged by size:
712 631 115
BsrBI CCG'CTC
Cuts at: 0 365 1458
Size: 365 1093
BsrDI GCAATG_nn'
Cuts at: 0 713 1458
Size: 713 745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 912 918 1320 1458
Size: 33 879 6 402 138
Fragments arranged by size:
879 402 138 33 6
BstYI r'GATC_y
Cuts at: 0 110 224 253 746 1458
Size: 110 114 29 493 712
Fragments arranged by size:
712 493 114 110 29
BtgI C'CryG_G
Cuts at: 0 823 911 1319 1458
Size: 823 88 408 139
Fragments arranged by size:
823 408 139 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 59 1113 1458
Size: 59 1054 345
Fragments arranged by size:
1054 345 59
BtsI GCAGTG_nn'
Cuts at: 0 570 892 1072 1458
Size: 570 322 180 386
Fragments arranged by size:
570 386 322 180
DraIII CAC_nnn'GTG
Cuts at: 0 164 1458
Size: 164 1294
EaeI y'GGCC_r
Cuts at: 0 23 1101 1458
Size: 23 1078 357
Fragments arranged by size:
1078 357 23
EarI CTCTTCn'nnn_
Cuts at: 0 386 406 1458
Size: 386 20 1052
Fragments arranged by size:
1052 386 20
EcoICRI GAG'CTC
Cuts at: 0 614 1458
Size: 614 844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 123 246 289 410 461 541 833
Size: 94 29 123 43 121 51 80 292
Cuts at: 833 1220 1458
Size: 387 238
Fragments arranged by size:
387 292 238 123 121 94 80 51
43 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1458
Size: 523 935
EcoO109I rG'GnC_Cy
Cuts at: 0 201 396 978 1336 1458
Size: 201 195 582 358 122
Fragments arranged by size:
582 358 201 195 122
FspI TGC'GCA
Cuts at: 0 1161 1458
Size: 1161 297
HaeII r_GCGC'y
Cuts at: 0 174 1458
Size: 174 1284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 752 784 790 822 1458
Size: 752 32 6 32 636
Fragments arranged by size:
752 636 32 32 6
HincII GTy'rAC
Cuts at: 0 39 1458
Size: 39 1419
KpnI G_GTAC'C
Cuts at: 0 388 947 1458
Size: 388 559 511
Fragments arranged by size:
559 511 388
MscI TGG'CCA
Cuts at: 0 25 1458
Size: 25 1433
MslI CAynn'nnrTG
Cuts at: 0 511 593 595 916 922 1458
Size: 511 82 2 321 6 536
Fragments arranged by size:
536 511 321 82 6 2
MspA1I CmG'CkG
Cuts at: 0 152 447 1458
Size: 152 295 1011
Fragments arranged by size:
1011 295 152
NcoI C'CATG_G
Cuts at: 0 911 1319 1458
Size: 911 408 139
Fragments arranged by size:
911 408 139
NdeI CA'TA_TG
Cuts at: 0 1451 1458
Size: 1451 7
NspI r_CATG'y
Cuts at: 0 21 145 510 1458
Size: 21 124 365 948
Fragments arranged by size:
948 365 124 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 333 447 582 1458
Size: 72 261 114 135 876
Fragments arranged by size:
876 261 135 114 72
PpuMI rG'GwC_Cy
Cuts at: 0 201 396 978 1336 1458
Size: 201 195 582 358 122
Fragments arranged by size:
582 358 201 195 122
PshAI GACnn'nnGTC
Cuts at: 0 68 560 1458
Size: 68 492 898
Fragments arranged by size:
898 492 68
PspXI vC'TCGA_Gb
Cuts at: 0 1259 1458
Size: 1259 199
PstI C_TGCA'G
Cuts at: 0 126 1257 1458
Size: 126 1131 201
Fragments arranged by size:
1131 201 126
PvuII CAG'CTG
Cuts at: 0 447 1458
Size: 447 1011
SacI G_AGCT'C
Cuts at: 0 616 1458
Size: 616 842
SanDI GG'GwC_CC
Cuts at: 0 978 1458
Size: 978 480
SapI GCTCTTCn'nnn_
Cuts at: 0 406 1458
Size: 406 1052
SfcI C'TryA_G
Cuts at: 0 122 1253 1458
Size: 122 1131 205
Fragments arranged by size:
1131 205 122
SmlI C'TyrA_G
Cuts at: 0 673 1259 1303 1458
Size: 673 586 44 155
Fragments arranged by size:
673 586 155 44
StuI AGG'CCT
Cuts at: 0 1270 1458
Size: 1270 188
StyI C'CwwG_G
Cuts at: 0 617 707 905 911 1319 1458
Size: 617 90 198 6 408 139
Fragments arranged by size:
617 408 198 139 90 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 1458
Size: 175 1283
TatI w'GTAC_w
Cuts at: 0 1042 1458
Size: 1042 416
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1458
Size: 1045 32 381
Fragments arranged by size:
1045 381 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 478 485 1458
Size: 478 7 973
Fragments arranged by size:
973 478 7
XhoI C'TCGA_G
Cuts at: 0 1259 1458
Size: 1259 199
Enzymes that do cut:
Acc65I AcuI AleI AlfI AlwNI ApaLI AvaI BanI
BanII BbsI BciVI BglI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KpnI MscI
MslI MspA1I NcoI NdeI NspI PflMI PpuMI PshAI
PspXI PstI PvuII SacI SanDI SapI SfcI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BlpI
BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmaI XmnI ZraI