(Linear) (Six-base) MAPSORT of: KIBB5476.seq  Check: 598  from: 1  to: 441



KIBB5476.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

ApoI r'AATT_y

Cuts at:      0     215     441 
   Size:        215     226
 
AseI AT'TA_AT

Cuts at:      0      17     441 
   Size:         17     424
 
AvaI C'yCGr_G

Cuts at:      0     413     441 
   Size:        413      28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     237     271     441 
   Size:        237      34     170

  Fragments arranged by size: 

                 237     170      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     441 
   Size:         35     406
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      58     441 
   Size:         58     383
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     441 
   Size:        111     330
 
BstYI r'GATC_y

Cuts at:      0     374     441 
   Size:        374      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     194     441 
   Size:        194     247
 
BtsI GCAGTG_nn'

Cuts at:      0     322     441 
   Size:        322     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     441 
   Size:         35     406
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     441 
   Size:        115     326
 
HaeII r_GCGC'y

Cuts at:      0       7     441 
   Size:          7     434
 
MslI CAynn'nnrTG

Cuts at:      0     109     327     441 
   Size:        109     218     114

  Fragments arranged by size: 

                 218     114     109
 
PpuMI rG'GwC_Cy

Cuts at:      0     115     441 
   Size:        115     326
 
PstI C_TGCA'G

Cuts at:      0      69     441 
   Size:         69     372
 
SfcI C'TryA_G

Cuts at:      0      65     170     441 
   Size:         65     105     271

  Fragments arranged by size: 

                 271     105      65
 
SspI AAT'ATT

Cuts at:      0     231     441 
   Size:        231     210
 
TatI w'GTAC_w

Cuts at:      0     398     441 
   Size:        398      43
 

 Enzymes that do cut:

     ApoI     AseI     AvaI     BcgI     BpmI    BspMI    BstXI    BstYI
   Bsu36I     BtsI  Eco57MI EcoO109I    HaeII     MslI    PpuMI     PstI
     SfcI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI