(Linear) (Six-base) MAPSORT of: KIBB5474.seq  Check: 2243  from: 1  to: 738



KIBB5474.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416     738 
   Size:        416     322
 
ApoI r'AATT_y

Cuts at:      0     522     738 
   Size:        522     216
 
BanI G'GyrC_C

Cuts at:      0     163     738 
   Size:        163     575
 
BbeI G_GCGC'C

Cuts at:      0     167     738 
   Size:        167     571
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     609     738 
   Size:        609     129
 
BclI T'GATC_A

Cuts at:      0     653     738 
   Size:        653      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85     738 
   Size:         85     653
 
BlpI GC'TnA_GC

Cuts at:      0      44      53     738 
   Size:         44       9     685

  Fragments arranged by size: 

                 685      44       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     319     428     738 
   Size:        177     142     109     310

  Fragments arranged by size: 

                 310     177     142     109
 
BsaHI Gr'CG_yC

Cuts at:      0     164     738 
   Size:        164     574
 
BseYI C'CCAG_C

Cuts at:      0     440     721     738 
   Size:        440     281      17

  Fragments arranged by size: 

                 440     281      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     701     710     738 
   Size:        701       9      28

  Fragments arranged by size: 

                 701      28       9
 
BsrBI CCG'CTC

Cuts at:      0     173     738 
   Size:        173     565
 
BsrDI GCAATG_nn'

Cuts at:      0     294     738 
   Size:        294     444
 
DraIII CAC_nnn'GTG

Cuts at:      0     686     738 
   Size:        686      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     116     180     623     738 
   Size:        116      64     443     115

  Fragments arranged by size: 

                 443     116     115      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     319     416     428     738 
   Size:        177     142      97      12     310

  Fragments arranged by size: 

                 310     177     142      97      12
 
HaeII r_GCGC'y

Cuts at:      0     167     738 
   Size:        167     571
 
KasI G'GCGC_C

Cuts at:      0     163     738 
   Size:        163     575
 
MfeI C'AATT_G

Cuts at:      0     602     738 
   Size:        602     136
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     287     738 
   Size:        140     147     451

  Fragments arranged by size: 

                 451     147     140
 
NarI GG'CG_CC

Cuts at:      0     164     738 
   Size:        164     574
 
PsiI TTA'TAA

Cuts at:      0     638     738 
   Size:        638     100
 
PstI C_TGCA'G

Cuts at:      0      78     278     738 
   Size:         78     200     460

  Fragments arranged by size: 

                 460     200      78
 
SapI GCTCTTCn'nnn_

Cuts at:      0     180     738 
   Size:        180     558
 
SfcI C'TryA_G

Cuts at:      0      74     274     570     738 
   Size:         74     200     296     168

  Fragments arranged by size: 

                 296     200     168      74
 
SfoI GGC'GCC

Cuts at:      0     165     738 
   Size:        165     573
 
StuI AGG'CCT

Cuts at:      0     568     738 
   Size:        568     170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     512     738 
   Size:        512     226
 

 Enzymes that do cut:

     AcuI     ApoI     BanI     BbeI    BciVI     BclI     BglI     BlpI
     BpmI    BsaHI    BseYI    BsmBI    BsrBI    BsrDI   DraIII     EarI
  Eco57MI    HaeII     KasI     MfeI     MmeI     NarI     PsiI     PstI
     SapI     SfcI     SfoI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BfrBI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MluI     MscI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI