Restriction map for
pF1KB5462
(Linear) (Six-base) MAPSORT of: KIBB5462.seq Check: 8395 from: 1 to: 1128
KIBB5462.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 0 569 1128
Size: 569 559
AccI GT'mk_AC
Cuts at: 0 278 1128
Size: 278 850
AflIII A'CryG_T
Cuts at: 0 722 1128
Size: 722 406
ApaI G_GGCC'C
Cuts at: 0 774 1128
Size: 774 354
AvaI C'yCGr_G
Cuts at: 0 348 775 1128
Size: 348 427 353
Fragments arranged by size:
427 353 348
BanII G_rGCy'C
Cuts at: 0 153 774 824 1128
Size: 153 621 50 304
Fragments arranged by size:
621 304 153 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 148 1128
Size: 114 34 980
Fragments arranged by size:
980 114 34
BglII A'GATC_T
Cuts at: 0 986 1128
Size: 986 142
Bme1580I G_kGCm'C
Cuts at: 0 506 774 1128
Size: 506 268 354
Fragments arranged by size:
506 354 268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1128
Size: 1116 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 175 721 1128
Size: 167 8 546 407
Fragments arranged by size:
546 407 167 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 74 382 926 1128
Size: 74 308 544 202
Fragments arranged by size:
544 308 202 74
BsaHI Gr'CG_yC
Cuts at: 0 566 1128
Size: 566 562
BsaWI w'CCGG_w
Cuts at: 0 641 1128
Size: 641 487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 1128
Size: 265 30 833
Fragments arranged by size:
833 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 316 325 1128
Size: 202 114 9 803
Fragments arranged by size:
803 202 114 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 743 1128
Size: 736 7 385
Fragments arranged by size:
736 385 7
BsiEI CG_ry'CG
Cuts at: 0 124 561 1128
Size: 124 437 567
Fragments arranged by size:
567 437 124
BsiHKAI G_wGCw'C
Cuts at: 0 194 230 326 434 899 1128
Size: 194 36 96 108 465 229
Fragments arranged by size:
465 229 194 108 96 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 697 1128
Size: 573 124 431
Fragments arranged by size:
573 431 124
Bsp1286I G_dGCh'C
Cuts at: 0 153 194 230 326 434 506 774 824
Size: 153 41 36 96 108 72 268 50
Cuts at: 824 899 1128
Size: 75 229
Fragments arranged by size:
268 229 153 108 96 75 72 50
41 36
BspEI T'CCGG_A
Cuts at: 0 641 1128
Size: 641 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 875 1128
Size: 875 253
BsrBI CCG'CTC
Cuts at: 0 342 1128
Size: 342 786
BsrDI GCAATG_nn'
Cuts at: 0 551 1128
Size: 551 577
BsrFI r'CCGG_y
Cuts at: 0 402 556 1128
Size: 402 154 572
Fragments arranged by size:
572 402 154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 429 1128
Size: 321 108 699
Fragments arranged by size:
699 321 108
BstYI r'GATC_y
Cuts at: 0 986 1128
Size: 986 142
BtgI C'CryG_G
Cuts at: 0 221 1128
Size: 221 907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3 1099 1128
Size: 3 1096 29
Fragments arranged by size:
1096 29 3
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1005 1040 1128
Size: 1005 35 88
Fragments arranged by size:
1005 88 35
DraIII CAC_nnn'GTG
Cuts at: 0 234 1128
Size: 234 894
EaeI y'GGCC_r
Cuts at: 0 121 558 1128
Size: 121 437 570
Fragments arranged by size:
570 437 121
EagI C'GGCC_G
Cuts at: 0 121 558 1128
Size: 121 437 570
Fragments arranged by size:
570 437 121
EarI CTCTTCn'nnn_
Cuts at: 0 172 1085 1128
Size: 172 913 43
Fragments arranged by size:
913 172 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 175 721 1128
Size: 167 8 546 407
Fragments arranged by size:
546 407 167 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 1128
Size: 331 797
EcoO109I rG'GnC_Cy
Cuts at: 0 162 336 754 771 1128
Size: 162 174 418 17 357
Fragments arranged by size:
418 357 174 162 17
EcoRV GAT'ATC
Cuts at: 0 975 1128
Size: 975 153
FspI TGC'GCA
Cuts at: 0 595 1128
Size: 595 533
HincII GTy'rAC
Cuts at: 0 609 1128
Size: 609 519
HindIII A'AGCT_T
Cuts at: 0 958 1128
Size: 958 170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 881 1128
Size: 606 275 247
Fragments arranged by size:
606 275 247
MslI CAynn'nnrTG
Cuts at: 0 136 418 544 1128
Size: 136 282 126 584
Fragments arranged by size:
584 282 136 126
MspA1I CmG'CkG
Cuts at: 0 294 1128
Size: 294 834
NaeI GCC'GGC
Cuts at: 0 404 558 1128
Size: 404 154 570
Fragments arranged by size:
570 404 154
NcoI C'CATG_G
Cuts at: 0 221 1128
Size: 221 907
NgoMIV G'CCGG_C
Cuts at: 0 402 556 1128
Size: 402 154 572
Fragments arranged by size:
572 402 154
NspI r_CATG'y
Cuts at: 0 135 1128
Size: 135 993
PflMI CCAn_nnn'nTGG
Cuts at: 0 33 831 1128
Size: 33 798 297
Fragments arranged by size:
798 297 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 769 801 1128
Size: 769 32 327
Fragments arranged by size:
769 327 32
PpuMI rG'GwC_Cy
Cuts at: 0 162 1128
Size: 162 966
PspOMI G'GGCC_C
Cuts at: 0 770 1128
Size: 770 358
PstI C_TGCA'G
Cuts at: 0 299 1128
Size: 299 829
PvuII CAG'CTG
Cuts at: 0 294 1128
Size: 294 834
SfcI C'TryA_G
Cuts at: 0 295 1128
Size: 295 833
StyI C'CwwG_G
Cuts at: 0 221 284 1128
Size: 221 63 844
Fragments arranged by size:
844 221 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 950 1059 1128
Size: 950 109 69
Fragments arranged by size:
950 109 69
TatI w'GTAC_w
Cuts at: 0 719 1010 1128
Size: 719 291 118
Fragments arranged by size:
719 291 118
Tth111I GACn'n_nGTC
Cuts at: 0 487 1128
Size: 487 641
XbaI T'CTAG_A
Cuts at: 0 471 1128
Size: 471 657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 543 1128
Size: 30 513 585
Fragments arranged by size:
585 513 30
ZraI GAC'GTC
Cuts at: 0 567 1128
Size: 567 561
Enzymes that do cut:
AatII AccI AflIII ApaI AvaI BanII BcgI BglII
Bme1580I BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstAPI BstYI BtgI BtgZI CspCI DraIII
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRV FspI
HincII HindIII MmeI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PpiI PpuMI PspOMI PstI PvuII SfcI
StyI TaqII TatI Tth111I XbaI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaAI BsaBI BseYI BsiWI BsmI BspHI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI DraI DrdI EciI EcoICRI EcoRI FalI
FseI FspAI HaeII Hin4I HpaI KasI KpnI MfeI
MluI MscI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XhoI XmaI XmnI