(Linear) (Six-base) MAPSORT of: KIBB5459.seq  Check: 6710  from: 1  to: 1173



KIBB5459.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     362    1173 
   Size:        362     811
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1173 
   Size:       1037     136
 
AflIII A'CryG_T

Cuts at:      0     610    1173 
   Size:        610     563
 
AvaI C'yCGr_G

Cuts at:      0     393    1173 
   Size:        393     780
 
BamHI G'GATC_C

Cuts at:      0     134     175     850    1143    1173 
   Size:        134      41     675     293      30

  Fragments arranged by size: 

                 675     293     134      41      30
 
BanI G'GyrC_C

Cuts at:      0     471    1173 
   Size:        471     702
 
BanII G_rGCy'C

Cuts at:      0    1039    1173 
   Size:       1039     134
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     224     242    1173 
   Size:        224      18     931

  Fragments arranged by size: 

                 931     224      18
 
BclI T'GATC_A

Cuts at:      0     266    1173 
   Size:        266     907
 
BglI GCCn_nnn'nGGC

Cuts at:      0     646    1173 
   Size:        646     527
 
BglII A'GATC_T

Cuts at:      0     960    1173 
   Size:        960     213
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     378    1173 
   Size:        378     795
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392    1058    1173 
   Size:        392     666     115

  Fragments arranged by size: 

                 666     392     115
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     267    1173 
   Size:        267     906
 
BsaBI GATnn'nnATC

Cuts at:      0     265     586    1173 
   Size:        265     321     587

  Fragments arranged by size: 

                 587     321     265
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     980    1037    1173 
   Size:        980      57     136

  Fragments arranged by size: 

                 980     136      57
 
BseYI C'CCAG_C

Cuts at:      0     179    1173 
   Size:        179     994
 
Bsp1286I G_dGCh'C

Cuts at:      0    1039    1173 
   Size:       1039     134
 
BspHI T'CATG_A

Cuts at:      0     269    1173 
   Size:        269     904
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     215     881    1173 
   Size:        215     666     292

  Fragments arranged by size: 

                 666     292     215
 
BssSI C'ACGA_G

Cuts at:      0     116    1173 
   Size:        116    1057
 
BstYI r'GATC_y

Cuts at:      0     134     175     438     850     960    1143    1173 
   Size:        134      41     263     412     110     183      30

  Fragments arranged by size: 

                 412     263     183     134     110      41      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     317    1173 
   Size:        317     856
 
BtsI GCAGTG_nn'

Cuts at:      0      72     190    1173 
   Size:         72     118     983

  Fragments arranged by size: 

                 983     118      72
 
EaeI y'GGCC_r

Cuts at:      0      84     672     926    1173 
   Size:         84     588     254     247

  Fragments arranged by size: 

                 588     254     247      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1173 
   Size:       1037     136
 
EcoO109I rG'GnC_Cy

Cuts at:      0     322    1173 
   Size:        322     851
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     241     321     441    1173 
   Size:        241      80     120     732

  Fragments arranged by size: 

                 732     241     120      80
 
FspI TGC'GCA

Cuts at:      0     741    1173 
   Size:        741     432
 
FspAI rTGC'GCAy

Cuts at:      0     741    1173 
   Size:        741     432
 
MscI TGG'CCA

Cuts at:      0     674     928    1173 
   Size:        674     254     245

  Fragments arranged by size: 

                 674     254     245
 
NspI r_CATG'y

Cuts at:      0     513     614     740    1173 
   Size:        513     101     126     433

  Fragments arranged by size: 

                 513     433     126     101
 
PciI A'CATG_T

Cuts at:      0     610    1173 
   Size:        610     563
 
PfoI T'CCnGG_A

Cuts at:      0     846    1173 
   Size:        846     327
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315    1096    1128    1173 
   Size:        283      32     781      32      45

  Fragments arranged by size: 

                 781     283      45      32      32
 
SmlI C'TyrA_G

Cuts at:      0     407    1073    1173 
   Size:        407     666     100

  Fragments arranged by size: 

                 666     407     100
 
SspI AAT'ATT

Cuts at:      0     820    1173 
   Size:        820     353
 
StuI AGG'CCT

Cuts at:      0     783    1173 
   Size:        783     390
 
StyI C'CwwG_G

Cuts at:      0     680    1173 
   Size:        680     493
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     344     360    1173 
   Size:        344      16     813

  Fragments arranged by size: 

                 813     344      16
 
TatI w'GTAC_w

Cuts at:      0     293    1173 
   Size:        293     880
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AvaI    BamHI     BanI    BanII     BbsI
     BclI     BglI    BglII     BmrI    BpuEI     BsaI    BsaBI    BseRI
    BseYI Bsp1286I    BspHI    BspMI    BssSI    BstYI   Bsu36I     BtsI
     EaeI  Eco57MI EcoO109I     FalI     FspI    FspAI     MscI     NspI
     PciI     PfoI     PpiI     SmlI     SspI     StuI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI