(Linear) (Six-base) MAPSORT of: KIBB5452.seq  Check: 9563  from: 1  to: 1266



KIBB5452.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1266 
   Size:        820     446
 
AflII C'TTAA_G

Cuts at:      0     576    1266 
   Size:        576     690
 
AflIII A'CryG_T

Cuts at:      0     761    1266 
   Size:        761     505
 
AlwNI CAG_nnn'CTG

Cuts at:      0     481    1266 
   Size:        481     785
 
ApoI r'AATT_y

Cuts at:      0     393    1266 
   Size:        393     873
 
AseI AT'TA_AT

Cuts at:      0     419    1266 
   Size:        419     847
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      24     921    1266 
   Size:         24     897     345

  Fragments arranged by size: 

                 897     345      24
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1009    1266 
   Size:       1009     257
 
BglII A'GATC_T

Cuts at:      0     807     963    1266 
   Size:        807     156     303

  Fragments arranged by size: 

                 807     303     156
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     717    1266 
   Size:        717     549
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     980    1266 
   Size:        355     625     286

  Fragments arranged by size: 

                 625     355     286
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      65    1266 
   Size:         65    1201
 
BsaAI yAC'GTr

Cuts at:      0     668    1266 
   Size:        668     598
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     214     244    1266 
   Size:        214      30    1022

  Fragments arranged by size: 

                1022     214      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      27    1266 
   Size:         27    1239
 
BsmI GAATG_Cn'

Cuts at:      0      54    1266 
   Size:         54    1212
 
BspHI T'CATG_A

Cuts at:      0     135     636    1266 
   Size:        135     501     630

  Fragments arranged by size: 

                 630     501     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1232    1266 
   Size:       1232      34
 
BsrDI GCAATG_nn'

Cuts at:      0     531    1266 
   Size:        531     735
 
BstEII G'GTnAC_C

Cuts at:      0     838    1266 
   Size:        838     428
 
BstYI r'GATC_y

Cuts at:      0     807     963    1034    1266 
   Size:        807     156      71     232

  Fragments arranged by size: 

                 807     232     156      71
 
Bsu36I CC'TnA_GG

Cuts at:      0     271    1266 
   Size:        271     995
 
BtsI GCAGTG_nn'

Cuts at:      0      21    1266 
   Size:         21    1245
 
DraI TTT'AAA

Cuts at:      0     984    1266 
   Size:        984     282
 
DraIII CAC_nnn'GTG

Cuts at:      0     349    1266 
   Size:        349     917
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     766    1266 
   Size:        766     500
 
EarI CTCTTCn'nnn_

Cuts at:      0     495    1266 
   Size:        495     771
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     991    1266 
   Size:        991     275
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     820     980    1266 
   Size:        355     465     160     286

  Fragments arranged by size: 

                 465     355     286     160
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1044    1076    1266 
   Size:       1044      32     190

  Fragments arranged by size: 

                1044     190      32
 
HincII GTy'rAC

Cuts at:      0     206     771    1266 
   Size:        206     565     495

  Fragments arranged by size: 

                 565     495     206
 
NdeI CA'TA_TG

Cuts at:      0     738    1266 
   Size:        738     528
 
NspI r_CATG'y

Cuts at:      0     765     894    1266 
   Size:        765     129     372

  Fragments arranged by size: 

                 765     372     129
 
PciI A'CATG_T

Cuts at:      0     761    1266 
   Size:        761     505
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1072    1104    1266 
   Size:       1072      32     162

  Fragments arranged by size: 

                1072     162      32
 
PstI C_TGCA'G

Cuts at:      0     477    1266 
   Size:        477     789
 
SapI GCTCTTCn'nnn_

Cuts at:      0     495    1266 
   Size:        495     771
 
ScaI AGT'ACT

Cuts at:      0      89    1266 
   Size:         89    1177
 
SexAI A'CCwGG_T

Cuts at:      0     540    1266 
   Size:        540     726
 
SfcI C'TryA_G

Cuts at:      0     473    1266 
   Size:        473     793
 
SmlI C'TyrA_G

Cuts at:      0     576    1266 
   Size:        576     690
 
SnaBI TAC'GTA

Cuts at:      0     668    1266 
   Size:        668     598
 
SspI AAT'ATT

Cuts at:      0     563     781    1266 
   Size:        563     218     485

  Fragments arranged by size: 

                 563     485     218
 
StuI AGG'CCT

Cuts at:      0     276     332    1266 
   Size:        276      56     934

  Fragments arranged by size: 

                 934     276      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     191     876    1266 
   Size:        191     685     390

  Fragments arranged by size: 

                 685     390     191
 
TatI w'GTAC_w

Cuts at:      0      87    1266 
   Size:         87    1179
 

 Enzymes that do cut:

     AcuI    AflII   AflIII    AlwNI     ApoI     AseI     BbsI    BciVI
    BglII     BmrI     BpmI     BsaI    BsaAI    BsaXI    BseRI     BsmI
    BspHI    BspMI    BsrDI   BstEII    BstYI   Bsu36I     BtsI     DraI
   DraIII     DrdI     EarI     EciI  Eco57MI    Hin4I   HincII     NdeI
     NspI     PciI     PpiI     PstI     SapI     ScaI    SexAI     SfcI
     SmlI    SnaBI     SspI     StuI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI